[1]高剑峰　姚庆和　李晓辉　龙宇波　陈振波.脑胶质瘤miR-9、PPARγ表达水平及临床意义[J].中国临床神经外科杂志,2018,(01):17-19.[doi:10.13798/j.issn.1009-153X.2018.01.006]
　GAO Jian-feng,YAO Qing-he,LI Xiao-hui,et al.Expressions of miR-9 and PPARγ in human brain gliomas and their clinical meanings[J].,2018,(01):17-19.[doi:10.13798/j.issn.1009-153X.2018.01.006]
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脑胶质瘤miR-9、PPARγ表达水平及临床意义()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2018年01期
页码:
17-19
栏目:
论著
出版日期:
2018-01-25

文章信息/Info

Title:
Expressions of miR-9 and PPARγ in human brain gliomas and their clinical meanings
文章编号:
1009-153X（2018）01-0017-03
作者:
高剑峰　姚庆和　李晓辉　龙宇波　陈振波
作者单位：471009 河南洛阳，郑州大学附属洛阳中心医院神经外科（高剑峰、姚庆和、李晓辉、龙宇波、陈振波）
Author(s):
GAO Jian-feng;  YAO Qing-he;  LI Xiao-hui;  LONG Yu-bo;  CHEN Zhen-bo.
Department of Neurosurgery, Affiliated Central Hospital of Luoyang City, Zhengzhou University, Luoyang 471009, China
关键词:
脑胶质瘤; miR-9; 过氧化物体增殖剂活化受体γ; 表达
Keywords:
Gliomas;  MiR-9;  Peroxisome proliferator activated receptor γ;  Expression;  Prognosis
分类号:
R 739.41; Q 786
DOI:
10.13798/j.issn.1009-153X.2018.01.006
文献标志码:
A
摘要:
目的 探讨脑胶质瘤miR-9、过氧化物体增殖剂活化受体γ（PPARγ）表达水平及临床意义。方法 收集2013年2月至2015年2月脑胶质瘤手术标本76例，选取同期因颅脑损伤手术减压留取正常脑组织39例作为对照，采用实时荧光定量PCR检测miR-9、PPARγ mRNA表达水平，采用免疫组织化学方法检测PPARγ 蛋白表达水平。结果 脑胶质瘤组织miR-9 mRNA表达水平明显高于正常脑组织（P<0.05），而PPARγ mRNA及蛋白表达水平均明显低于正常脑组织（P<0.05）。高级别胶质瘤miR-9 mRNA表达阳性率（92.31%，36/39）明显高于低级别胶质瘤（62.16%，23/37；P<0.05），而PPARγ mRNA表达阳性率（48.72，19/39）明显低于低级别胶质瘤（75.68%，28/37；P<0.05）。脑胶质瘤组织miR-9与PPARγ表达呈明显负相关（r=-0.573, P<0.05）。miR-9高表达、PPARγ低表达是脑胶质瘤术后2年生存率的独立危险因素。结论 脑胶质瘤组织miR-9呈高表达，PPARγ呈低表达，二者呈明显负相关，且与脑胶质瘤病人生存率密切相关。
Abstract:
Objective To investigate the expressions of miR-9 and peroxisome proliferator activated receptor γ (PPARγ) in human brain gliomas and their clinical meanings. Methods The levels of miR-9 and PPARγ mRNA expressions were determined by qRT-PCR and the level of PPARγ protein expression was determined by immunohistochemical technique in 76 specimens of brain glioma tissues derived from the patients who were surgically treated in our hospital from February, 2013 to February, 2015, and 39 specimens of normal brain tissues derived from the patients with brain injury who were surgically treated in our hospital during the same period. The factors related to the prognoses were analyzed by COX regression model analysis. Results The level of miR-9 mRNA expression was significantly higher in the glioma tissues than that in the normal brain tissues (P<0.05). The level of PPARγ mRNA expression and the positive rate of PPARγ protein expression were significantly lower in the glioma tissues than those in the normal brain tissues (P<0.05). The positive rate of miR-9 mRNA expression was significantly higher and the positive rate of PPARγ mRNA expression was significantly lower in WHO grades Ⅲ~Ⅳ glioma tissues than those in WHO grades Ⅰ~Ⅱ glioma tissues (P<0.05). The independent risk factors related to the prognoses in the patients with gliomas included over expression of miR-9, low expression of PPARγ, high WHO grade glioma and incomplete resection of the tumors. The expression of miR-9 was significantly related negatively to that of PPARγ expression in the glioma tissues (r=-0.573, P<0.05). Conclusion WHO grade of the tumor and prognoses are closely to expressions of miR-9 and PPARγ in the tumorous tissues in the patients with gliomas.

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更新日期/Last Update: 2017-12-27