[1]曹瑜,邢丹.阿托伐他汀对大鼠脑缺血再灌注损伤后神经细胞焦亡的影响[J].中国临床神经外科杂志,2024,29(02):92-96104.[doi:10.13798/j.issn.1009-153X.2024.02.007]
　CAO Yu,XING Dan.Effect of atorvastatin on neuronal pyroptosis in rats after cerebral ischemia-reperfusion injury[J].,2024,29(02):92-96104.[doi:10.13798/j.issn.1009-153X.2024.02.007]
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阿托伐他汀对大鼠脑缺血再灌注损伤后神经细胞焦亡的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
29
期数:
2024年02期
页码:
92-96104
栏目:
实验研究
出版日期:
2024-02-28

文章信息/Info

Title:
Effect of atorvastatin on neuronal pyroptosis in rats after cerebral ischemia-reperfusion injury
文章编号:
1009-153X（2024）02-0092-05
作者:
曹瑜; 邢丹
215699江苏，张家港市中医医院药学部（曹瑜、邢丹）
Author(s):
CAO Yu;  XING Dan
Department of Pharmacy, Zhangjiagang Traditional Chinese Medicine Hospital, Zhangjiagang 215699, China
关键词:
脑缺血再灌注损伤; 细胞焦亡; 阿托伐他汀; miR-21
Keywords:
Cerebral ischemia-reperfusion injury;  Atorvastatin;  Pyroptosis;  miR-21
分类号:
R 743
DOI:
10.13798/j.issn.1009-153X.2024.02.007
文献标志码:
A
摘要:
目的 探讨阿托伐他汀（AVT）对大鼠脑缺血再灌注（CIR）损伤后神经细胞焦亡的影响及机制。方法 选取成年雄性SD大鼠60只，随机分为假手术组（Sham组）、模型组（CIR组）、阿托伐他汀组（AVT组）以及miR-21抑制剂组，每组15只。线栓法制备CIR损伤模型。AVT组大鼠缺血后2、14 h给予AVT灌胃[10 mg/(kg·d)]，Sham组大鼠给予等量生理盐水灌胃，miR-21抑制剂组大鼠先脑室注射病毒液（1010 pfu/只）预处理3 d后建立CIR模型并在缺血后2、14 h给予AVT灌胃。CIR后1周，采用Longa法进行大鼠神经功能障碍评分，干湿重法检测大鼠脑组织含水量，HE染色观察大鼠海马神经元形态学变化，qRT-PCR检测大鼠海马组织miR-21表达，免疫印迹法检测海马组织细胞焦亡相关蛋白（Caspase-1、GSDMD、IL-1β和IL-18）的表达。结果 与CIR组相比，AVT组大鼠神经功能评分明显改善（P<0.01），脑组织含水量明显降低（P<0.01），海马组织miR-21表达明显升高（P<0.01）、焦亡相关蛋白cleaved-caspase-1、GSDMD-N、IL-1β和IL-18表达水平明显降低（P<0.01）。与AVT组相比，miR-21抑制剂组大鼠上述指标显著逆转（P<0.05）。结论 AVT能够改善CIR大鼠神经功能损伤，机制可能与上调miR-21表达、抑制神经细胞焦亡相关。
Abstract:
Objective To investigate the effect of atorvastatin (AVT) on neuronal pyroptosis in rats after cerebral ischemia-reperfusion (CIR) injury and the underlying mechanisms. Methods Sixty adult male Sprague-Dawley rats were randomly divided into sham group, CIR group, atorvastatin group (AVT group) and miR-21 inhibitor group, with 15 rats in each group. The CIR model was established by thread embolism method. The rats in the AVT group were given AVT [10 mg/(kg·d)] by gavage at 2 and 14 h after ischemia, the rats in the sham group were given the same amount of saline by gavage, and the rats in the miR-21 inhibitor group received intracerebral injection of virus solution (1010 pfu/rat) for 3 days before ischemia and then given AVT by gavage at 2 and 14 h after ischemia. One week after CIR, the neurological dysfunction score was evaluated by Longa method, the water content of brain tissues was measured by dry-wet weight method, the morphological changes of hippocampal tissues were observed by HE staining, the expressions of miR-21 and pyroptosis-related proteins (Caspase-1, GSDMD, IL-1β and IL-18) in hippocampal tissues were detected by qRT-PCR and Western blotting, respectively. Results Compared with the CIR group, the neurological function score was significantly improved (P<0.01), the water content of brain tissues was significantly decreased (P<0.01), the expression level of miR-21 was significantly increased (P<0.01), and the expression levels of pyroptosis-related proteins were significantly decreased in the AVT group (P<0.01). Compared with the AVT group, the above effects were significantly reversed in the miR-21 inhibitor group (P<0.05). Conclusions AVT can improve the neurological function of CIR rats, and the mechanism may be related to up-regulating the expression of miR-21 and inhibiting neuronal pyroptosis.

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备注/Memo

备注/Memo:
（2023-04-17收稿，2023-12-26修回） 基金项目：2020年江苏省干部保健科研课题（BJ20043） 通信作者：邢　丹，Email：38546959@qq.com
更新日期/Last Update: 2024-02-28