[1]张圣坤 综述 初 明 审校.重建椎管稳定性的颈椎椎板成形术的研究进展[J].中国临床神经外科杂志,2020,(07):488-490.[doi:10.13798/j.issn.1009-153X.2020.07.026]
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重建椎管稳定性的颈椎椎板成形术的研究进展()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2020年07期
页码:
488-490
栏目:
综述
出版日期:
2020-07-25

文章信息/Info

文章编号:
1009-153X(2020)07-0488-03
作者:
张圣坤 综述 初 明 审校
518000 广东,深圳市第三人民医院神经外科(张圣坤、初 明)
关键词:
脊柱手术脊柱稳定椎板成形术颈椎
分类号:
R 742.8
DOI:
10.13798/j.issn.1009-153X.2020.07.026
文献标志码:
A
Abstract:
Objective To explore the effect of overexpression of LRIG3 on the cell biological behaviors of human neuroblastoma cell line SK-N-MC. Methods Human neuroblastoma cell line SK-N-MC was cultured in vitro and then were randomly divided into three groups, i.e., control group (group A, did not transfect any vector virus), blank vector group (group B, transfected with blank vector virus) and LRIG3 overexpression group (group C, transfected with LRIG3 overexpression vector lentivirus). The expression levels of LRIG3, Caspase-3 and Caspase-9 were detected by RT-PCR and Western blot. Transwell kit was used to detect cell invasiveness, AV-PI kit was used to detect cell apoptosis and CCK-8 kit was used to detect cell proliferation. Results The expression levels of Caspase-3 and Caspase-9 in group C was significantly higher than those in groups A and B (P<0.05). The cell proliferation rate of group C was significantly lower than those of groups A and group B (P<0.05), and the cell invasion ability of group C was significantly lower than those of groups A and group B (P<0.05). The apoptotic rate in group C was significantly higher than those in groups A and B (P<0.05). Conclusion LRIG3 overexpression can inhibit the proliferation and invasion of human neuroblastoma cell line SK-N-MC, and promote the apoptosis of human neuroblastoma cell line SK-N-MC

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备注/Memo

备注/Memo:
(2018-11-05收稿,2018-12-07修回)通讯作者:初 明,E-mail:chuming120@163.com
更新日期/Last Update: 2020-07-20