[1]佘德源,唐 跃,黄 钦,等.脑皮层扩散性抑制对大鼠蛛网膜下腔出血后皮层微循环的影响[J].中国临床神经外科杂志,2019,(06):352-355.[doi:10.13798/j.issn.1009-153X.2019.06.011]
　SHE DE-yuan,TANG Yue,et al.Observation of effects of cortical spreading depression on cortical microcirculation after subarachnoid hemorrhage under transparent cranial window[J].,2019,(06):352-355.[doi:10.13798/j.issn.1009-153X.2019.06.011]
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脑皮层扩散性抑制对大鼠蛛网膜下腔出血后皮层微循环的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2019年06期
页码:
352-355
栏目:
论著
出版日期:
2019-06-25

文章信息/Info

Title:
Observation of effects of cortical spreading depression on cortical microcirculation after subarachnoid hemorrhage under transparent cranial window
文章编号:
1009-153X（2019）06-0352-04
作者:
佘德源; 唐 跃; 黄 钦; 温健鹏; 刘 鹏
430065 武汉，武汉科技大学医学院（佘德源、刘 鹏）；430070 武汉，中国人民解放军中部战区总医院神经外科（佘德源、唐 跃、温健鹏、刘 鹏）；430074 武汉，武汉国家光电实验室（黄 钦）
Author(s):
SHE DE-yuan1;  2;  TANG Yue2;  HUANG Qin3;  WEN Jian-peng2;  LIU Peng1;  2.
1. School of Medicine, Wuhan University of Sciences and Technology, Wuhan 430065, China; 2. Department of Neurosurgery, General Hospital, Central Theatre, PLA, Wuhan 430070, China; 3. Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China
关键词:
蛛网膜下腔出血; 皮层扩散性抑制; 激光散斑技术; 内源信号成像技术; 小鼠
Keywords:
Subarachnoid hemorrhage;  Cortical spreading depression;  Laser speckle technology;  Endogenous signal imaging;  Cerebral blood flow
分类号:
R 743.9
DOI:
10.13798/j.issn.1009-153X.2019.06.011
文献标志码:
A
摘要:
目的 探讨脑皮层扩散性抑制（CSD）对蛛网膜下腔出血（SAH）模型小鼠脑皮层微循环的影响。方法 将30只雄性成年C57小鼠随机分为正常组（n=10，制作透明颅窗、诱导窗加KCl）、对照组（n=10，制作透明颅窗及SAH模型、诱导窗加NaCl）和观察组（n=10，制作透明颅窗及SAH模型、诱导窗滴KCl）。采用颈动脉内穿刺法构建SAH模型。使用激光散斑血流成像和内源信号成像技术记录脑皮层微循环和CSD。结果 观察组、正常组出现CSD，对照组未产生CSD。观察组CSD后脑皮层血流明显下降（P<0.05）、血管明显收缩（P<0.05），其中CSD后5 d较显著。正常组CSD后血管明显舒张（P<0.05）、皮层血流明显增加（P<0.05）。对照组无明显变化（P>0.05）。结论 KCl能诱导产生CSD；CSD能使正常小鼠脑皮层血流增加和血管扩张；CSD使SAH后脑皮层血流减少和血管收缩。
Abstract:
Objective To observe the effects of cortical spreading depression (CSD) on the cortical microcirculation after subarachnoid hemorrhage (SAH). Methods Thirty C57 male mice were randomly divided into 3 groups of 10 animals each, i. e. normal group in which the transparent cranial windows were made and KCl dropped into the cranial window, control group in which the transparent cranial windows and SAH models were made and the physiological saline dropped into the window and experimental group in which the transparent cranial windows and SAH models were made and KCl dropped into the window. CSD and the cortical microcirculation was recorded by laser speckle blood flow imaging and endogenous signal imaging technology. Results ①CSD appeared in the experimental group and the normal group, but not in the control group. ②Compared to those before dropping the reagent, the cerebral cortical blood flow significantly decreased (P<0.05) and the cortical blood vessels significantly constricted, especially the 5th day after SAH in the experimental group (P<0.05), the cortical blood vessels significantly dilated (P<0.05) and the cortical blood flow significantly increased (P<0.05) in the normal group and the cortical blood vessels and blood flow were insignificantly changed in the control group (P>0.05) after dropping the reagent. Conclusions ①KCl can induce CSD in the normal and SAH mice cortexes. ②CSD can induce the increase in the cerebral blood flow and vasodilatation in normal mice cortexes. ③CSD can induce the decrease in the blood flow and vasoconstriction in the SAH mice cortexes.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金（81870945）；中国博士后科学基金（2018M641411）；湖北省卫生与计划生育委员会科研联合项目（WJ2018H0065） 通讯作者：刘 鹏，E-mail：5564793@qq.com
更新日期/Last Update: 2019-06-25