[1]周有东　马金阳　黄　松　胡火军　符常涛　袁　高　郭金满　汪　雷.舒芬太尼对大鼠蛛网膜下腔出血后脑损伤的保护作用[J].中国临床神经外科杂志,2021,26(07):531-536.[doi:10.13798/j.issn.1009-153X.2021.07.012]
　ZHOU You-dong,MA Jin-yang,HUANG Song,et al.Effect of sufentanil on brain injury after subarachnoid hemorrhage in adult rats[J].,2021,26(07):531-536.[doi:10.13798/j.issn.1009-153X.2021.07.012]
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舒芬太尼对大鼠蛛网膜下腔出血后脑损伤的保护作用()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
26
期数:
2021年07期
页码:
531-536
栏目:
实验研究
出版日期:
2021-07-25

文章信息/Info

Title:
Effect of sufentanil on brain injury after subarachnoid hemorrhage in adult rats
文章编号:
1009-153X（2021）07-0531-06
作者:
周有东　马金阳　黄　松　胡火军　符常涛　袁　高　郭金满　汪　雷
441021 湖北宜昌，三峡大学神经病学研究所/宜昌市中心人民医院神经外科（周有东、马金阳、黄　松、胡火军、符常涛、袁　高、郭金满、汪　雷）
Author(s):
ZHOU You-dong;  MA Jin-yang;  HUANG Song;  HU Huo-jun;  FU Chang-tao;  YUAN Gao;  GUO Jin-man;  WANG Lei.
Institute of Neurology, China Three Gorges University; Department of Neurosurgery, Yichang Central People’s Hospital, Yichang 441021, China
关键词:
蛛网膜下腔出血; 脑损伤; 舒芬太尼; 炎症反应; 氧化应激; 细胞凋亡; 大鼠
Keywords:
Subarachnoid hemorrhage;  Brain injury;  Sufentanil;  Inflammation;  Oxidative stress;  Cell apoptosis;  Rat
分类号:
R 743.9
DOI:
10.13798/j.issn.1009-153X.2021.07.012
文献标志码:
A
摘要:
目的 探讨舒芬太尼对大鼠蛛网膜下腔出血（SAH）后脑损伤的作用及其机制。方法 取60只成年SD大鼠随机分为假手术组、模型组和舒芬太尼组，每组20只。采用血管内穿孔法建立SAH模型，舒芬太尼组建模后6 h尾静脉注射舒芬太尼溶液（5 μg/kg，1次/d，连续3周），假手术组和模型组尾静脉注射等量生理盐水。造模后1、2、21 d，采用改良改良神经严重性量表（mNSS）评分评估神经功能；末次神经功能评估结束后，采用湿干重比法测定脑含水量，TUNEL染色检测神经元凋亡率，EILSA法测定脑组织MDA、SOD、GSH-PX、IL-1β、IL-6和TNF-α的含量，免疫印迹法检测Nrf2、HO-1、NF-κB p65、BCL-2和cleaved-caspase-3蛋白的表达。结果 与假手术组相比，模型组大鼠mNSS显著增高（P<0.001），脑含水量显著增加（P<0.05），神经元细胞凋亡率显著增加（P<0.001），脑组织MDA、TNF-α、IL-1β、IL-6、NF-kB p65和cleaved-caspase-3水平显著升高（P<0.01），而脑组织SOD、GSH-Px、BCL-2、Nrf2和HO-1水平显著降低（P<0.05）。与模型组相比，舒芬太尼组大鼠上述改变明显改善（P<0.05）。结论 舒芬太尼对SAH后脑损伤有保护作用，其机制可能其抗细胞凋亡、抗氧化应激和抗炎症反应等作用有关。
Abstract:
Objective To explore the effect of sufentanil on the brain injury after subarachnoid hemorrhage (SAH) in adult rats. Methods Sixty adult SD rats were randomly divided into sham operation group, model group and sufentanil group, with 20 rats in each group. The SAH model was established by intravascular perforation. The rats of sufentanil group were injected with sufentanil for 21 days (5 μg/kg, once a day) 6 hours after SAH, and the rats of sham operation and model groups were injected with the same amount of normal saline. The modified modified neurological severity scale (mNSS) score was used to assess neurological function 1, 2 and 21 days after SAH. After the final neurofunction evaluation, the wet-to-dry weight ratio method was used to assess the brain water content, TUNEL staining was used to detect the neuronal apoptosis rate, EILSA method was used to determine the levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), interleukin (IL) -1β, IL-6 and tumor necrosis factor (TNF-α), and western blotting was used to detect the protein expresson of nuclear factor E2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1), nuclear transcription factor-κB (NF-κB) p65, B-cell lymphoma 2 (BCL-2) and cleaved-caspase-3. Results Compared with the sham operation group, the mNSS score, the brain water content, the neuronal apoptosis rate, and the levels of MDA, TNF-α, IL-1β, IL-6, NF-kB p65 and cleaved-caspase-3 were significantly increased in the model group (P<0.05), while the levels of SOD, GSH-Px, BCL-2, Nrf2 and HO-1 were significantly reduced (P<0.05). Compared with the model group, the above-mentioned changes in the sufentanil group were significantly improved (P<0.05). Conclusions Shufentanyl has a protective effect on the brain injury after SAH in adult rats, which may be related to the anti-apoptosis, antioxidant stress and anti-inflammatory effects of shufentanyl.

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备注/Memo

备注/Memo:
通讯作者：汪　雷，E-mai：onedy@sina.com
更新日期/Last Update: 2021-07-25