[1]郑　波　陈　涛　毛　华.CpG-ODN对大鼠脑缺血/再灌注损伤的保护作用[J].中国临床神经外科杂志,2018,(03):176-181.[doi:10.13798/j.issn.1009-153X.2018.03.012]
　ZHENG Bo,CHEN Tao,MAO Hua..Effect of OCpG-ODN on cerebral tissues injured by cerebral ischemia/reperfusion and JAK and STAT expressions in rats[J].,2018,(03):176-181.[doi:10.13798/j.issn.1009-153X.2018.03.012]
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CpG-ODN对大鼠脑缺血/再灌注损伤的保护作用()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2018年03期
页码:
176-181
栏目:
论著
出版日期:
2018-04-01

文章信息/Info

Title:
Effect of OCpG-ODN on cerebral tissues injured by cerebral ischemia/reperfusion and JAK and STAT expressions in rats
文章编号:
1009-153X（2018）03-0176-06
作者:
郑　波　陈　涛　毛　华
作者单位：434100 湖北，荆州市中心医院神经外科（郑　波、陈　涛、毛　华）
Author(s):
ZHENG Bo;  CHEN Tao;  MAO Hua.
Department of Neurosurgery, Jingzhou Central Hospital, Jingzhou 434100, China
关键词:
脑缺血/再灌注损伤; CpG-ODN; 细胞凋亡; JAK; STAT; 大鼠
Keywords:
Cerebral ischemia/reperfusion injury;  CpG-oligodeoxynucleotide;  Apoptosis;  Janus Kinase;  Signal transducer and activator of transcription;  Neuroprotective effects
分类号:
R 743
DOI:
10.13798/j.issn.1009-153X.2018.03.012
文献标志码:
A
摘要:
目的 探讨CpG-ODN对大鼠脑缺血/再灌注（I/R）损伤的保护作用及其机制。方法 将54只大鼠随机分为假手术组、脑I/R模型组、CpG-ODN组，每组18只。采用大脑中动脉线栓法构建大鼠脑I/R模型。CpG-ODN组造模前1 h腹腔注射CpG-ODN（10 μg/25 g），脑I/R模型组注射等量生理盐水。再灌注12 h采用Longa 5分评分法评估神经功能。再灌注24 h采用TTC染色测定脑梗死体积，HE染色观察组织结构，TUNEL染色分析细胞凋亡，PCR检测酪氨酸激酶（JAK）、信号转导和转录激活因子（STAT）mRNA表达水平，免疫印迹法分析Caspase3、Caspase7、JAK、p-JAK、STAT和p-STAT蛋白水平。结果 与假手术组比较，脑I/R模型组神经功能障碍严重，脑梗死体积显著增大，脑组织发生水肿、坏死等病变；与脑I/R模型组比较，CpG-ODN组明显改善。与假手术组比较，脑I/R模型组脑组织细胞凋亡率和Caspase3、Caspase7蛋白水平明显升高（P<0.05），脑I/R模型组JAK、STAT mRNA水平和蛋白磷酸化程度显著上调（P<0.05）；与脑I/R模型组比较，CpG-ODN组细胞凋亡率和Caspase3、Caspase7蛋白水平明显降低（P<0.05），JAK、STAT mRNA和蛋白磷酸化程度均明显下降（P<0.05）。结论 CpG-ODN能有效保护大鼠脑I/R损伤，其机制可能与影响JAK、STAT蛋白磷酸化抑制细胞凋亡有关。
Abstract:
Objective To explore protective effect of oCpG-ODN on cerebral tissues injured by cerebral ischemia/reperfusion (I/R) and the expressions of Janus kinase (JAK) and signal transduction and activator of transcription (STAT) in rats. Methods Brain I/R model was constructed by middle cerebral artery occlusion (MCAO) method. Fifty-four SD rats were randomly divided into three groups of 18 animals each, i.e. sham operation group, brain I/R group, and CpG-oligodeoxynucleotide (CpG-ODN) treatment group, in which 10 μg/25g CpG-ODN was intraperitonealy injected before the MCAO. Neurological function, cerebral infarction volume and morphological changes in the cerebral tissues were determined. The cells apoptosis and JAK and STAT mRNA levels and JAK, phosphorylated JAK, p-JAK, STAT, p-STAT and Caspase 3 and 7 proteins expressions in the infarct cerebral tissues were detected 24 hours after I/R. Results The cerebral dysfunction was significantly more severe and the volumes of the cerebral infarction was significantly bigger in the I/R group than those in CpG-ODN treatment group (P<0.05), which were significantly more severe and bigger than those in the sham operation group (P<0.05). The cell apoptosis rate, Caspase 3 and 7 proteins levels, the expression levels of mRNA of STA and JAK, and the levels of p-STA and p-JAK protein expressions were significantly higher in I/R group than those in the sham operation group and CpG-ODN treatment group. The levels of p-STA and p-JAK proteins expressions were significantly higher in CpG-ODN treatment group than those in the sham operation group (P<0.05). Conclusion It is suggested that CpG-ODN can effectively protect injured cerebral tissues after I/R possibly by promoting JAK and STAT protein phosphorylation and inhabiting cell apoptosis.

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更新日期/Last Update: 2018-05-05