[1]樊帅帅,宋磊军,蔺聪,等.下调lncRNA-MALAT1表达对新生大鼠缺氧缺血性脑损伤的保护作用[J].中国临床神经外科杂志,2022,27(05):376-379.[doi:10.13798/j.issn.1009-153X.2022.05.013]
　FAN Shuai-shuai,SONG Lei-jun,LIN Cong,et al.Protective effect of down-regulation of lncRNA-MALAT1 on hypoxic-ischemic brain damage in neonatal rats[J].,2022,27(05):376-379.[doi:10.13798/j.issn.1009-153X.2022.05.013]
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下调lncRNA-MALAT1表达对新生大鼠缺氧缺血性脑损伤的保护作用()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
27
期数:
2022年05期
页码:
376-379
栏目:
实验研究
出版日期:
2022-05-31

文章信息/Info

Title:
Protective effect of down-regulation of lncRNA-MALAT1 on hypoxic-ischemic brain damage in neonatal rats
文章编号:
1009-153X(2022)05-0376-04
作者:
樊帅帅; 宋磊军; 蔺聪; 刘扬
450000，郑州市第七人民医院麻醉科（樊帅帅、宋磊军、蔺聪）；450000郑州，中国人民解放军联勤保障部队第988医院麻醉科（刘扬）
Author(s):
FAN Shuai-shuai1;  SONG Lei-jun1;  LIN Cong1;  LIU Yang2
1.Department of Anesthesiology, Zhengzhou Seventh People's Hospital, Zhengzhou 450000, China; 2.Department of Anesthesiology, The 988 Hospital of The Joint Logistics Support Force, PLA, Zhengzhou 450000, China
关键词:
缺氧缺血性脑损伤; 新生大鼠; 神经元凋亡; 长链非编码RNA; lncRNA-MALAT1
Keywords:
Hypoxic-ischemic brain damage;  Neonatal rat;  Neuron apoptosis;  Long non-coding RNA;  lncRNA LAMAT1
分类号:
R743
DOI:
10.13798/j.issn.1009-153X.2022.05.013
文献标志码:
A
摘要:
目的 探讨小图lncRNA-MALAT1表达对新生大鼠缺氧缺血性脑损伤（HIBD）的影响。方法 取SPF级7 d龄SD大鼠40只，随机分为假手术组、模型组、lncRNA对照组、silncMALAT1组，每组10只。Rice-Vannucci法建立新生大鼠HIBD模型，造模后2 h，侧脑室分别注射生理盐水、生理盐水、空载体重组腺病毒液、silncMALAT1各5 μl。造模后7 d，Morris水迷宫试验评估空间学习和记忆能力，TUNEL染色检测海马组织神经元凋亡，PCR和免疫印迹法检测海马组织BDNF/TrkB信号通路mRNA和蛋白表达变化。结果 模型组造模失败2只，lncRNA组失败2只，silncMALAT1组失败1只，造模成功率为83.33%。下调lncRNA-MALAT1表达，明显增加新生大鼠学习和记忆能力（P<0.05），明显减少海马组织神经元凋亡率（P<0.05），明显下调BDNF/TrkB mRNA和蛋白表达水平（P<0.05）。结论 下调lncRNA-MALAT1表达可减轻新生大鼠HIBD，其机制可能与抑制BDNF/TrkB信号通路、减轻海马组织神经元凋亡有关。
Abstract:
Objective To investigate the effect of dowm-regulation of lncRNA-MALAT1 on the hypoxic-ischemic brain damage (HIBD) in neonatal rats. Methods Forty 7-day-old SPF SD rats were randomly divided into sham operation group, model group, lncRNA control group, and silncMALAT1 group, with 10 rats in each group. The HIBD model of neonatal rats was established by the Rice-Vannucci method. Two hours after operation, 5 μl of normal saline, normal saline, empty vector recombinant adenovirus solution, and silncMALAT1 were injected into the lateral ventricle of the rats in the four groups, respectively. Seven days after operation, spatial learning and memory abilities were evaluated by Morris water maze test, neuronal apoptosis in hippocampal tissues was detected by TUNEL staining, and changes in mRNA and protein expressions of BDNF/TrkB signaling pathway in hippocampal tissues were detected by PCR and Western blotting, respectively. Results There were 2 failures in the model group, 2 failures in the lncRNA group, and 1 failure in the silncMALAT1 group, with a success rate of model establishment of 83.33%. Down-regulation of lncRNA-MALAT1 significantly increased the learning and memory abilities of neonatal rats (P<0.05), significantly decreased the apoptosis rate of neurons in the hippocampal tissues (P<0.05), and significantly down-regulated the mRNA and protein expression levels of BDNF/TrkB (P<0.05). Conclusions Down-regulation of lncRNA-MALAT1 can alleviate HIBD in neonatal rats, and the mechanism may be related to inhibiting BDNF/TrkB signaling pathway and reducing neuronal apoptosis in hippocampal tissues.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：河南省科技攻关计划项目（LHGJ20190883）
更新日期/Last Update: 2022-06-30