[1]李莉,谭红平,邹志敏,等.抑制S100B表达改善大鼠重型颅脑损伤后继发性损伤[J].中国临床神经外科杂志,2023,28(08):508-512.[doi:10.13798/j.issn.1009-153X.2023.08.009]
　LI Li,TAN Hong-ping,ZOU Zhi-min,et al.Inhibition of S100B expression improves secondary injury after severe traumatic brain injury in adult rats[J].,2023,28(08):508-512.[doi:10.13798/j.issn.1009-153X.2023.08.009]
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抑制S100B表达改善大鼠重型颅脑损伤后继发性损伤()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
28
期数:
2023年08期
页码:
508-512
栏目:
实验研究
出版日期:
2023-08-31

文章信息/Info

Title:
Inhibition of S100B expression improves secondary injury after severe traumatic brain injury in adult rats
文章编号:
1009-153X（2023）08-0508-05
作者:
李莉; 谭红平; 邹志敏; 李琴; 古正涛
510630广州，南方医科大学第三附属医院创伤救治中心（李莉、邹志敏、李琴、古正涛）；510510广州，广东三九脑科医院癫痫中心（谭红平）
Author(s):
LI Li1;  TAN Hong-ping2;  ZOU Zhi-min1;  LI Qin1;  GU Zheng-tao1
1. Department of Treatment Center For Traumatic Injuries, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, China; 2. Epilepsy Center, Guangdong 999 Brain Hospital, Guangzhou 510510, China
关键词:
重型颅脑损伤; S100B蛋白; 继发性肺损伤; 继发性脑损伤; 氧化应激反应; 大鼠
Keywords:
Severe traumatic brain injury;  S100B;  Secondary lung injury;  Secondary brain injury;  Oxidative stress;  Rats
分类号:
A
DOI:
10.13798/j.issn.1009-153X.2023.08.009
文献标志码:
R 651.1+5
摘要:
目的 探讨抑制S100B对大鼠重型颅脑损伤（sTBI）后继发性损伤的影响及机制。方法 采用液压冲击法建立大鼠sTBI模型，腹腔注射ONO-2506抑制S100B表达，免疫印迹法检测S100B表达表达水平，ELISA检测丙二醛（MDA）、超氧化物歧化酶（SOD）变化，干湿重法检查组织含水量，HE染色分析组织病理变化。结果 大鼠血清、脑组织、肺组织S100B蛋白、MDA水平伤后1 h开始上升，伤后6 h达峰值（P<0.05）；而SDO水平后1 h开始降低，伤后6 h最低（P<0.05）。抑制S100表达，明显减轻脑组织和肺组织结构损伤，明显降低脑组织和肺组织含水量（P<0.05），明显降低MDA水平（P<0.05），明显增加SOD水平（P<0.05）。结论 抑制S100B明显减轻大鼠sTBI脑组织和肺组织损伤，其机制可能与抑制氧化应激反应有关。
Abstract:
Objective To investigate the effect of inhibition of S100B expression on secondary injury after severe traumatic brain injury (sTBI) in adults rats. Methods The rat sTBI model was established by hydraulic shock method, the expression of S100B was inhibited by intraperitoneal injection of ONO-2506, the expression level of S100B was detected by western blot, the changes of MDA and SOD were detected by ELISA, the tissues water content was detected by dry and wet weight method, and the histopathological changes were analyzed by HE staining. Results The levels of S100B expression and MDA in serum, brain tissues and lung tissues of rats began to increase 1 h after injury, and reached a peak at 6 h after injury (P<0.05). The level of SDO began to decrease 1 h after injury and was the lowest at 6 h after injury (P<0.05). Inhibition of S100 expression significantly alleviated structural damage of brain tissues and lung tissues, significantly decreased water content and MDA level of brain tissues and lung tissues (P<0.05), and significantly increased SOD level of brain tissues and lung tissues (P<0.05). Conclusions Inhibition of S100B can significantly reduce the secondary damage of brain tissues and lung tissues in adult rats after sTBI, and its mechanism may be related to inhibition of oxidative stress.

参考文献/References:

[1]ZOU Z, LI L, SCHAFER N, et al. Endothelial glycocalyx in traumatic brain injury associated coagulopathy: potential mechanisms and impact [J]. J Neuroinflammation, 2021, 18(1): 134.
[2]KHELLAF A, KHAN DZ, HELMY A. Recent advances in traumatic brain injury [J]. J Neurol, 2019, 266(11): 2878-2889.
[3]ABDELMALIK PA, DRAGHIC N, LING G. Management of moderate and severe traumatic brain injury [J]. Transfusion, 2019, 59(S2): 1529-1538.
[4]TAN H, GUO Q, HUA G, et al. Inhibition of endoplasmic reticulum stress alleviates secondary injury after traumatic brain injury [J]. Neural Regen Res, 2018, 13(5): 827-836.
[5]LI L, TAN H, LIU C, et al. Polydatin prevents the induction of secondary brain injury after traumatic brain injury by protecting neuronal mitochondria [J]. Neural Regen Res, 2019, 14(9): 1573-1582.
[6]张　俊，胡　锦，王海丽，等. 入院血清乳酸水平在重型颅脑损伤早期死亡风险评估中的价值[J]. 中国临床神经外科杂志，2022，27（9）：736-738.
[7]GU Z, LI L, LI Q, et al. Polydatin alleviates severe traumatic brain injury induced acute lung injury by inhibiting S100B mediated NETs formation [J]. Int Immunopharmacol, 2021, 98: 107699.
[8]KERR NA, DE RIVERO VJ, WEAVER C, et al. Enoxaparin attenuates acute lung injury and infla mmasome activation after traumatic brain injury [J]. J Neurotrauma, 2021, 38(5): 646-654.
[9]GINER J, MESA GL, YUS TS, et al. Traumatic brain injury in the new millennium: a new population and new management [J]. Neurologia (Engl Ed), 2022, 37(5): 383-389.
[10]RUI T, LI Q, SONG S, et al. Ferroptosis-relevant mechanisms and biomarkers for therapeutic interventions in traumatic brain injury [J]. Histol Histopathol, 2020, 35: 1105-1113.
[11]SHAHIM P, POLITIS A, VAN DER MERWE A, et al. Neurofilament light as a biomarker in traumatic brain injury [J]. Neurology, 2020, 95(6): e610-e622.
[12]ZHANG H, CHEN W, LIU RN, et al. Notch inhibitor can attenuate apparent diffusion coefficient and improve neurological function through downregulating NOX2-ROS in severe traumatic brain injury [J]. Drug Des Devel Ther, 2018, 12: 3847-3854.
[13]曾子桓，张　灏，陈伟强，等. 颅脑损伤后继发性脑损伤发病机制的研究进展 [J]. 中国临床神经外科杂志，2019，24(12)：777-779.
[14]LIN X, XU Z, WANG P, et al. Role of PiCCO monitoring for the integrated management of neurogenic pulmonary edema following traumatic brain injury: a case report and literature review [J]. Exp Ther Med, 2016, 12(4): 2341-2347.
[15]LIU Y, LU J, WANG X, et al. Erythropoietin-derived peptide protects against acute lung injury after rat traumatic brain injury [J]. Cell Physiol Biochem, 2017, 41(5): 2037-2044.
[16]EASTMAN CL, D'AABROSIO R, GANESH T. Modulating neuroinfla mmation and oxidative stress to prevent epilepsy and improve outcomes after traumatic brain injury [J]. Neuropharmacology, 2020, 172: 107907.
[17]ISMAIL H, SHAKKOUR Z, TABET M, et al. Traumatic brain injury: oxidative stress and novel anti-oxidants such as mitoquinone and edaravone [J]. Antioxidants (Basel), 2020, 9(10): 943.
[18]WANG H, ZHOU X, WU LY, et al. Aucubin alleviates oxidative stress and infla mmation via Nrf2-mediated signaling activity in experimental traumatic brain injury [J]. J Neuroinflammation, 2020, 17(1): 188.
[19]BAECKER J, WARTCHOW K, SEHM T, et al. Treatment with the neurotrophic protein S100B increases synaptogenesis after traumatic brain injury [J]. J Neurotrauma, 2020, 37(8): 1097-1107.
[20]Jones C, Harmon C, Mccann M, et al. S100B outperforms clinical decision rules for the identification of intracranial injury on head CT scan after mild traumatic brain injury [J]. Brain Inj, 2020, 34(3): 407-414.
[21]尹文国，翁山山，赖仕宇，等. 联合GCS评分、CT评分与血清S100B蛋白可评估急性颅脑创伤病人损伤程度及早期预后[J]. 南方医科大学学报，2021，41(4)：543-548.
[22]Park DW, Park SH, Hwang SK. Serial measurement of S100B and NSE in pediatric traumatic brain injury [J]. Childs Nerv Syst, 2019, 35(2): 343-348.
[23]Schmidt AP, Carmona M. Serum biomarkers of brain injury: S100B protein, cognitive dysfunction, and major noncardiac surgery [J]. Braz J Anesthesiol, 2020, 70(6): 570-572.
[25]Kiiski H, Langsjo J, Tenhunen J, et al. S100B, NSE and MMP-9 fail to predict neurologic outcome while elevated S100B associates with milder initial clinical presentation after aneurysmal subarachnoid hemorrhage [J]. J Neurol Sci, 2018, 390: 129-134.

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备注/Memo

备注/Memo:
（2022-10-29收稿，2023-04-04修回）
基金项目：广东省自然科学基金（2019A1515010295；2020A1515010092；2022A1515012350）；南方医科大学第三附属医院院长基金（YM202207；YM202204；YQ202211）；国家自然科学基金（81901997）
通讯作者：谭红平，E-mail：406621369@qq.com
更新日期/Last Update: 2022-08-31