[1]彭泽生 田道锋 张申起 陈谦学.miR-370-3p对胶质母细胞瘤U87-MG细胞株增殖能力的影响[J].中国临床神经外科杂志,2016,(04):223-226.[doi:10.13798/j.issn.1009-153X.2016.04.010]
 PENG Ze-sheng,TIAN Dao-feng,ZHANG Shen-qi,et al.Effects of MircoRNA-370-3p on proliferation of glioma cell line U87-MG and its potential mechanism[J].,2016,(04):223-226.[doi:10.13798/j.issn.1009-153X.2016.04.010]
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miR-370-3p对胶质母细胞瘤U87-MG细胞株增殖能力的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2016年04期
页码:
223-226
栏目:
论著
出版日期:
2016-04-25

文章信息/Info

Title:
Effects of MircoRNA-370-3p on proliferation of glioma cell line U87-MG and its potential mechanism
文章编号:
1009-153X(2016)04-0223-04
作者:
彭泽生 田道锋 张申起 陈谦学
430060 武汉,武汉大学人民医院神经外科(彭泽生、田道锋、张申起、陈谦学)
通讯作者:田道锋,E-mail:tiandaofeng@hotmail.com
Author(s):
PENG Ze-sheng TIAN Dao-feng ZHANG Shen-qi CHEN Qian-xue.
Department of Neurosurgery, Renmin Hospital, Wuhan University, Wuhan 430060, China
关键词:
胶质瘤U87-MG细胞微小RNAmiR-370-3p叉头框蛋白M1细胞增殖
Keywords:
Glioma U 87-MG cells MircoRNA-370-3p Proliferation FoxM1
分类号:
R 739.41; R 73-34
DOI:
10.13798/j.issn.1009-153X.2016.04.010
文献标志码:
A
摘要:
目的 探讨微小RNA-370-3p(miR-370-3p)对人脑胶质瘤细胞系U87-MG增殖能力的影响及其机制。方法 将常规培养的U87-MG细胞分为空白组、无义序列转染组和模拟物转染组,后两组分别转染miRNA无义序列及miR-370-3p模拟物,qRT-PCR法检测其转染效率,免疫印迹法检测转染细胞叉头框蛋白M1(FoxM1)的表达;采用EdU法评估细胞的增殖能力,采用平板克隆形成实验检测细胞的增殖能力。结果 与空白组和无义序列转染组比较,模拟物转染组细胞miR-370-3p表达显著增加(P<0.05),而FoxM1的蛋白表达显著减少(P<0.05);而且模拟物转染组U87-MG细胞增殖能力及克隆形成率均明显减少(P<0.05)。结论 miR-370-3p能够抑制U87-MG细胞的增殖能力,可能与减少FoxM1的表达有关。
Abstract:
Objective To explore the effect of MircoRNA (miRNA) -370-3p on the proliferation of U87-MG glioblastoma cells and its mechanism. Methods The cultured U87-MG cells were divided into blank group, mimics transfection group (transfected with Has-miRNA-370-3p mimics) and negative control group (transfected with miRNA-ctrl). Transfections of Has-miRNA-37-3p mimics were mediated by lipofectamine 2000 U87-MG cells. The transfection efficiency were detected by quantative real-time PCR. The expression of Forkhead M1 (FoxM1) protein were investigated by Western blotting 48 h after the transfection. The cell proliferation ability was evaluated by 5-ethynyl-2’-deoxyuridine (EdU) incorporation and colony formation assaies 72 h after the transfection. Results The expression of miRNA-370-3p increased and FoxM1 protein expression decreased significantly in U87-MG cells in the mimics transfection group compared with those in the blank group and negative control group. EdU assay showed that the short-term ability of the U87-MG cells proliferation was significantly lower in the mimics transfected group than those in the other two groups (P<0.05). The colony formation assay showed that the long-term ability of the U87-MG cells proliferation was significantly lower in the mimics transfection group than those in the other two groups (P<0.05). Conclusion It is suggested that upregulation of miRNA-370-3p expression can inhibit proliferation of U87-MG cells, probably by downregulating expression of FoxM1.

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备注/Memo

备注/Memo:
基金项目:湖北省自然科学基金(2011CDB493);湖北省卫生厅科研项目(JX6B15)
更新日期/Last Update: 2016-04-30