[1]陈　曦　陈　娟　谢蕊繁　徐　钰　胡　航　李　然　叶　飞　雷　霆.人脑垂体生长激素腺瘤PKCδ、ERK1/2、gsp癌基因的表达关联性分析[J].中国临床神经外科杂志,2015,(04):225-227.[doi:10.13798/j.issn.1009-153X.2015.04.011]
　CHEN Xi,CHEN Juan,XIE Rui-fan,et al.Expressions gsp oncogene, PKCδ and ERK1/2 in human somatotrophinomas and relationship among them[J].,2015,(04):225-227.[doi:10.13798/j.issn.1009-153X.2015.04.011]
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人脑垂体生长激素腺瘤PKCδ、ERK1/2、gsp癌基因的表达关联性分析()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2015年04期
页码:
225-227
栏目:
论著
出版日期:
2015-04-30

文章信息/Info

Title:
Expressions gsp oncogene, PKCδ and ERK1/2 in human somatotrophinomas and relationship among them
文章编号:
1009-153X（2015）04-0225-03
作者:
陈　曦　陈　娟　谢蕊繁　徐　钰　胡　航　李　然　叶　飞　雷　霆
430030 武汉，华中科技大学同济医学院附属同济医院神经外科（陈　曦、陈　娟、谢蕊繁、徐　钰、胡　航、李　然、叶　飞、雷　霆）
Author(s):
CHEN Xi;  CHEN Juan;  XIE Rui-fan;  XU Yu;  HU Hang;  LI Ran;  YE Fei;  LEI Ting.
Department of Neurosurgery, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan 430030, China
关键词:
垂体生长激素腺瘤; gsp癌基因; PKCδ; ERK1/2
Keywords:
Somatotrophinoma;  Gsp oncogene;  PKCδ;  ERK1/2;  Expression
分类号:
R739.41;Q786
DOI:
10.13798/j.issn.1009-153X.2015.04.011
文献标志码:
A
摘要:
目的 研究人脑垂体生长激素腺瘤组织中蛋白激酶Cδ亚型（PKCδ）、细胞外调节蛋白激酶1/2（ERK1/2）、Gs蛋白2亚基因点突变（gsp癌基因）的个体表达的关联性。方法 收集54例垂体生长激素腺瘤组织，行免疫组化检测PKCδ、ERK1/2蛋白的表达差异；通过PCR法检测gsp突变情况。结果 54例标本中，gsp癌基因（+）11例，gsp癌基因（-）43例；PKCδ表达阳性率为50%，ERK1/2表达阳性率为51.9%。11例gsp癌基因（+）标本PKCδ表达阳性率（45.5%）与gsp癌基因（-）标本表达阳性率（51.2%）无统计学差异（P>0.05）；gsp癌基因（+）标本ERK1/2表达阳性率（36.4%）与gsp癌基因（-）标本表达阳性率（55.8%）无统计学差异（P>0.05）；PKCδ（+）标本ERK1/2表达阳性率（74.1%）明显高于PKCδ（-）标本表达阳性率（29.6%，P<0.05）。结论 人脑垂体生长激素腺瘤内PKCδ与ERK1/2的表达呈正相关，而PKCδ及ERK1/2的表达与gsp突变无明显相关性。
Abstract:
Objective To investigate the expressions of gsp oncogene, PKCδ and ERK1/2 in human somatotrophinomas (HSTs) and relationship among them. Methods The expressions of PKCδ and ERK1/2 were detected by immunohistochemcial technique in 54 samples of HSTs, in which the expression of DNA of gsp oncogene was determined by polymerase chain reaction (PCR). Results The expression of gsp oncogene was positive in 11 and negative in 43 of 54 samples of HSTs. The positive rates of PKCδ and ERK1/2 expressions were 50% (27/54) and 51.85% (28/54) respectively in 54 samples of HSTs. The positive rates of PKCδ and ERK1/2 expressions were 45.45% (5/11) and 36.36% (4/11) respectively in 11 samples of HSTs with the positive expression of gsp oncogene. The positive rates of PKCδ and ERK1/2 expression were 51.16% (22/43) and 55.81% (24/43) respectively in 43 samples of HSTs with the negative expression of gsp oncogene. The positive rate of ERK1/2 expression was 74.07% (20/27) in 27 samples of HSTs with the positive expression of PKCδ, and the positive rate of ERK1/2 was 29.63% (8/27) in 27 samples of HSTs with the negative expression of PKCδ. The positively rate of ERK1/2 expression was significantly higher in HSTs with positive PKCδ than that in HSTs with negative PKCδ (P<0.05). Conclusion The expression of PKCδ is positively related to the expression of ERK1/2, and the expressions of PKCδ and ERK1/2 are insignificantly related to the expression of gsp oncogene in HSTs.

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备注/Memo

备注/Memo:
基金项目：卫生部国家临床重点专科建设项目；国家自然科学基金（81270865）
通讯作者：雷　霆，E-mail：tlei@tjh.tjmu.edu.cn
更新日期/Last Update: 2016-04-25