[1]彭 琨 周崇高 夏仁鹏 赵 凡 马体栋 李碧香.LRIG3基因过表达对人神经母细胞瘤细胞系SK-N-MC细胞生物学行为的影响[J].中国临床神经外科杂志,2020,(07):458-461.[doi:10.13798/j.issn.1009-153X.2020.07.014]
 PENG Kun,ZHOU Chong-gao,XIA Ren-peng,et al.Overexpression of LRIG3 on cell biological behaviors of human neuroblastoma cell line SK-N-MC[J].,2020,(07):458-461.[doi:10.13798/j.issn.1009-153X.2020.07.014]
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LRIG3基因过表达对人神经母细胞瘤细胞系SK-N-MC细胞生物学行为的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2020年07期
页码:
458-461
栏目:
实验研究
出版日期:
2020-07-25

文章信息/Info

Title:
Overexpression of LRIG3 on cell biological behaviors of human neuroblastoma cell line SK-N-MC
文章编号:
1009-153X(2020)07-0458-04
作者:
彭 琨 周崇高 夏仁鹏 赵 凡 马体栋 李碧香
410000 长沙,湖南省儿童医院胎儿与新生儿外科(彭 琨、周崇高、夏仁鹏、赵 凡、马体栋、李碧香)
Author(s):
PENG Kun ZHOU Chong-gao XIA Ren-peng ZHAO fan MA Ti-dong LI Bi-xiang.
Department of Neonatal Surgery, Hunan Children's Hospital, Changsha 410000, China
关键词:
神经母细胞瘤SK-N-MC细胞LRIG3基因过表达细胞增殖细胞侵袭细胞凋亡
Keywords:
Neuroblastoma Leucine-rich repeat immunoglobulin-like protein 3 Overexpression Cell biological behavior
分类号:
R 739.43; Q 786
DOI:
10.13798/j.issn.1009-153X.2020.07.014
文献标志码:
A
摘要:
目的 探讨富含亮氨酸重复序列免疫球蛋白样蛋白3(LRIG3)基因过表达对人神经母细胞瘤细胞系SK-N-MC细胞生物学行为的影响。方法 体外培养人神经母细胞瘤细胞系SK-N-MC细胞,分成空白对照组(A组,不转染任何载体或质粒)、载体组(B组,转染载体)和LRIG3过表达组(C组,转染含LRIG3基因过表达质粒)。 荧光定量PCR和免疫印迹法检测LRIG3、Caspase-3和Caspase-9的mRNA和蛋白表达水平。Transwell试剂盒检测细胞侵袭能力,AV-PI试剂盒检测细胞凋亡水平,CCK-8试剂盒检测细胞增殖能力。结果 C组Caspase-3和Caspase-9的mRNA和蛋白表达水平以及细胞凋亡率明显高于A组和B组(P<0.05),细胞增殖率和细胞侵袭能力明显低于A组和B组(P<0.05),而A组和B组之间均无统计学差异(P>0.05)。结论 LRIG3过表达可以抑制人神经母细胞瘤细胞系SK-N-MC细胞增殖和侵袭,促进其凋亡,其机制可能与促进Caspase-3和Caspase-9表达有关
Abstract:
Objective To explore the effect of overexpression of LRIG3 on the cell biological behaviors of human neuroblastoma cell line SK-N-MC. Methods Human neuroblastoma cell line SK-N-MC was cultured in vitro and then were randomly divided into three groups, i.e., control group (group A, did not transfect any vector virus), blank vector group (group B, transfected with blank vector virus) and LRIG3 overexpression group (group C, transfected with LRIG3 overexpression vector lentivirus). The expression levels of LRIG3, Caspase-3 and Caspase-9 were detected by RT-PCR and Western blot. Transwell kit was used to detect cell invasiveness, AV-PI kit was used to detect cell apoptosis and CCK-8 kit was used to detect cell proliferation. Results The expression levels of Caspase-3 and Caspase-9 in group C was significantly higher than those in groups A and B (P<0.05). The cell proliferation rate of group C was significantly lower than those of groups A and group B (P<0.05), and the cell invasion ability of group C was significantly lower than those of groups A and group B (P<0.05). The apoptotic rate in group C was significantly higher than those in groups A and B (P<0.05). Conclusion LRIG3 overexpression can inhibit the proliferation and invasion of human neuroblastoma cell line SK-N-MC, and promote the apoptosis of human neuroblastoma cell line SK-N-MC

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备注/Memo

备注/Memo:
(2019-12-27收稿,2020-04-13修回)通讯作者:李碧香,E-mail:pou5le@163.com
更新日期/Last Update: 2020-07-20