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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:

26

期数:

2021年04期

页码:

274-277

栏目:

实验研究

出版日期:

2021-04-25

文章信息/Info

Title:

Differential protective effect of etomidate on retinal ganglion cell survival in adult rats after optic nerve injury

文章编号:

1009-153X（2021）04-0274-04

作者:

徐召溪　胡军民　徐国政　马廉亭

430070 武汉，中国人民解放军中部战区总医院神经外科（徐召溪、胡军民、徐国政、马廉亭）

Author(s):

XU Zhao-xi;  Hu Jun-min;  XU Guo-zheng;  MA Lian-ting.

Department of Neurosurgery, General Hospital of Central Theater Command, PLA, Wuhan 430070, China

关键词:

视网膜神经节细胞; 视神经损伤; 依托咪酯; 成年大鼠

Keywords:

Retinal ganglion cells ;  Optic nerve injury;  Adult rats;  Etomidate

分类号:

R 651.1+5

DOI:

10.13798/j.issn.1009-153X.2021.04.015

文献标志码:

A

摘要:

目的 探讨依托咪酯对成年大鼠视神经损伤后视网膜不同部位视网膜神经节细胞（RGCs）存活的影响。方法 取55只成年雌性SD大鼠，于双侧上丘和顶盖前区及外侧膝状荧光金逆行标记RGCs，然后随机分为6组：正常组（n=5），模型组（n=10），脂肪乳组（n=10；视神经损伤后腹腔注射等体积脂肪乳，1次/d），以及低、中、高剂量依托咪酯组（n=30；视神经损伤后腹腔注射依托咪酯，剂量分别为2、4、6 mg/kg，1次/d）。RGCs标记后5 d，距视神经根部1 mm处切断左侧视神经，7、14 d后采用视网膜平铺技术计数距视盘边缘1、2、3 mm处荧光金逆行标记的RGCs。结果 正常组距视盘边缘1、2、3 mm处RGCs密度分别为（2652±116）个/mm2、（2196±145）个/mm2、（1758±137）个/mm2，越靠近视盘，RGCs密度越高（P<0.05）；但是，模型组视神经损伤后7、14 d，距视盘边缘3 mm处RGCs存活率明显高于1、2 mm处RGCs存活率（P<0.05）。视神经损伤后7 d，低剂量依托咪酯显著增加距视盘边缘1 mm处RGCs存活率（P<0.05），中、高剂量依托咪酯显著增加距视盘边缘1、2、3 mm处RGCs存活率（P<0.05）。视神经损伤后14 d，低剂量和中剂量依托咪酯对距视盘边缘1、2、3 mm处RGCs存活率均无明显影响（P>0.05）；高剂量依托咪酯明显增加距视盘边缘1 mm处RGCs存活率（P<0.05）。结论 大鼠RGCs在视网膜中分布是不均匀的，不同部位RGCs对视神经损伤反应不一样，对依托咪酯治疗敏感性也不一样；距视盘越近，对损伤反应越重，对依托咪酯治疗反应越敏感；依托咪酯剂量越高，对RGCs保护作用范围越广，作用持续时间越长。

Abstract:

Objective To explore the effect of etomidate on the survival of retinal ganglion cells (RGCs) in different parts of retina in adult rats after the optic nerve injury (ONI). Methods Fifty-five adult SD rats were randomly divided into 6 groups: normal group (n=5), model group (n=10) and vehicle group (n=10; intraperitoneal injection of equal volume lipid emulsion, once a day), and low-, medium- and high-dose etomidate groups (n=30; intraperitoneal injection of etomidate at 2, 4, 6 mg/kg, respectively; once a day). Five dats after the RGCs labeled by flurogold in bilateral superior colliculus, pretectal region and lateral geniculate bodies, the left optic nerve was transected 1.0 mm from the optic disc, and the RGCs 1, 2 and 3 mm from the edge of optic disc (1-, 2- and 3-EOD, respectively) were calculated 7 and 14 days wfter the ONI. Results The 1-, 2- and 3-EOD RGC densities of normal rats were (2652±116) cells/mm2, (2196±145) cells/mm2 and (1758±137) cells/mm2, respectively. Closer to the optic disc, the higher the RGC densities in normal rats (P<0.05). However, the survival rate of 3-EOD RGCs was significantly higher than those of 1- and 2-EOD RGCs 7 and 14 days after the ONI (P<0.05) in model group. Seven days after the ONI, low-dose etomidate significantly increased the survival rate of 1-EOD RGCs (P<0.05), and medium- and high-dose etomidate significantly increased the survival rate of 1-, 2- and 3-EOD RGCs (P<0.05). Fourteen days after the ONI, low- and medium-dose etomidate had no significant effect on the survival rate of 1-, 2- and 3-EOD RGCs (P>0.05); high-dose etomidate significantly increased the survival rate of 1-EOD RGCs (P<0.05). Conclusions The distribution of rat RGCs in the retina is uneven. Different RGCs have different responses to the ONI, and their sensitivities to etomidate treatment are also different. The closer to the optic disc, the more serious the injury, and the more sensitive to etomidate treatment. The higher the dose of etomidate, the wider the protective range of RGCs and the longer the duration of the effect.

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更新日期/Last Update: 2021-04-25