[1]范光亮　周云飞　张志龙.胶质瘤组织miR-3653的表达及miR-3653对胶质瘤TG905细胞侵袭、迁移的影响[J].中国临床神经外科杂志,2021,26(03):180-184.[doi:10.13798/j.issn.1009-153X.2021.03.012]
　FAN Guang-liang,ZHOU Yun-fei,ZHANG Zhi-long.Expression of miR-3653 in human glioma tissues and effect of miR-3653 on invasion and migration of glioma TG905 cells[J].,2021,26(03):180-184.[doi:10.13798/j.issn.1009-153X.2021.03.012]
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胶质瘤组织miR-3653的表达及miR-3653对胶质瘤TG905细胞侵袭、迁移的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
26
期数:
2021年03期
页码:
180-184
栏目:
实验研究
出版日期:
2021-03-25

文章信息/Info

Title:
Expression of miR-3653 in human glioma tissues and effect of miR-3653 on invasion and migration of glioma TG905 cells
文章编号:
1009-153X（2021）03-0180-05
作者:
范光亮　周云飞　张志龙
265700 山东龙口，龙口南山养生谷肿瘤医院神经外科（范光亮）；264033 山东烟台，滨州医学院（周云飞、张志龙）
Author(s):
FAN Guang-liang;  ZHOU Yun-fei;  ZHANG Zhi-long
1. Department of Neurosurgery, Longkou Nanshan Health Valley Cancer Hospital, Yantai 265700, China; 2. Binzhou Medical University, Yantai 264033, China
关键词:
胶质瘤; TG8059相比; 微小核糖核酸（microRNA; miRNA）; miR-3653; 细胞侵袭; 细胞迁移; 上皮-间质转化; ZEB2
Keywords:
Glioma;  miR-3653;  Cell invasion;  Cell migration;  Epithelial-mesenchymal transition;  ZEB2
分类号:
R 739.41; Q 786
DOI:
10.13798/j.issn.1009-153X.2021.03.012
文献标志码:
A
摘要:
目的 探讨胶质瘤组织miR-3653表达变化及miR-3653对人胶质瘤TG905细胞侵袭和迁移的影响及其分子机制。方法 收集2017年1月至2019年3月手术切除胶质瘤组织和瘤旁脑组织各25例。体外培养TG905细胞，过表达组转染MiR-3653 mimic质粒，过表达对照组转染NC-MiR-3653 mimic质粒，抑制对照组转染NC-MiR-3653 inhibitor质粒，抑制组转染MiR-3653 inhibitor质粒，miR-3653+ZEB2过表达组同时转染miR-3653 mimic和ZEB2 mimic质粒。利用qRT-PCR及免疫印迹法检测mRNA和蛋白表达；应用Transwell实验和划痕实验检测细胞侵袭和迁移能力。结果 与瘤旁脑组织相比，胶质瘤组织miR-3653表达水平明显降低（P<0.05）。低表达miR-3653显著促进胶质瘤TG905细胞的侵袭及迁移（P<0.05）。过表达miR-3653显著抑制胶质瘤TG905细胞的侵袭及迁移（P<0.05），明显抑制TG905细胞上皮-间质转化分子和ZEB2的表达（P<0.05）；过表达ZEB2，可有效抑制miR-3653过表达的作用（P<0.05）。结论 胶质瘤组织miR-3653呈低表达，通过靶向上调 ZEB2，促进细胞上皮-间质转化及细胞侵袭、迁移能力。
Abstract:
Objective To investigate the expression of miR-3653 in human glioma tissues and the effect of miR-3653 on the cell invasion and migration of human glioma TG905 cells. Methods The expression levels of miR-3653 were detected in glioma tissues and para-glioma tissues obtained from 25 patients with glioma who underwent microsurgery from January 2017 to March 2019. TG905 cells were cultured in vitro. The plasmids of MiR-3653 mimic, SEB2 mimic, and MiR-3653 inhibitor were transfected into the TG905 cells to up-regulate the expression of miR-3653 and ZEB2 and to down-regulate the expression of miR-3653, respectively. qRT-PCR and western blotting were used to detect the mRNA and protien expression. Transwell assay and wound-healing assay were used to detect the invasion and migration of TG905 cells. Results The expression lvel of miR-3653 in glioma tissues was significantly lower than that in para-glioma tissues (P<0.05). Down-regulation of miR-3653 significantly promoted the invasion and migration of TG905 cells (P<0.05).Overexpression of miR-3653 significantly inhibited the invasion and migration of TG905 cells, and signifcantly decreased the mRNA and protein expression levels of the ZEB2 and proteins related to epithelial-mesenchymal transition (EMT) (P<0.05). Overexpression of ZEB2 significantly inhibited the effect of overexprsion of miR-3653 on the TG905 cells (P<0.05). Conclusions The expression of miR-3653 in glioma tissues is low, which may be by targeting up-regulation of ZEB2 to promote EMT and cell invasion and migration.

参考文献/References:

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备注/Memo

备注/Memo:
通讯作者：张志龙，E-mail：347854250@qq.com
更新日期/Last Update: 2021-03-25