[1]冯　驰　郭双毅　程龙海　罗　杰.氯喹通过抑制自噬促进TRAIL诱导的胶质瘤细胞凋亡[J].中国临床神经外科杂志,2016,(04):227-229.[doi:10.13798/j.issn.1009-153X.2016.04.011]
　FENG Chi,GUO Shuang-yi,CHENG Long-hai,et al.Chloroquine promotes TRAIL-induced apoptosis of glioma cells through inhibition of autophagy[J].,2016,(04):227-229.[doi:10.13798/j.issn.1009-153X.2016.04.011]
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氯喹通过抑制自噬促进TRAIL诱导的胶质瘤细胞凋亡()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2016年04期
页码:
227-229
栏目:
论著
出版日期:
2016-04-25

文章信息/Info

Title:
Chloroquine promotes TRAIL-induced apoptosis of glioma cells through inhibition of autophagy
文章编号:
1009-153X（2016）04-0227-03
作者:
冯　驰　郭双毅　程龙海　罗　杰
420073 武汉，武汉大学中南医院神经外科（冯　驰）；442000 湖北，十堰市太和医院神经外科（郭双毅、程龙海、罗　杰）
Author(s):
FENG Chi1;  GUO Shuang-yi2;  CHENG Long-hai2;  LUO Jie1; 2.
1. Department of Neurosurgery, Zhongnan Hospital, Wuhan University, Wuhan 430072, China; 2. Department of Neurosurgery, Shiyan Taihe Hospital, Shiyan 442000, China
关键词:
胶质瘤; U251细胞; 肿瘤坏死因子相关凋亡诱导配体; 氯喹; 细胞凋亡; 细胞自噬
Keywords:
Tumor necrosis factor-related apoptosis-inducing ligand;  Chloroquine;  U251 cell;  Apoptosis;  Autophagy
分类号:
R 739.41; R 730.59
DOI:
10.13798/j.issn.1009-153X.2016.04.011
文献标志码:
A
摘要:
目的 探讨氯喹对肿瘤坏死因子相关凋亡诱导配体（TRAIL）诱导的胶质瘤细胞凋亡的影响。方法 体外培养人脑胶质瘤细胞U251和Hela细胞，分为对照组、氯喹组、重组人TRAIL蛋白（rhTRAIL）组和联用组（氯喹与rhTRAIL联合应用）；MTT法检测细胞活力，Annexin V-FITC/PI凋亡检测试剂盒检测细胞凋亡，共聚焦显微镜检测GFP-LC3的分布判断细胞自噬水平，Western Blot检测cleaved caspase-8的表达水平。结果 氯喹和rhTRAIL均显著增加U251细胞和Hela细胞自噬水平，而且联用较单独应用自噬水平更高，Hela细胞自噬水平明显高于U251细胞。U251细胞和Hela细胞增殖抑制率和细胞凋亡率：氯喹组与对照组均无统计学差异（P>0.05），rhTRAIL和联用组均明显高于氯喹组（P<0.05），联用组明显高于rhTRAIL组（P<0.05）。氯喹组和对照组Cleaved caspases-8水平无统计学差异（P>0.05），rhTRAIL和联用组均明显增高（P<0.05），联用组明显高于rhTRAIL组（P<0.05）。结论 氯喹能通过抑制细胞自噬，增强TRAIL诱导的U251细胞凋亡。
Abstract:
Objective To study the mechanism of glioma cells apoptosis induced by Chloroquine (CQ). Methods Human glioma U251 cells were treated with recombined human tumor necrosis factor-related apoptosis-inducing ligand (rhTRAIL) and/or CQ. The cells vitality was detected by MTT assay and the U251 cells apoptosis was detected by Annexin V-FITC/PI Apoptosis Detection Kit. The GFP-LC3 distribution was detected by confocal microscope. The expression level of caspases-8 was detected by Western Blot. Results The autophagy level of the U251 cells treated with rhTRAIL increased when rhTRAIL induced the U251 cells apoptosis. The growth inhibition rate [(47.11±0.18)%] and the cell apoptosis rate [(34.31±0.97)] of U251 cells treated with rhTRAIL and CQ were significantly higher respectively than those [(23.88±0.48)% and (19.68±0.67)% respectively] of U251 cells treated with rhTRAIL only (P<0.05). The level of cleared caspases-8 expression was significantly higher in the U251 cells treated by rhTRAIL and CQ than those in U251 cells treated by rhTRAIL only (P<0.05). Conclusion It is suggested that CQ may enhance rhTRAIL-induced apoptosis through inhibiting the autophagy induced by rhTRAIL.

参考文献/References:

[1] Stuckey DW, Shah K. TRAIL on trial: preclinical advances in cancer therapy [J]. Trends Mol Med, 2013, 19(11): 685- 694.
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备注/Memo

备注/Memo:
基金项目：湖北省自然科学基金（2011CDB493）；湖北省卫生厅科研项目（JX6B15）
更新日期/Last Update: 2016-04-30