[1]杨　华　何强华　张爱华　李　扬　陈　兵.丹参酮ⅡA通过抑制TLR4/NF-κB途径减轻糖氧剥夺对大鼠脑微血管内皮细胞的炎症损伤[J].中国临床神经外科杂志,2020,(09):610-612.[doi:10.13798/j.issn.1009-153X.2020.09.011]
　YANG Hua,HE Qiang-hua,ZHANG Ai-hua,et al.Tanshinone ⅡA relieves inflammatory damage of cerebral microvascular endothelial cells induced by oxygen glucose deprivation via inhibition of TLR4/NF-κB pathway[J].,2020,(09):610-612.[doi:10.13798/j.issn.1009-153X.2020.09.011]
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丹参酮ⅡA通过抑制TLR4/NF-κB途径减轻糖氧剥夺对大鼠脑微血管内皮细胞的炎症损伤()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2020年09期
页码:
610-612
栏目:
实验研究
出版日期:
2020-09-20

文章信息/Info

Title:
Tanshinone ⅡA relieves inflammatory damage of cerebral microvascular endothelial cells induced by oxygen glucose deprivation via inhibition of TLR4/NF-κB pathway
文章编号:
1009-153X（2020）09-0610-03
作者:
杨　华　何强华　张爱华　李　扬　陈　兵
524005 广东湛江，广东医科大学研究生院（杨　华、陈　兵）；524005 广东湛江，中国人民解放军南部战区海军第一医院神经外科（杨　华、何强华、张爱华、李　扬）
Author(s):
YANG Hua1; 2;  HE Qiang-hua2;  ZHANG Ai-hua2;  LI Yang2;  CHEN Bing1.
1. Graduate School, Guangdong Medical University, Zhanjiang 524005, China; 2. Department of Neurosurgery, The First Naval Hospital of Southern Theater Command, PLA, Zhanjiang 524005, China
关键词:
缺血型脑卒中; 脑微血管内皮细胞; 糖氧剥夺; 炎症因子; 丹参酮ⅡA; 大鼠
Keywords:
Cerebral microvascular endothelial cells;  Oxygen glucose deprivation;  Inflammation;  Tanshinone ⅡA;  Rat
分类号:
A
DOI:
10.13798/j.issn.1009-153X.2020.09.011
文献标志码:
R 743
摘要:
目的 探讨丹参酮ⅡA对大鼠脑微血管内皮细胞糖氧剥夺炎症损伤的影响及其机制。方法 原代培养雄性SD大鼠脑微血管内皮细胞，分为对照组、糖氧剥夺组和丹参酮ⅡA组。对照组用DMEM培养，糖氧剥夺组细胞用无糖DMEM培养，丹参酮ⅡA组用在无糖DMEM基础上加用含8 μg/ml丹参酮ⅡA培养。利用CCK8检测细胞活力，酶联免疫吸附试验检测细胞炎症因子IL-6和TNF-α水平；实时PCR和免疫印迹法检测细胞TLR4和NF-κB mRNA和蛋白表达水平。结果 与对照组相比，糖氧剥夺组细胞活力明显降低（P<0.05），IL-6和TNF-α水平明显增加（P<0.05），TLR4和NF-κB mRNA和蛋白表达均明显增加（P<0.05）；丹参酮ⅡA明显抑制糖氧剥夺作用（P<0.05），但未恢复到对照组水平（P<0.05）。结论 丹参酮ⅡA可减轻糖氧剥夺对大鼠脑微血管内皮细胞的炎症损伤，其作用机制可能与抑制TLR4-NF-κB信号通路有关
Abstract:
Objective To investigate the effect of tanshinone ⅡA on the inflammatory damage of cerebral microvascular endothelial cells (CMECs) induced by oxygen glucose deprivation (OGD). Methods The primary CMECs were cultured which were isolated from SD rats brain tissues and randomly divided into control group in which the CMECs were cultured in the conventional DMEM medium, OGD group in which the CMECs were cultured in the DMEM sugar-free medium, and tanshinone ⅡA group in which the CMECs were treated with 8 μg/ml tanshinone Ⅱ during OGD treatment. Cell activity was detected by CCK8 method. Inflammatory cytokines inlcuding IL-6 and TNF-α were detected by ELISA method. TLR4 and NF-κB mRNA and protein expressions were detected by real-time PCR and Western blot, respectively. Results Compared with control group, the CMECs activity was significantly lower (P<0.05), the levels of IL-6 and TNF-α in culture mediem were significantly higher (P<0.05), and TLR4 and NF-κB mRNA and protein expression levels were significantly higher in OGD group (P<0.05). Tanshinone ⅡA significantly inhibited the effects caused by OGD(P<0.05). Conclusion The results suggest that tanshinone ⅡA could relieve the inflammatory damage of CMECs induced by OGD, and its mechanism may be related to the inhibition of TLR4-NF-κB signaling pathway

参考文献/References:

[1] Del Brutto VJ, Chaturvedi S, Diener HC, et al. Antithrom- botic terapy to prevent recurrent strokes in ischemic cere- brovascular disease: JACC scientific expert panel [J]. J Am Coll Cardiol, 2019, 74(6): 786-803.
[2] Yang H, Xi X, Zhao B, et al. KLF4 protects brain microvas- cular endothelial cells from ischemic stroke induced apop- tosis by transcriptionally activating MALAT1 [J]. Biochem Biophys Res Commun, 2018, 495(3): 2376-2382.
[3] Cai M, Guo Y, Wang S, et al. Tanshinone IIA elicits neuro- protective effect through activating the nuclear factor erythroid 2-related factor-dependent antioxidant response [J]. Rejuvenation Res, 2017, 20(4): 286-297.
[4] 李德川，鲍秀琦，孙　华，等. 丹参酮ⅡA对缺血性脑中风 的神经保护作用研究进展[J]. 药学学报，2015，50（6）： 635-639.
[5] 秦锦标，刘高飞，朱　敏. 脑卒中与外周免疫抑制[J]. 中国 临床研究，2018，31（07）： 981-983.
[6] 黄婷婷，李　妍，张月蔓，等. 双光子活体成像技术检测缺 血性脑卒中超早期血脑屏障损伤[J]. 上海交通大学学报 （医学版），2019，39（9）： 998-1003.
[7] 王世召，张立民，张云鹤，等. 血脑屏障与免疫炎症反应 [J]. 中国临床神经外科杂志，2015，20（7）： 439-442.
[8] Dong X, Gao J, Zhang CY, et al. Neutrophil membrane- derived nanovesicles alleviate inflammation to protect mouse brain injury from ischemic stroke [J]. ACS Nano, 2019, 13(2): 1272-1283.
[9] 段媛媛，郭振丰，李雪连. 丹参酮ⅡA治疗心血管疾病研 究机制新进展[J]. 中国临床药理学杂志，2016，32（19）： 1817-1820.
[10] Wang XX, Yang JX, Pan YY, et al. Protective effects of tanshinone ⅡA on endothelial progenitor cells injured by tumor necrosis factor-α [J]. Mol Med Rep, 2015, 12(3): 4055-4062.
[11] Tang C, Xue HL, Bai CL, et al. Regulation of adhesion molecules expression in TNF-α-stimulated brain micro- vascular endothelial cells by tanshinone IIA: involvement of NF-κB and ROS generation [J]. Phytother Res, 2011, 25 (3): 376-380.
[12] Leitner GR, Wenzel TJ, Marshall N, et al. Targeting toll- like receptor 4 to modulate neuroinflammation in central 、 nervous system disorders [J]. Expert Opin Ther Targets, 2019, 23(10): 865-882.
[13] 谢　敏，周小炫. Toll样受体4与脑出血的研究进展[J]. 中国临床神经外科杂志，2015（1）： 55-57.
[14] Tao X, Sun X, Yin L, et al. Dioscin ameliorates cerebral ischemia/reperfusion injury through the downregulation of TLR4 signaling via HMGB-1 inhibition [J]. Free Radic Biol Med, 2015, 84: 103-115.
[15] Tajalli-Nezhad S, Karimian M, Beyer C, et al. The regula- tory role of Toll-like receptors after ischemic stroke: neurosteroids as TLR modulators with the focus on TLR2/4 [J]. Cell Mol Life Sci, 2019, 76(3): 523-537.

备注/Memo

备注/Memo:
（2019-12-08收稿，2020-03-12修回）通讯作者：陈　兵，E-mail：toxiaodaizi@163.com
更新日期/Last Update: 2020-09-20