[1]白　磊　陈　晨　李志磊　霍国进　冯　毅　严飞平.人参皂甙Rh2对人胶质瘤细胞U87MG凋亡的影响[J].中国临床神经外科杂志,2019,(03):155-158.[doi:10.13798/j.issn.1009-153X.2019.03.010]
　BAI Lei,CHEN Chen,LI Zhi- lei,et al.Effects of genoside Rh2 on the apoptosis of human brain glioma cells U87MG[J].,2019,(03):155-158.[doi:10.13798/j.issn.1009-153X.2019.03.010]
点击复制

人参皂甙Rh2对人胶质瘤细胞U87MG凋亡的影响()
分享到：

《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2019年03期
页码:
155-158
栏目:
论著
出版日期:
2019-03-20

文章信息/Info

Title:
Effects of genoside Rh2 on the apoptosis of human brain glioma cells U87MG
文章编号:
1009-153X（2019）03-0155-04
作者:
白　磊　陈　晨　李志磊　霍国进　冯　毅　严飞平
作者单位：719000 陕西，榆林市第一医院榆林院区神经外科（白　磊、陈　晨、李志磊、霍国进、冯　毅、严飞平）
Author(s):
BAI Lei;  CHEN Chen;  LI Zhi- lei;  HUO Guo- jin;  FENG Yi;  YAN Fei- ping.
Department Of Neurosurgery, Yulin Municipal First Hospital, Yulin 719000, China
关键词:
人胶质瘤细胞; U87MG; 人参皂甙Rh2; 细胞凋亡; 细胞内钙离子浓度
Keywords:
Genoside Rh2;  Human brain glioma cells;  U87MG cells;  Apoptosis;  Intracellular calcium concentration
分类号:
R 739.41
DOI:
10.13798/j.issn.1009-153X.2019.03.010
文献标志码:
A
摘要:
目的 观察人参皂甙Rh2对胶质瘤细胞凋亡的影响并初步探讨其可能机制。方法 将培养的人胶质瘤细胞U87MG随机分为人参皂甙Rh2组、人参皂甙Rh2+尼莫地平组和对照组。人参皂甙Rh2组在常规培养细胞时加入20 μg/ml的人参皂甙Rh2，人参皂甙Rh2+尼莫地平组在人参皂甙Rh2组培养细胞时加入浓度为10 μmol/L的尼莫地平。利用流式细胞仪检测U87MG细胞凋亡，利用激光共聚焦显微镜和流式细胞仪检测U87MG细胞内钙离子浓度。结果 与对照组相比，人参皂甙Rh2促进U87MG细胞凋亡（P<0.05），且增加细胞内游离钙离子浓度（P<0.05）；尼莫地平显著减少人参皂甙Rh2引起的U87MG细胞凋亡（P<0.05）。结论 人参皂甙Rh2可以通过增加细胞内游离钙离子浓度促进U87MG细胞凋亡。
Abstract:
Objective To investigate the effects of genoside Rh2 on the apoptosis of human brain glioma cells and its mechanism. Methods Human brain glioma cells U87MG were randomly divided into two groups, i.e. experimental group in which U87MG cells were cultured in the medium containing 20 μg/ml genoside Rh2 and control group in which U87MG cells were cultured in the medium containing isodose physiological salt solution. The apoptosis of U87MG cells was determined by flow cytometry. And then the intracellular calcium concentration of U387MG cells was measured by laser scanning confocal microscope and flow cytometry. Results The rate of U87MG cells apoptosis and intracellular calcium concentration were significantly higher in the experimental group than those in the control group (P<0.05). Moreover the intracellular calcium concentration and the apoptosis rate of U87MG cells raised by genoside Rh2 were significantly decreased by nifedipine [(a specitic inhibitor of Ca++ channel (P<0.05)]. Conclusion Genoside Rh2 can promote apoptosis of human brain glioma U87MG cells by the up-regulation of intracellular calcium concentration.

参考文献/References:


[1] Jue TR, Sena ES, Macleod MR, et al. A systematic review and meta-analysis of topoisomerase inhibition in preclinicalglioma models [J]. Oncotarget, 2018, 9(13): 11387-11401.
[2] Tsymbal DO, Minchenko DO, Kryvdiuk Ⅳ, et al. Expressionof proliferation related transcription factor genes in U87glioma cells with IRE1 knockdown: upon glucose and glu-tamine deprivation [J]. Fiziol Zh, 2016, 62(1): 3-15.
[3] 于泽奇，程世翔，涂　悦，等. Ki-67、Bcl-2、Bax和Cas-pase-3在脑胶质瘤组织中的表达及临床意义[J]. 中国医药导报，2017，14（5）：9-12.
[4] 呼铁民，孙　影，卢庆韬. ADAM17、EGFR和Ki-67在脑胶质瘤中的表达及意义[J]. 中国医药导报，2016，13（5）：21-24.
[5] 侯　亮，冯海利. 脑胶质瘤组织中P-gp、MRP、BCRP的表达及临床意义[J]. 中国医药导报，2016，14（35）： 12-15.
[6] Moschovos C, Papatheodoropoulos C. The L-type voltage-dependent calcium channel long-term potentiation is higherin the dorsal compared with the ventral associational/com-missural CA3 hippocampal synapses [J]. Neurosci Res, 2016, 106(11): 62-65.
[7] Sun Z, Cao X, Zhang Z, et al. Simulated microgravity inhi-bits L-type calcium channel currents partially by the up-regulation of miR-103 in MC3T3-E1 osteoblasts [J]. Sci Rep, 2015, 5: 8077.
[8] 姚兴军，洪新雨，刘兴吉，等. 人参皂甙Rh2对脑胶质细胞瘤侵袭性的影响[J]. 中国实验诊断学，2005，21： 36-39.
[9] 朱中生，李锦玉，王相智，等. 人参皂甙Rh2对大鼠主动脉TIMP-1、VEGF表达的影响[J]. 现代中西医结合杂志，2017，26（22）： 2411-2413.
[10] 李雪甫，刘艳文，王永实. 人参皂甙Rh2联合顺铂对食管癌细胞的杀伤作用[J]. 现代肿瘤医学，2014，22（3）： 516-518
[11] Shen F, Guo Q, Hu Q, et al. RelB, a good prognosis predic-tor, links cell-cycle and migration to glioma tumorigenesis [J]. Oncol Lett, 2018, 15(4): 4404-4410.
[12] Thust SC, Heiland S, Falini A, et al. Glioma imaging in Europe: a survey of 220 centres and recommendations for best clinical practice [J]. Eur Radiol, 2018, 3 (4): 216-219.
[13] 江　山，陈　莺，房德芳. 人参皂甙Rh2抗海马脑片H2O2损伤作用及与GABAA受体的关系[J]. 中药药理与临床，2016，6（2）：38-42.
[14] Park-JA, Kim-KW, Kim-SI. Caspase specifically cleaves p21 WAF1/CIP1 in the earlier stage of apotosis in SK-HEP-1 human hepatoma cells [J]. Eur-J-Biochem, 1998, 257(1): 242-248.
[15] Zhu P, Hu S, Jin Q, et al. Ripk3 promotes ER stress-induced necroptosis in cardiac IR injury: A mechanism involvingcalcium overload/XO/ROS/mPTP pathway [J]. Redox Biol, 2018, 16(9): 157-168.
[16] Santulli G, Lewis D, des Georges A, et al. ryanodine recep-tor structure and function in health and disease [J]. Subcell Biochem, 2018, 87(3): 329-352.
[17] Han S, Lu Q, Wang N. Apr3 accelerates the senescence of human retinal pigment epithelial cells [J]. Mol Med Rep, 2016, 13(4): 3121-3126.

备注/Memo

备注/Memo:
通讯作者：严飞平，E-mail：43085651@qq.com
更新日期/Last Update: 2019-03-25