[1]武朋磊吴赞孟繁成杨廷舰.颅内动脉瘤破裂关键基因的生信分析[J].中国临床神经外科杂志,2022,27(02):95-99.[doi:10.13798/j.issn.1009-153X.2022.02.009]
　WU Peng-lei,WU Zan,MENG Fan-cheng,et al.Analysis of key genes mediating the rupture of intracranial aneurysms based on WGCNA algorithm[J].,2022,27(02):95-99.[doi:10.13798/j.issn.1009-153X.2022.02.009]
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颅内动脉瘤破裂关键基因的生信分析()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
27
期数:
2022年02期
页码:
95-99
栏目:
论著
出版日期:
2022-02-25

文章信息/Info

Title:
Analysis of key genes mediating the rupture of intracranial aneurysms based on WGCNA algorithm
文章编号:
1009-153X（2022）02-0095-05
作者:
武朋磊吴赞孟繁成杨廷舰
261000 山东潍坊，潍坊医学院附属医院神经外科（武朋磊、吴赞、孟繁成、杨廷舰）
Author(s):
WU Peng-lei;  WU Zan;  MENG Fan-cheng;  YANG Ting-jian.
Department of Neurosurgery, Affiliated Hospital of Weifang Medical College, Weifang 261000, China
关键词:
颅内动脉瘤; WGCNA算法; 生物信息学; 基因表达
Keywords:
Intracranial aneurysm;  Rupture;  WGCNA algorithm;  Bioinformatics analysis;  Gene expression
分类号:
R 743.9; Q 786
DOI:
10.13798/j.issn.1009-153X.2022.02.009
文献标志码:
A
摘要:
目的 探讨颅内动脉瘤破裂的关键基因。方法采用生信分析方法，GEO数据库下载数据集GSE13353、GSE15629、GSE54083，R语言筛选差异表达基因（DEG），WGCNA算法分析动脉瘤破裂关键基因，采用GO和KEGG分析关键基因的生物学功能，GSEA软件进行基因富集分析。应用GSE122897数据集进行验证。结果共筛选出49个DEG，其中28个上调，21个下调。WGCNA算法、GO和KEGG分析结果显示，颅内动脉瘤破裂主要与炎症和免疫反应有关。枢纽基因和关键基因筛选显示，VNN2和CXCR4在颅内动脉瘤破裂过程中起重要作用。结论我们使用WGCNA的3个GEO数据集构建破裂和未破裂动脉瘤的共表达网络，筛选出介导颅内动脉瘤破裂的关键基因，这些关键基因与炎症和免疫反应密切相关。这提示炎症和免疫反应可能在颅内动脉瘤破裂过程中起重要作用。
Abstract:
Objective To explore the key genes of intracranial aneurysm rupture. Methods Bioinformatics analysis method was used to analyze the key genes of intracranial aneurysm rupture. Data sets GSE13353, GSE15629, GSE54083 were downloaded from GEO database. R language was used to screen differentially expressed genes (DEGs). WGCNA algorithm was used to analyze the key genes of aneurysm rupture. GO and KEGG analyses were used to analyze the biological functions of the key genes. GSEA software was used to analyze the gene enrichment. The GSE122897 dataset was applied for validation. Results A total of 49 DEGs were screened out, of which 28 were up-regulated and 21 were down-regulated. The results of WGCNA algorithm, GO and KEGG analyses showed that intracranial aneurysm rupture was mainly related to inflammation and immune response. Screening of hub genes and key genes revealed that VNN2 and CXCR4 play important roles in the rupture of intracranial aneurysms. Conclusions We constructed a co-expression network of ruptured and unruptured intracranial aneurysms using 3 GEO datasets, and screen out key genes that mediate rupture of intracranial aneurysms, which are closely related to inflammation and immune responses. Our resuelts suggest that inflammation and immune responses may play an important role in the rupture of intracranial aneurysms.

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备注/Memo

备注/Memo:
通讯作者：杨廷舰，E-mail：1262823739@163.com
更新日期/Last Update: 1900-01-01