[1]张先斌　张　珑　余振兴　龚存林　龚笑笑　程祖珏　吕　卿.法舒地尔对脑出血大鼠神经功能的影响[J].中国临床神经外科杂志,2020,(08):542-545.[doi:10.13798/j.issn.1009-153X.2020.08.014]
　ZHANG Xian-bin,ZHANG Long,YU Zhen-xing,et al.Effects of fasudil on nerve function in rats after cerebral hemorrhage[J].,2020,(08):542-545.[doi:10.13798/j.issn.1009-153X.2020.08.014]
点击复制

法舒地尔对脑出血大鼠神经功能的影响()
分享到：

《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2020年08期
页码:
542-545
栏目:
实验研究
出版日期:
2020-08-25

文章信息/Info

Title:
Effects of fasudil on nerve function in rats after cerebral hemorrhage
文章编号:
1009-153X（2020）08-0542-04
作者:
张先斌　张　珑　余振兴　龚存林　龚笑笑　程祖珏　吕　卿
330002 南昌，中国人民解放军联勤保障部队第908医院神经外科（张先斌、张　珑、余振兴、龚存林、龚笑笑）；330006 南昌，南昌大学第二附属医院神经外科
Author(s):
ZHANG Xian-bin1;  ZHANG Long1;  YU Zhen-xing1;  GONG Cun-lin1;  GNG Xiao-xiao1;  CHENG Zu-jue2;  LV Qing3.
1. Department of Neurosurgery, The 908th Hospital of Joint Service Support Force, PLA, Nanchang 330002, China; 2. Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China; 3. Department of Neurosurgery, De
关键词:
脑出血; 法舒地尔; 神经功能; 细胞凋亡; 大鼠
Keywords:
Intracerebral hemorrhage;  Fasudil;  Nerve function;  Cell apoptosis;  Rat
分类号:
A
DOI:
10.13798/j.issn.1009-153X.2020.08.014
文献标志码:
R 743.34
摘要:
目的 探讨法舒地尔对脑出血大鼠神经功能的保护作用及机制。方法 将60只SD大鼠随机分为：假手术组、模型组、低剂量法舒地尔组（低剂量组）和高剂量法舒地尔组（高剂量组）；每组15只。通过注射自体脑内动脉血制作脑出血模型，假手术组只暴露硬脑膜，不注射动脉血。低剂量组腹腔注射1 mg/（kg·d）法舒地尔，高剂剂量组腹腔注射10 mg/（kg·d）法舒地尔。模型组和假手术组腹腔注射等体积生理盐水。药物干预4周后，使用Y-迷宫法评价大鼠学习记忆功能，记录大鼠错误反应次数；使用Longa法评价大鼠神经功能。干湿重法测定脑组织含水量，使用硝酸溶解法测定Na+、K+含量，TUNEL染色分析神经细胞凋亡率，免疫印迹法分析凋亡相关蛋白表达（Bax、Bcl-2和Caspase-3）。结果 相比假手术组，模型组大鼠逃离错误次数、Longa神经功能评分、脑组织含水量、脑组织Na+离子水平、神经细胞凋亡率、Caspase-3和Bax蛋白表达水平显著升高（P<0.01），脑组织K+离子水平、Bcl-2蛋白表达水平显著降低（P<0.01）。相比模型组，法舒地尔模型逆转大鼠脑出血后上述变化（P<0.01），而且高剂量法舒地尔明显优于低剂量法舒地尔（P<0.01）。结论 大鼠脑出血后，法舒地尔可以调节神经细胞Na+、K+离子平衡并减少脑组织水肿、抑制神经细胞凋亡，保护大鼠神经功能
Abstract:
Objective To explore the protective effect and the mechanism of fasudil on nerve function of rats after intracerebral hemorrhage (ICH). Methods Sixty rats were randomly divided into four groups, i.e., sham operation group, model group, low-dose fasudil group and high-dose fasudil group, with 15 rats in each group. The ICH model was made by injecting autologous intracerebral arterial blood. In the sham operation group, only the dura mater was exposed and no arterial blood was injected. The rats in low-dose and high-dose groups were intraperitoneally injected with 1 mg/(kg·d) and 10 mg/(kg·d) fasudil, respectively. The rats in model and sham operation groups were intraperitoneally injected with the same volume of normal saline. After 4 weeks of treatment: the Y-maze method was used to evaluate the learning and memory function of rats, and the number of wrong reactions in rats was recorded; the Longa method was used to evaluate the neurological function of rats; the water content of brain tissue was determined by dry and wet weight method; the Na+ and K+ contents were determined by nitric acid dissolution method; the apoptosis rate of nerve cells was analyzed by TUNEL staining, and the apoptosis-related protein expression (Bax, Bcl-2 and Caspase-3) was analyzed by western blotting. Results Compared with the sham operation group, the number of times of error escape, Longa nerve function score, brain tissue water content, brain tissue Na+ level, nerve cell apoptosis rate, and protein expression levels of Caspase-3 and Bax were significantly increased in the model group (P<0.01), but brain tissue K+ level and protein expression level of Bcl-2 were significantly reduced (P<0.01). The fasudil reversed the above changes after ICH in rats (P<0.01), and the high-dose fasudil was significantly better than the low-dose fasudil (P<0.01). Conclusion After ICH in rats, fasudil can adjust the balance of Na+ and K+ in nerve cells, reduce brain edema and nerve cell apoptosis, and protect rat nerve function

参考文献/References:

[1] Gioia L, Klahr A, Kate M, et al. The intracerebral hemorr- hage acutely decreasing arterial pressure trial Ⅱ (ICH ADAPT Ⅱ) protocol [J]. BMC Neurol, 2017, 17(1): 100- 101.
[2] Cai JC, Liu W, Lu F, et al. Resveratrol attenuates neurolo- gical deficit and neuroinflammation following intracerebral hemorrhage [J]. Exp Ther Med, 2018, 15(5): 4131-4138.
[3] 蔡楚伟，张培峰，黄楚明，等. 原发性与继发性基底节区脑 出血的人口学特点、临床及影像学特征研究[J]. 中国实 用医药，2017，12（35）：39-41.
[4] 李丽敏，张昆仑，赵迎春，等. 盐酸法舒地尔对脑出血大鼠 脑血管痉挛的作用研究[J]. 脑与神经疾病杂志，2017，25 （11）：677-681.
[5] 周　昉，党红星，陈应富，等. Rho激酶抑制剂对先天性膈 疝大鼠模型胎仔肺血管重构的作用[J]. 重庆医科大学学 报，2018，43（3）：343-347.
[6] 周喜燕，于　淼，崔换新. 脑出血大鼠模型中血脑屏障通 透性变化与细胞凋亡、炎症及应激反应激活的相关性研 究[J]. 海南医学院学报，2018，24（14）：1299-1302.
[7] 李中华，张　贺，刘　冰，等. 不同压力高压氧对实验性脑 出血大鼠模型血肿周围水肿及MMP-9和TIMP-1表达 的影响[J]. 中国老年学杂志，2014，4（19）：5501-5503.
[8] 吴　巧，马康华，张晓刚，等. Rho激酶抑制剂对大鼠血管 内皮的保护作用及eNOS表达的影响[J]. 解放军医学杂 志，2012，37（11）：1044-1049.
[9] Fujimura M, Vsuki F, Makamura A, et al. Fasudil, a ROCK inhibitor, recovers methylmercury-induced axonal degene- ration by changing microglial phenotype in rats [J]. Toxicol Sci, 2018, 168(1):126-136..
[10] 谢　芹，尤春景. 高压氧对实验性脑出血大鼠的治疗作用 [J]. 中国康复，2004，19（2）：75-77.
[11] 马玉东，宋　君，张海波. 神经干细胞移植对脑外伤大鼠 神经功能及脑组织Bcl-2与Bax表达的影响[J]. 解剖科 学进展，2018，24（1）：19-21，25.
[12] 丁晨雨，胡利双，李　云，等. 低氧胁迫对鲢心肌细胞凋亡 及其调控基因Bax、Bcl-2表达的影响[J]. 淡水渔业， 2018，48（2）：10-15.
[13] 王吉锡，邓伟生，王凤儒，等. 甘草提取物的有效部位诱导 人宫颈癌HeLa细胞株凋亡及对凋亡相关蛋白Caspase- 3和Caspase-9表达的影响[J]. 实用肿瘤学杂志，2017，31 （5）：390-395.
[14] Zhang LC, Li QH, Liu Z, et al. Protective effects of a rho kinase inhibitor on paraquat-induced acute lung injuries in rats [J]. Inflammation, 2018, 41(6): 2171-2183.

相似文献/References:

[1]郑　涛　赵兰夫　高国栋.多囊肾尿毒症期并发脑出血综合治疗1例[J].中国临床神经外科杂志,2016,(05):320.[doi:10.13798/j.issn.1009-153X.2016.05.024]
[2]孔令舜　刘玉光.钻孔引流术治疗阿司匹林相关幕上脑出血的效果与安全性[J].中国临床神经外科杂志,2016,(03):181.[doi:10.13798/j.issn.1009-153X.2016.03.019]
[3]皮水平　刘　杰　黄前樟.长期服用抗凝药物的老年颅脑损伤患者治疗体会[J].中国临床神经外科杂志,2016,(02):117.[doi:10.13798/j.issn.1009-153X.2016.02.022]
[4]张　源　王文浩　林　洪　林俊明　黄　巍　李　君　罗　飞　胡连水.侧脑室后角穿刺术的新方法介绍[J].中国临床神经外科杂志,2016,(01):26.[doi:10.13798/j.issn.1009-153X.2016.01.009]
[5]谢　敏　周小炫 综述　吴成翰 审校.Toll样受体4与脑出血的研究进展[J].中国临床神经外科杂志,2015,(01):55.[doi:10.13798/j.issn.1009-153X.2015.01.019]
[6]王洪流,张爱明,董又坤.同时发生的双侧基底节区出血1例[J].中国临床神经外科杂志,2016,(11):736.[doi:10.13798/j.issn.1009-153X.2016.11.033]
[7]罗　明　段发亮　吴京雷　闵　强　陈晓斌　黄乔春　杨国平.神经导航下内镜手术治疗不典型自发性脑出血14例[J].中国临床神经外科杂志,2017,(01):40.[doi:10.13798/j.issn.1009-153X.2017.01.015]
[8]张如梦　王轶群　陈　甲　薛东章　杨世泉　魏学志　程晋成.脑出血抗栓药物应用研究进展[J].中国临床神经外科杂志,2017,(12):860.[doi:10.13798/j.issn.1009-153X.2017.12.022]
[9]梁智雄　彭　岩　胡　松.预防性应用辛伐他汀对大鼠脑出血后血肿体积和神经功能的影响[J].中国临床神经外科杂志,2018,(05):331.[doi:10.13798/j.issn.1009-153X.2018.05.009]
　LIANG Zhi-xiong,HU Song,PENG Yan..Effects of prophylactic application of simvastatin on hematoma volume and neurological function in rats with intracerebral hemorrhage[J].,2018,(08):331.[doi:10.13798/j.issn.1009-153X.2018.05.009]
[10]陈姣红　刘东媛　张红波　李明昌　熊晓星　陈谦学.成人出血型烟雾病围手术期护理观察[J].中国临床神经外科杂志,2018,(06):437.[doi:10.13798/j.issn.1009-153X.2018.06.020]

备注/Memo

备注/Memo:
（2020-04-02收稿，2020-06-16修回）基金项目：江西省卫生计生委科技新技术推广计划（20183534） （程祖珏）；330400 江西，德安县人民医院神经外科（吕　卿） 通讯作者：张　珑，E-mail：w8vbe1@163.com
更新日期/Last Update: 2020-08-23