[1]夏 星,徐治强,彭 翔.姜黄素对APP/PS1双转基因小鼠认知功能、炎症反应及海马区突触素表达的影响[J].中国临床神经外科杂志,2018,(09):609-612.[doi:10.13798/j.issn.1009-153X.2018.09.011]
　XIA Xing,XU Zhi-qiang,PENG Xiang..Effect of curcumin on SYP expression in hippocampal CA1 area, inflammatory response and cognitive function in APP/PS1 double transgenic mice[J].,2018,(09):609-612.[doi:10.13798/j.issn.1009-153X.2018.09.011]
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姜黄素对APP/PS1双转基因小鼠认知功能、炎症反应及海马区突触素表达的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2018年09期
页码:
609-612
栏目:
论著
出版日期:
2018-09-25

文章信息/Info

Title:
Effect of curcumin on SYP expression in hippocampal CA1 area, inflammatory response and cognitive function in APP/PS1 double transgenic mice
文章编号:
1009-153X（2018）09-0609-04
作者:
夏 星; 徐治强; 彭 翔
430034，武汉市第四医院西院区神经外科（夏 星、徐治强、彭 翔）
Author(s):
XIA Xing;  XU Zhi-qiang;  PENG Xiang.
Department of Neurosurgery, The Fourth Hospital of Wuhan, Wuhan 430000, China
关键词:
姜黄素; APP/PS1双转基因小鼠; 炎性反应; 突触素; 认知功能
Keywords:
Curcumin;  APP/PS1 double transgenic mice;  Inflammatory response;  Synatophysin;  Cognitive function
分类号:
R 742
DOI:
10.13798/j.issn.1009-153X.2018.09.011
文献标志码:
A
摘要:
目的 探讨姜黄素对APP/PS1双转基因小鼠认知功能、炎症反应及海马区突触素表达的影响。方法 60只6月龄APP/PS1双转基因雄性小鼠随机分为A组、B组和C组，各20只；A组采用姜黄素100mg/kg/d加入小鼠饲料喂养，B组采用姜黄素300mg/kg/d喂养，C组采用姜黄素600mg/kg/d喂养；3组均喂养6个月。治疗前、治疗3、6个月，采用Morris水迷宫实验评估小鼠认知功能，采用免疫吸附试验法检测尾静脉血血清白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）等炎症因子水平；治疗6个月，采用免疫组化染色法检测小鼠海马 CA1 区突触素表达情况。结果 姜黄素治疗3、6个月，3组小鼠认知功能明显改善（P<0.05），血清IL-6、TNF-α水平明显降低（P<0.05）。与A组比较，B组和C组治疗3、6个月认知功能明显改善（P<0.05），血清IL-6、TNF-α水平均明显降低（P<0.05），海马CA1区突触素表达水平明显升高（P<0.05）。而B组与C组均无统计学差异（P>0.05）。结论 姜黄素有助于改善APP/PS1双转基因小鼠海马突触素表达，抑制小鼠炎症反应，改善小鼠认知功能。
Abstract:
Objective To investigate the effects of curcumin on synatophysin (SYP) expression in hippocampals CA1 area, the inflammatory response and cognitive function in APP/PS1 double transgenic mice. Methods Sixty APP/PS1 double transgenic mice aged 6 months were randomly divided into three groups, i.e. group A in which 20 mice were fed with 100 mg/kg/d curcumin for six months, group B in which 20 mice were fed with 300 mg/kg/d curcumin for six months, and group C in which 20 mice were fed with 600 mg/kg/d curcumin. The cognitive function was determined by Morris water maze test and The levels of serum interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) were determined by enzyme linked immunosorbent assay before the treatment and 3 and 6 months after the treatment in all the mice. SYP expression in hippocampal CA1 area was detected by immuinohistochemical technique 6 months after the treatment in all the mice. Results The number of passing through the platform and time of staying in the target quadrant were significantly more 3 and 6 months after the treatment respectively than those before the treatment in all the groups (P<0.05). Three and six months after the treatment, the number of passing through the platform and time of staying in the target quadrant were significantly more in groups B and C respectively than those in group A (P<0.05). The levels of serum IL-6 and TNF-α were significantly lower 3 and 6 months after the treatment respectively than those before the treatment in all the groups (P<0.05). Three and six months after the treatment, the levels of serum IL-6 and TNF-αwere significantly lower in groups C and B respectively than those in group A (P<0.05). The positive rates of SYP expression in the hippocanpal CA1 area were significantly higher in groups B and C respectively than that in group A 6 months after the treatment (P<0.05). Conclusion Curcumin can improve SYP expression in hippocampal CA1 area, the inflammatory response and cognitive function to a certain extent in dose-and time- dependent form in the APP/PS1 double transgenic mice.

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备注/Memo

备注/Memo:
基金项目：武汉市临床医学科研基金（WZ17Z07）
更新日期/Last Update: 2018-09-30