[1]黄昌恒　于耀宇　赵文可　何一川　姚自同.黄芪甲苷对大鼠蛛网膜下腔出血后迟发性脑血管痉挛的影响[J].中国临床神经外科杂志,2019,(02):98-101.[doi:10.13798/j.issn.1009-153X.2019.02.011]
　HUANG Chang-heng,YU Yao-yu,ZHAO Wen-ke,et al.Effect of astragaloside A on delayed cerebral vasospasm in rats after subarachnoid hemorrhage[J].,2019,(02):98-101.[doi:10.13798/j.issn.1009-153X.2019.02.011]
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黄芪甲苷对大鼠蛛网膜下腔出血后迟发性脑血管痉挛的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2019年02期
页码:
98-101
栏目:
论著
出版日期:
2019-02-20

文章信息/Info

Title:
Effect of astragaloside A on delayed cerebral vasospasm in rats after subarachnoid hemorrhage
文章编号:
1009-153X（2019）02-0098-04
作者:
黄昌恒　于耀宇　赵文可　何一川　姚自同
300162 天津，武警后勤学院附属医院神经外科（黄昌恒、于耀宇、赵文可、何一川、姚自同）
Author(s):
HUANG Chang-heng;  YU Yao-yu;  ZHAO Wen-ke;  HE Yi-chuan;  YAO Zi-tong
Department of Neurosurgery, Affiliated Hospital, Logistics College, Armed Police Command, Tianjin 300162, China
关键词:
蛛网膜下腔出血; 脑血管痉挛; 基底动脉; 黄芪甲苷; 大鼠
Keywords:
Subarachnoid hemorrhage;  Astragaloside A;  Basilar artery;  Rat
分类号:
R 743.9
DOI:
10.13798/j.issn.1009-153X.2019.02.011
文献标志码:
A
摘要:
目的 探讨黄芪甲苷对大鼠蛛网膜下腔出血（SAH）后迟发性脑血管痉挛的影响及机制。方法 将40只成年雄性SD大鼠随机分为假手术组、黄芪甲苷组（腹腔注射用0.1%二甲基亚砜助溶的黄芪甲苷溶悬液）、模型组和溶媒组（腹腔注射等体积0.1%二甲基亚砜），每组10只。采用颈内动脉刺破法制作SAH模型。SAH后24 h根据改良Garcia神经功能评分评定大鼠神经功能。SAH后6 d，HE染色测量基底动脉横截面积评估血管痉挛，免疫组化染色检测基底动脉p-Akt表达，免疫荧光染色检测基底动脉C-myc和α-SMA表达。结果 与假手术组相比，模型组和溶媒组改良Garcia神经功能评分明显降低（P<0.05），基底动脉横截面积明显减小（P<0.05），基底动脉α-SMA表达水平明显降低（P<0.05），基底动脉p-Akt和C-myc表达水平明显增加（P<0.05）；而模型组和溶媒组均无统计学差异（P>0.05）。与溶媒组相比，黄芪甲苷组改良Garcia神经功能评分明显提高（P<0.05），基底动脉横截面积明显增加（P<0.05），基底动脉α-SMA表达水平明显增加（P<0.05），基底动脉p-Akt和C-myc表达水平明显降低（P<0.05）。结论 黄芪甲苷可能通过抑制Akt信号通路下调C-myc的表达、促进α-SMA表达，影响SAH大鼠基底动脉血管平滑肌表型转换和增殖，增加基底动脉横截面积，从而缓解基底动脉痉挛。
Abstract:
Objective To investigate the effect of astragaloside A on the delayed cerebral vasospasm in rats after subarachnoid hemorrhage (SAH). Methods Forty adult male SD rats were randomly divided into sham-operation group, model group, vehicle group (intraperitoneal injection of equal volume 0.1% dimethyl sulfoxide) and astragalin A group (intraperitoneal injection of astragaloside A dissolved in 0.1% dimethyl sulfoxide), with 10 rats in each group. The SAH model was made by the method of internal carotid artery puncture. The neurological function of rats in each group was evaluated by the modified Garcia neurological function score 24 hours after SAH. Six days after SAH, the cross sectional area of basilar artery was measured by HE staining, the expression of p-Akt in basilar artery was detected by immunohistochemical staining, and the expressions of C-myc and alpha smooth muscle actin (α-SMA) in basilar artery were detected by immunofluorescence staining. Results Compared with the sham-operation group, the modified Garcia neurological function scores, the cross sectional areas of basilar artery and the expressions of α-SMA in basilar artery significantly decreased (P<0.05), and the expressions of p-Akt and C-myc in basilar artery significantly increased in model and vehicle groups (P<0.05). There was no statistical difference between model and vehicle groups (P>0.05). Compared with the vehicle group, the modified Garcia neurological function score, the cross sectional area of the basilar artery and the expression level of α-SMA in the basilar artery were significantly increased (P<0.05), and the expression levels of p-Akt and C-myc in basilar artery was significantly decreased in astragalin A group (P<0.05). Conclusion Astragaloside A can relieve the spasm of the basilar artery in rats after SAH by increasing the cross sectional area of the basilar artery which may involve in the inhibition of the Akt signaling pathway.

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备注/Memo

备注/Memo:
作者单位：300162 天津，武警后勤学院附属医院神经外科（黄昌恒、于耀宇、赵文可、何一川、姚自同）
通讯作者：于耀宇，E-mail：yuyaoyu666@aliyun.com
更新日期/Last Update: 2019-02-20