[1]赵建伍　于耀宇　余天垒　李延良　唐　铸　桂　铮　周　欣　姬文婕.黄芪甲苷对大鼠蛛网膜下腔出血后脑血管痉挛的影响[J].中国临床神经外科杂志,2015,(06):350-352.[doi:10.13798/j.issn.1009-153X.2015.06.010]
　ZHAO Jian-wu,YU Yao-yu,YU Tian-lei,et al.Effects of Astragaloside Ⅳ on delayed cerebral vasospasm after subarachnoid hemorrhage[J].,2015,(06):350-352.[doi:10.13798/j.issn.1009-153X.2015.06.010]
点击复制

黄芪甲苷对大鼠蛛网膜下腔出血后脑血管痉挛的影响()
分享到：

《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2015年06期
页码:
350-352
栏目:
论著
出版日期:
2015-06-30

文章信息/Info

Title:
Effects of Astragaloside Ⅳ on delayed cerebral vasospasm after subarachnoid hemorrhage
文章编号:
1009-153X（2015）06-0350-03
作者:
赵建伍　于耀宇　余天垒　李延良　唐　铸　桂　铮　周　欣　姬文婕
121000 锦州，辽宁医学院研究生学院（赵建伍）；300162 天津，武警后勤学院附属医院神经外科（于耀宇、余天垒、李延良、唐　铸、桂　铮、周　欣、姬文婕）
通讯作者：于耀宇，E-mail：yuyaoyu666@aliyun.com
Author(s):
ZHAO Jian-wu1;  YU Yao-yu2;  YU Tian-lei2;  LI Yan-liang2;  TANG Zhu2;  GUI Zheng2;  ZHOU Xin2;  JI Wen-jie2.
1. Graduate School, Liaoning Medical College, Jinzhou 121000, China; 2. Department of Neurosurgery, Affiliated Hospital, Logistics University, Chinese People's Armed Police Forces, Tianjin 300162, China
关键词:
蛛网膜下腔出血; 脑血管痉挛; Toll样受体4; 核转录因子-κB; 黄芪甲苷; 大鼠
Keywords:
Astragaloside ;  Subarachnoid hemorrhage;  Delayed cerebral vasospasm ;  TLR4;  NF-κBⅣ
分类号:
R 743.9
DOI:
10.13798/j.issn.1009-153X.2015.06.010
文献标志码:
A
摘要:
目的 探讨黄芪甲苷对大鼠蛛网膜下腔出血（SAH）后脑血管痉挛（CVS）的作用。方法 将成年雄性SD大鼠40只随机分为正常组、SAH组、二甲基亚砜组（DMSO，腹腔注射等体积0.1% DMSO）和黄芪甲苷组（腹腔注射用0.1% DMSO助溶的黄芪甲苷溶悬液，2 mg/ml），每组10只。采用血管内刺破法制作SAH模型。HE染色观察基底动脉形态改变；免疫组化染色分析基底动脉Toll样受体4（TLR4）、核转录因子kappa B（NF-κB）的表达。结果 与正常组相比，SAH组和DMSO组基底动脉管壁明显增厚（P<0.05），而管腔横截面积明显缩小（P<0.05）；SAH组和DMSO组无明显差异（P>0.05）。黄芪甲苷组基底动脉管壁厚度较DMSO组明显变薄（P<0.05），管腔横截面积较DMSO组明显增大（P<0.05）。与正常组相比，SAH组和DMSO组基底动脉TLR4、NF-κB阳性率明显增高（P<0.05），而SAH组和DMSO组无明显差异（P>0.05）。黄芪甲苷组基底动脉TLR4、NF-κB阳性率较DMSO组明显下降（P<0.05）。结论 黄芪甲苷可能通过干预TLR4、NF-κB介导的炎症信号通路来缓解大鼠SAH后CVS。
Abstract:
Objective To explore the effect of Astragaloside Ⅳ(AS-Ⅳ) on delayed cerebral vasospasm after subarachnoid hemorrhage (SAH) and its mechanism. Methods Forty male SD rats were randomly were divided into 4 groups of 10 animals each, i.e. normal, SAH+AS-Ⅳ, SAH+ dimothyl sulfoxide (DMSO) and SAH groups. The SAH model was made by endovascularly puncturing the internal carotid artery. The rats were given daily by intraperitoneal injection of AS-Ⅳsuspension in SAH+AS-Ⅳ group 30 minutes after the establishment of the model, equal volume of 0.1% DMSO in SAH+DMSO group, and equal volume of saline in the normal and SAH groups. The morphological changes in the basilar arteries were observed by a microscope, the expressions of TLR4 and NF-κB p65 in the basilar arteries were determined by immunohistochemical technique, and the plasma levels of TNF-α and IL-6 were determined by enzyme-linked immunosorbent assay. Results The thickness of the basilar artery wall was significantly thinner in the normal group than that in SAH+AS-Ⅳgroup (P<0 .05), which was significantly thinner than that those in SAH and SAH+DMSO groups (P<0.05). The area of transverse section of the basilar artery was significantly bigger in the normal group than that in SAH+AS-Ⅳ group (P<0.05), which was significantly bigger than those in SAH and SAH+DMSO groups (P<0.05). The levels of TLR4 and NF-κB p65 expressions in the basilar arteries were significantly lower in the normal group than those in SAH+AS-Ⅳ group (P<0.05), which were significantly lower in SAH and SAH+DMSO groups (P<0.05). The plasma levels of TNF-α and IL-6 were significantly lower in the normal group than those in SAH+AS-Ⅳ group (P<0.05), which were significantly lower in SAH and SAH+DMSO groups (P<0.05). Conclusion AS-Ⅳ can alleviate the delayed cerebral vasospasm after SAH via inhibiting TLR4 and NF-κB-mediated inflammatory signaling pathways.

参考文献/References:

[1] Yang J, Wang HX, Zhang YJ, et al. Astragaloside Ⅳ atte- nuates inflammatory cytokines by inhibiting TLR4/NF-к B signaling pathway in isoproterenol-induced myocardial hypertrophy [J]. J Ethnopharmacol, 2013, 150(3): 1062- 1070.
[2] Gules I, Satoh M, Clower BR, et al. Comparison of three rat models of cerebral vasospasm [J]. Am J Physiol Heart Circ Physiol, 2002, 283(6): 2551-2559.
[3] Bederson JB, Connolly ES, Batjer HH, et al. Guidelines for the management of aneurysmal subarachnoid hemorrhage a statement for healthcare professionals from a special Writing Group of the Stroke Council, American Heart Association [J]. Stroke, 2009, 40(3): 994-1025.
[4] Dorhout Mees SM, Rinkel GJ, Feigin VL, et al. Calcium antagonists for aneurysmal subarachnoid haemorrhage [J]. Cochrane Database Syst Rev, 2007, 3.
[5] Wu Y, Zhao XD, Zhuang Z, et al. Peroxisome proliferator- activated receptor gamma agonist rosiglitazone attenuates oxyhemoglobin-induced Toll-like receptor 4 expression in vascular smooth muscle cells [J]. Brain Res, 2010, 1322: 102-108.
[6] Zhou M, Wu W, Ding Y, et al. Expression of Toll-like receptor 4 in the basilar artery after experimental subarach- noid hemorrhage in rabbits: a preliminary study [J]. Brain Res, 2007, 1173: 110-116.
[7] Lin S, Yin Q, Zhong Q, et al. Heme activates TLR4- mediated inflammatory injury via MyD88/TRIF signaling pathway in intracerebral hemorrhage [J]. J Neuroinflamma- tion, 2012, 9(1): 46-46.
[8] Ren S, Zhang H, Mu Y, et al. Pharmacological effects of Astragaloside Ⅳ: a literature review [J]. J Tradit Chin Med, 2013, 33(3): 413-416.
[9] Li M, Ma RN, Li LH, et al. Astragaloside Ⅳ reduces cere- bral edema post-ischemia/reperfusion correlating the sup- pression of MMP-9 and AQP4 [J]. Eur J Pharmacol, 2013, 715(1): 189-195.
[10] Li M, Qu YZ, Zhao ZW, et al. Astragaloside Ⅳ protects against focal cerebral ischemia/reperfusion injury correla- ting to suppression of neutrophils adhesion-related molecu- les [J]. Neurochem Int, 2012, 60(5): 458-465.

相似文献/References:

[1]周路桥　罗富强　彭　虎　邱成元　刘　苏　蒋国辉　田　强　罗荣武.皮瓣塌陷综合征患者颅骨修补术后继发蛛网膜下腔出血1例[J].中国临床神经外科杂志,2015,(11):704.[doi:10.13798/j.issn.1009-153X.2015.11.022]
[2]余龙洋　李亚楠　周　宇　戴冬伟　曹依群　岳志健.垂体腺瘤经蝶术后并发蛛网膜下腔出血的临床分析 （附6例报道）[J].中国临床神经外科杂志,2016,(06):372.[doi:10.13798/j.issn.1009-153X.2016.06.018]
[3]周　量　刘　丛　张昌伟.早期栓塞术治疗动脉瘤性蛛网膜下腔出血的疗效[J].中国临床神经外科杂志,2016,(07):410.[doi:10.13798/j.issn.1009-153X.2016.07.007]
　ZHOU Liang,LIU Cong,ZHANG Chang-wei..Curative effect of early endovascular embolization on patients with ruptured intracranial aneurysms[J].,2016,(06):410.[doi:10.13798/j.issn.1009-153X.2016.07.007]
[4]朱作磊 综述　罗良生 审校.动脉瘤性蛛网膜下腔出血的预后相关因素研究进展[J].中国临床神经外科杂志,2016,(03):183.[doi:10.13798/j.issn.1009-153X.2016.03.020]
[5]廖驭国　刘胜初　钟云天　罗　捷.腰大池引流术防治颅内动脉瘤栓塞术后脑血管痉挛的临床疗效[J].中国临床神经外科杂志,2016,(02):89.[doi:10.13798/j.issn.1009-153X.2016.02.010]
　LIAO Yu-guo,LIU Sheng-chu,CHU Yun-tian,et al.Effect of lumbar cistern drainage on cerebral vasospasm in patients with ruptured intracranial aneurysms after embolization[J].,2016,(06):89.[doi:10.13798/j.issn.1009-153X.2016.02.010]
[6]邹志鹏　杨　海　夏军勇　张化明　张　华　魏　恒　马江红.行血管内治疗的高龄颅内动脉瘤患者脑血管痉挛的临床分析[J].中国临床神经外科杂志,2015,(07):401.[doi:10.13798/j.issn.1009-153X.2015.07.006]
　ZOU Zhi-peng,YANG Hai,XIA Jun-yong,et al.Clinical analysis of symptomatic cerebral vasospasm after endovascular treatment of ruptured cerebral aneurysms in patients of 70 and over 70 years[J].,2015,(06):401.[doi:10.13798/j.issn.1009-153X.2015.07.006]
[7]祝　源　李　俊　陈　刚　向伟楚　李国栋　赵曰圆　李欢欢　汤　伟　金二亮　马廉亭.两次DSA阴性的颅内破裂动脉瘤1例报道并文献复习[J].中国临床神经外科杂志,2015,(09):538.[doi:10.13798/j.issn.1009-153X.2015.09.009]
　ZHU Yuan,LI Jun,CHEN Gang,et al.Both negative DSA findings in 1 patient with ruptured intracranial aneurysm (case report and review of literature)[J].,2015,(06):538.[doi:10.13798/j.issn.1009-153X.2015.09.009]
[8]陈　旭　吴　琳　冯达云　王举磊　秦怀洲　高国栋　张治国.头孢曲松钠对大鼠蛛网膜下腔出血后认知功能的影响[J].中国临床神经外科杂志,2015,(04):228.[doi:10.13798/j.issn.1009-153X.2015.04.012]
　CHEN Xu,WU Lin,FENG Da-yun,et al.Effect of ceftriaxone on cognitive function after subarachnoid hemorrhage in the adult rats[J].,2015,(06):228.[doi:10.13798/j.issn.1009-153X.2015.04.012]
[9]刘骏辉 综述　陈谦学 审校.动脉瘤性蛛网膜下腔出血后抗血管痉挛的药物治疗[J].中国临床神经外科杂志,2016,(08):504.[doi:10.13798/j.issn.1009-153X.2016.08.024]
[10]郎胜坤　许厚银　董月青　马铁柱　孙　艳　郝芊芊　王慧敏.磁共振灌注成像评估蛛网膜下腔出血后脑血管痉挛的价值[J].中国临床神经外科杂志,2016,(09):517.[doi:10.13798/j.issn.1009-153X.2016.09.002]
　LANG Sheng-kuan,XU Hou-yin,DONG Yue-qing,et al.Evaluation of cerebral vasospasm after aneurysmal subarachnoid hemorrhage by magnetic resonance perfusion weighted imaging[J].,2016,(06):517.[doi:10.13798/j.issn.1009-153X.2016.09.002]
[11]旷仁钊　唐晓平　漆　建　苟章洋.丹红注射液对大鼠蛛网膜下腔出血后脑血管痉挛的影响[J].中国临床神经外科杂志,2015,(11):683.[doi:10.13798/j.issn.1009-153X.2015.11.014]
　KUANG Ren-zhao,TANG Xiao-ping,QI Jian,et al.Effects of Danhong on cerebral vasospasm after subara- chnoid hemorrhage in rats[J].,2015,(06):683.[doi:10.13798/j.issn.1009-153X.2015.11.014]
[12]李朝晖　王玉贵　张孟超 综述　赵兴利 审校.CT灌注成像在动脉瘤性蛛网膜下腔出血中的 临床应用进展[J].中国临床神经外科杂志,2016,(05):313.[doi:10.13798/j.issn.1009-153X.2016.05.021]
[13]水　涛　郭再玉 综述　马廉亭 审校.蛛网膜下腔出血后脑血管痉挛的亚低温治疗进展[J].中国临床神经外科杂志,2016,(03):185.[doi:10.13798/j.issn.1009-153X.2016.03.021]
[14]旷仁钊 综述　唐晓平 审校.蛛网膜下腔出血后脑血管痉挛药物治疗的研究进展[J].中国临床神经外科杂志,2015,(09):572.[doi:10.13798/j.issn.1009-153X.2015.09.024]
[15]赵乐,综述,曹毅,等.尼莫地平在aSAH治疗中的研究进展[J].中国临床神经外科杂志,2017,(02):115.[doi:10.13798/j.issn.1009-153X.2017.02.020]
[16]熊　平　唐晓平.促红细胞生成素对大鼠蛛网膜下腔出血后血管痉挛的防治作用[J].中国临床神经外科杂志,2018,(04):254.[doi:10.13798/j.issn.1009-153X.2018.04.010]
　XIONG Ping,TANG Xiao-ping..Preventive and therapeutic effects of erythropoietin on cerebral vasospasm after subarachnoid hemorrhage in rats[J].,2018,(06):254.[doi:10.13798/j.issn.1009-153X.2018.04.010]
[17]陈华轩　张　渊　王　林　宋旭东　刘　毅　范润金.缺血修饰白蛋白对动脉瘤性蛛网膜下腔出血后脑血管痉挛的评估价值[J].中国临床神经外科杂志,2019,(01):26.[doi:10.13798/j.issn.1009-153X.2019.01.007]
　CHEN Hua-xuan,ZHANG Yuan,WANG Lin,et al.Value of ischemia-modified albumin to assesment of cerebral vasospasm after aneurysmal subarachnoid hemorrhage[J].,2019,(06):26.[doi:10.13798/j.issn.1009-153X.2019.01.007]
[18]黄昌恒　于耀宇　赵文可　何一川　姚自同.黄芪甲苷对大鼠蛛网膜下腔出血后迟发性脑血管痉挛的影响[J].中国临床神经外科杂志,2019,(02):98.[doi:10.13798/j.issn.1009-153X.2019.02.011]
　HUANG Chang-heng,YU Yao-yu,ZHAO Wen-ke,et al.Effect of astragaloside A on delayed cerebral vasospasm in rats after subarachnoid hemorrhage[J].,2019,(06):98.[doi:10.13798/j.issn.1009-153X.2019.02.011]
[19]谭占国　黄圣明.蛛网膜下腔出血后迟发脑血管痉挛诊治新进展[J].中国临床神经外科杂志,2019,(08):505.[doi:10.13798/j.issn.1009-153X.2019.08.020]
[20]谢昌纪　周志宇　韦成聪.颅内动脉瘤破裂出血后脑血管痉挛发病机制的研究进展[J].中国临床神经外科杂志,2021,26(09):727.[doi:10.13798/j.issn.1009-153X.2021.09.026]

更新日期/Last Update: 2015-06-30