[1]袁国强 周旺宁 魏乃礼 王晓清 马克君 苏 愚 粱文涛 潘亚文.依脑膜瘤MLH1启动子甲基化水平及临床意义[J].中国临床神经外科杂志,2015,(03):158-160.[doi:10.13798/j.issn.1009-153X.2015.03.010]
 YUAN Guo-qiang,ZHOU Wang-ning,WEI Nai-li,et al.Level of MLH1 pomoter methylation and its clinical meanings in meningiomas[J].,2015,(03):158-160.[doi:10.13798/j.issn.1009-153X.2015.03.010]
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依脑膜瘤MLH1启动子甲基化水平及临床意义()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2015年03期
页码:
158-160
栏目:
论著
出版日期:
2015-03-30

文章信息/Info

Title:
Level of MLH1 pomoter methylation and its clinical meanings in meningiomas
文章编号:
1009-153X(2015)03-0158-03
作者:
袁国强 周旺宁 魏乃礼 王晓清 马克君 苏 愚 粱文涛 潘亚文
730030 兰州,兰州大学第二医院神经病学研究所(袁国强、王晓清、潘亚文),神经外科(周旺宁、魏乃礼、苏 愚、粱文涛),中心实验室(马克君)
通讯作者:潘亚文,E-mail:panyawen666@sohu.com
Author(s):
YUAN Guo-qiang1 ZHOU Wang-ning2 WEI Nai-li2 WANG Xiao-qing1 MA Ke-jun3 Pan Ya-wen1.
1. Neurology Institute, The Second Hospital, Lanzhou University, Lanzhou 730030, China; 2. Department of Neurosurgery, The Second Hospital, Lanzhou University, Lanzhou 730030, China; 3. Center Laboratory, The Second Hospital, Lanzhou University, Lanzhou 730030, China
关键词:
脑膜瘤侵袭性mutL同源基因1启动子甲基化
Keywords:
Meningioma Invasiveness MLH1 promoter Methylation Level
分类号:
R 739.41; Q 786
DOI:
10.13798/j.issn.1009-153X.2015.03.010
文献标志码:
A
摘要:
目的 探讨脑膜瘤mutL同源基因1(MLH1)启动子甲基化水平与脑膜瘤侵袭性的相关性。方法 从我院标本库随机选取64例脑膜瘤石蜡包埋组织标本,其中侵袭性脑膜瘤26例,非侵袭性脑膜瘤38例。应用逆转录-PCR检测MLH1 mRNA表达水平,应用特异高分辨率熔解(MS-HRM)曲线检测不同侵袭性脑膜瘤MLH1启动子甲基化水平。结果 侵袭性脑膜瘤MLH1 mRNA含量比非侵袭性脑膜瘤显著降低(P<0.05)。MS-HRM曲线分析显示,侵袭性脑膜瘤MLH1启动子区高度甲基化水平为0~1%所占比例(7.7%,2/26)显著低于非侵袭性脑膜瘤(55.3%,21/38;P<0.05)。结论 MLH1启动子区甲基化可能下调MLH1的表达,进而调控脑膜瘤侵袭性行为。
Abstract:
Objective To establish a method to determine mut L momolog1 (MLH1) promoter methylation level in the meningiomas and analyze the relationship between MLH1 methylation level and meningioma invasiveness. Methods DNA was extracted from 64 samples of the meningiomas, of which, 26 were invasive meningiomas and 38 non-invasive ones, and the methylation modification of DNA was performed according to instruction book. The standard methylation MLH1 DNA was diluted by non-methylation MLH1 DNA in different proportion of 100%, 50%, 25%, 12.5% and 1% to establish the standard methylation sensitive high resolution melting curve. MLH1 promoter methylation level was determined quantitatively by the method of MS-HRM method in all the invasive meningiomas and non invasive meningiomas. The relationship of MLH1 promoter methylation level with meningioma invasiveness was analyzed. Result MLH1 promoter methylation level was significantly higher in the invasive meningiomas than that in the non invasive meningiomas (P<0.01). Conclusion The present result suggests that MLH1 promoter methylation level may play an important role in the pathogenesis of the invasiveness of the meningiomas.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:兰州大学中央高校基本科研业务费专项资金(lzujbky-2013-146)
更新日期/Last Update: 2015-03-30