[1]杨建龙　葛晗明　闫忠军　胡　焱.lncRNA-ADAMTS9-AS2与胶质瘤预后的相关性[J].中国临床神经外科杂志,2019,(05):276-278.[doi:10.13798/j.issn.1009-153X.2019.05.006]
　YANG Jian-long,GE Han-ming,YAN Zhong-jun,et al.Correlativity between low expression level of long non-coding RNA ADAMTS9-AS2 and poor prognosis in patients with gliomas[J].,2019,(05):276-278.[doi:10.13798/j.issn.1009-153X.2019.05.006]
点击复制

lncRNA-ADAMTS9-AS2与胶质瘤预后的相关性()
分享到：

《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2019年05期
页码:
276-278
栏目:
论著
出版日期:
2019-05-27

文章信息/Info

Title:
Correlativity between low expression level of long non-coding RNA ADAMTS9-AS2 and poor prognosis in patients with gliomas
文章编号:
1009-153X（2019）05-0276-03
作者:
杨建龙　葛晗明　闫忠军　胡　焱
710038 西安，空军军医大学第二附属医院神经外科[杨建龙[现在陕西省榆林市第一医院神经外科工作）、闫忠军、胡　焱]；710000 西安，西北大学附属医院/西安市第三医院神经内科（葛晗明）
Author(s):
YANG Jian-long1; 2;  GE Han-ming3;  YAN Zhong-jun1;  HU Yan1
1. Department of Neurosurgery, The Second Affiliated Hospital, Air Force Military Medical University, Xi’an 710038, China; 2. Department of Neurosurgery, Yulin Municipal Hospital, Yulin 718000, China; 3. Department of Neurology, The Affiliated Xi’an Hospital, Northwest University, Xi’an 710000, China
关键词:
神经胶质瘤; 长链非编码RNA-ADAMTS9-AS2; 临床预后; 肿瘤标志物
Keywords:
Gliomas;  lncRNA-ADAMTS9-AS2;  Clinical prognosis;  Tumor markers
分类号:
R 739.41; Q 786
DOI:
10.13798/j.issn.1009-153X.2019.05.006
文献标志码:
A
摘要:
目的 探讨长链非编码RNA-ADAMTS9-AS2（lncRNA-ADAMTS9-AS2）在神经胶质瘤组织中的表达情况及其与胶质瘤病人预后的相关性。方法 收集2009年6月至2013年6月手术治疗的原发性神经胶质瘤57例（WHO Ⅰ~Ⅱ级27例，Ⅲ~Ⅳ级30例）。应用qRT-PCR法分别检测神经胶质瘤组织以及瘤旁正常脑组织lncRNA-ADAMTS9-AS2的表达水平。所有入选病人术后均进行5年随访或直到死亡，根据Kaplan-Meier方法进行生存曲线分析，采用多因素 Cox比例风险回归模型分析死亡的风险因素。结果 与瘤旁正常脑组织相比，胶质瘤组织lncRNA-ADAMTS9-AS2的表达水平明显降低（P<0.001）；而且，高级别（WHO Ⅲ~Ⅳ级）胶质瘤表达水平明显低于低级别（WHOⅠ~Ⅱ级）胶质瘤（P<0.05）。57例中，lncRNA-ADAMTS9-AS2呈低表达31例（低表达组），高表达26例（高表达组）。57例中，死亡30例，其中低表达组21例（67.7%），高表达组10例（38.5%）。生存曲线分析显示，低表达组病人生存时间较高表达组明显缩短（P<0.05）。多因素 Cox比例风险回归模型分析结果显示，lncRNA-ADAMTS9-AS2低表达是预测胶质瘤病人预后不良的独立风险因素（HR=2.413；95% CI 1.462~4.421；P=0.004）。结论 lncRNA-ADAMTS9-AS2在神经胶质瘤中表达下调，其可作为胶质瘤病人预后不良的肿瘤标志物。
Abstract:
ObjectiveToinvestigatetheexpressionoflongnon-codingRNAADAMTS9-AS2(lncRNA-ADAMTS9-AS2)ingliomatissuesinordertoexplorethecorrelativityofitsexpressionlevelwiththeclinicalprognosisinthepatientswithgliomas.MethodsThelevelsofADAMTS9-AS2expressionin57casesofgliomatissuesofwhich,11belongedinWHOgradeⅠ16ingradeⅡ,22ingradeⅢand8ingradeⅣ,and57casesofperitumorousnormalbraintissuesweredetectedbyqRT-PCR,andthentherelationshipoftheexpressionlevelofADAMTS9-AS2withtheclinicopathologicalfactorsandprognosisinthepatientswithgliomaswasstatisticallyanalyzed.ResultsThelevelofADAMTS9-AS2expressionwassignificantlylowerinthegliomatissuesthanthatinthenormalbraintissues(P<0.05).ThelevelofADAMTS9-AS2expressionwassignificantlylowerinWHOgradesⅢ~ⅣgliomasthanthatinWHOgradesⅠ~Ⅱ(P<0.01).ThelevelofADAMTS9-AS2expressionwassignificantlyhigherinthegliomastissuesderivedfromthepatientswithKarnofskyperformancescale(KPS)≥80orwithoutthepostoperativerecurrentgliomasthanthoseinthegliomastissuesderivedfromthepatientswith<80scoresorthepatientssufferingfromtherecurrentgliomasafterthesurgery(P<0.05).ConclusionsThisstudysuggestedthatthedown-regulationofADAMTS9-AS2expressioninthegliomatissuesmayplayaimportantroleintheathogenesisanddevelopmentofgliomas,anditcanbeusedasamarkerofpoorprognosisinthepatientswithgliomas.

参考文献/References:


[1] 林婷婷，李　钢. 脑胶质瘤的综合临床治疗的研究进展[J]. 中国临床神经外科杂志，2013，18（5）：316-319.
[2] 赛　克，陈忠平. 胶质瘤的疗效评价[J]. 中国临床神经外科杂志，2016，21（6）：321-322.
[3] Dianatpour A, Ghafouri-Fard S. Long non coding RNA expression intersecting cancer and spermatogenesis: a systematic review [J]. Asian Pac J Cancer Prev, 2017, 18(10): 2601-2610.
[4] Kraus TF, Greiner A, Guibourt V, et al. Identification of stably expressed lncrnas as valid endogenous controls for profiling of human glioma [J]. J Cancer, 2015, 6(2): 111-119.
[5] Barry G. Integrating the roles of long and small non-coding RNA in brain function and disease [J]. Mol Psychiatry, 2014, 19(4): 410-416.
[6] Koch L. Functional genomics: screening for lncRNA func-tion [J]. Nat Rev Genet, 2017, 18(2): 70.
[7] 张素洁，胡 　毅，马俊勋. 长链非编码RNA检测在肿瘤诊断及预后评估中的应用综述[J]. 解放军医学院学报，2016，37（8）：912-915.
[8] Li Y, Zhu G, Zeng W, et al. Long noncoding RNA AB073614 promotes the malignance of glioma by activating Wnt/beta-catenin signaling through downregulating SOX7 [J]. Oncotarget, 2017, 8(39): 65577-65587.
[9] Liu G, Zhao X, Zhou J, et al. LncRNA TP73-AS1 Promotes cell proliferation and inhibits cell apoptosis in clear cell renal cell carcinoma through repressing kiss1 expression and inactivation of PI3K/Akt/mTOR signaling pathway [J]. Cell Physiol Biochem, 2018, 48(1): 371-384.
[10] Wang Q, Zhang J, Liu Y, et al. A novel cell cycle-associa-ted lncRNA, HOXA11-AS, is transcribed from the 5-prime end of the HOXA transcript and is a biomarker of progression in glioma [J]. Cancer Lett, 2016, 373(2): 251-259.
[11] Liu C, Yang Z, Deng Z, et al. Upregulated lncRNA ADAMTS9-AS2 suppresses progression of lung cancer through inhibition of miR-223-3p and promotion of TGFBR3 [J]. Iubmb Life, 2018, 70(6):536-546.
[12] Yao J, Zhou B, Zhang J, et al. A new tumor suppressor lnc-RNA ADAMTS9-AS2 is regulated by DNMT1 and inhibitsmigration of glioma cells [J]. Tumour Biol, 2014, 35(8): 7935-7944.

备注/Memo

备注/Memo:

通讯作者：胡　焱，E-mail：yienhoo2008@163.com
更新日期/Last Update: 2019-05-27