[1]李 剑 毛星刚 贺亚龙 董必峰.胶质母细胞瘤FMOD基因非CpG岛DNA甲基化水平与病人预后的关系[J].中国临床神经外科杂志,2021,26(05):336-339.[doi:10.13798/j.issn.1009-153X.2021.05.006]
 LI Jian,MAO Xing-gang,HE Ya-long,et al.Change in DNA methylation of non-CpG islands of FMOD gene in glioblastoma[J].,2021,26(05):336-339.[doi:10.13798/j.issn.1009-153X.2021.05.006]
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胶质母细胞瘤FMOD基因非CpG岛DNA甲基化水平与病人预后的关系()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
26
期数:
2021年05期
页码:
336-339
栏目:
论著
出版日期:
2021-05-25

文章信息/Info

Title:
Change in DNA methylation of non-CpG islands of FMOD gene in glioblastoma
文章编号:
1009-153X(2021)05-0336-04
作者:
李 剑 毛星刚 贺亚龙 董必峰
710032 西安,中国人民解放军空军军医大学第一附属医院神经外科(李 剑、毛星刚、贺亚龙、董必峰)
Author(s):
LI Jian MAO Xing-gang HE Ya-long DONG Bi-feng.
Department of Neurosurgery, Xijing Hospital, The First Affiliated Hospital of Air Force Military Medical University, Xi’an 710032, China
关键词:
胶质母细胞瘤纤调蛋白(FMOD)基因表达非CpG岛DNA甲基化预后
Keywords:
Glioblastoma FMOD Non-CpG island Methylation Prognosis
分类号:
R 739.41; Q 786
DOI:
10.13798/j.issn.1009-153X.2021.05.006
文献标志码:
A
摘要:
目的 探讨纤调蛋白(FMOD)基因非CpG岛区域DNA甲基化在人脑胶质母细胞瘤(GBM)中的改变模式以及与基因表达、临床预后的关系。方法 检索国癌症基因组图谱计划数据库(TCGA)和国国立卫生院下属基因表达数据库(GEO)下载人脑GBM组织基因表达芯片GSE36278、GSE22891及GSE50923。分析FMOD基因的CpG探针[cg26987645和cg03764585,均来自人类全基因组DNA甲基化芯片(Infinium HumanMethylation 27 k和450 k BeadChips),均位于非CpG岛区域(www.illumina.com)]β值(与甲基化水平呈正相关)。结果 与非肿瘤组织相比,GBM组织FMOD基因非CpG岛区域探针cg26987645和cg03764585的β值均显著下降,提示GBM组织FMOD基因发生特异性低甲基化。将CpG岛区域探针cg26987645和cg03764585的β值与mRNA数据进行关联分析发现,DNA甲基化水平与FMOD基因表达水平呈显著负相关(P<0.001)。Cox回归分析显示,随FMOD基因CpG岛区域探针cg26987645和cg03764585的β值增加,GBM病人生存期显著延长(P<0.05)。FMOD基因非CpG岛cg03764585和cg26987645探针分析显示,非G-CIMP亚型病人甲基化水平较G-CIMP亚型病人明显降低(P<0.05),而FMOD基因表达水平明显增高(P<0.05)。结论 本文结果提示,GBM组织FMOD基因非CpG岛区域呈低甲基化表现,与FMOD基因表达和病人生存预后均呈明显负相关。
Abstract:
Objective To investigate the change pattern of DNA methylation of non-CpG island of FMOD gene in glioblastoma (GBM) and its relationship with FMOD gene expression and patients’ survival prognosis. Methods The National Cancer Genome Atlas Database (TCGA) and the National Institute of Health Gene Expression Database (GEO) were searched to download human brain GBM tissue gene expression chips GSE36278, GSE22891 and GSE50923. The β value, which was positively correlated with the methylation level, was analyzed using the FMOD gene CpG probes cg26987645 and cg03764585 which were obtained from human genome-wide DNA methylation chips (Infinium HumanMethylation 27 k and 450 k BeadChips), and were located in the non-island region (www.illumina.com)]. Results Compared with non-tumor tissues, the β values of probes cg26987645 and cg03764585 in the non-CpG island region of the FMOD gene of GBM tissues were significantly decreased, suggesting that the FMOD gene of GBM tissues was specifically hypomethylated. Correlation analysis between the β values of probes cg26987645 and cg03764585 in the CpG island region and the mRNA data found that the DNA methylation level was significantly negatively correlated with the FMOD gene expression level (P<0.001). Cox regression analysis showed that as the β values of the probes cg26987645 and cg03764585 in the CpG island region of the FMOD gene increased, the survival time of GBM patients was significantly prolonged (P<0.05). The analysis of FMOD gene non-CpG island probes cg03764585 and cg26987645 showed that the methylation level of non-G-CIMP subtype patients was significantly lower than that of G-CIMP subtype patients (P<0.05), while the FMOD gene expression level was significantly increased (P<0.05). Conclusions Our results suggest that the non-CpG island regions of FMOD gene in GBM tissues are hypomethylated, which is significantly negatively correlated with FMOD gene expression and patient survival prognosis.

参考文献/References:

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更新日期/Last Update: 2021-05-25