[1]常英男 王 策 赵天书.SOX2基因在胶质瘤中的表达及生物学作用[J].中国临床神经外科杂志,2016,(05):270-273.[doi:10.13798/j.issn.1009-153X.2016.05.005]
 CHANG Ying-nan,WANG Ce,ZHAO Tian-shu..Expression of SOX2 gene and its biological role in human gliomas[J].,2016,(05):270-273.[doi:10.13798/j.issn.1009-153X.2016.05.005]
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SOX2基因在胶质瘤中的表达及生物学作用()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2016年05期
页码:
270-273
栏目:
论著
出版日期:
2016-05-25

文章信息/Info

Title:
Expression of SOX2 gene and its biological role in human gliomas
文章编号:
1009-153X(2016)05-0270-04
作者:
常英男 王 策 赵天书
作者单位:150006 哈尔滨,哈尔滨医科大学附属第四医院神经外科(常英男、王 策、赵天书)
Author(s):
CHANG Ying-nan WANG Ce ZHAO Tian-shu.
Department of Neurosurgery, The Fourth Affiliated Hospital, Harbin Medical University, Harbin 150006, China
关键词:
胶质瘤SOX2基因甲基化肿瘤学特性
Keywords:
Glioma SOX2 gene expression Methylation Tumor characteristics
分类号:
R 739.41; Q 786
DOI:
10.13798/j.issn.1009-153X.2016.05.005
文献标志码:
A
摘要:
目的 探讨SOX2基因在人胶质瘤中的表达水平、甲基化状态以及生物学作用。方法 选取人胶质瘤组织标本108例,正常脑组织30例。采用PCR的方法检测SOX2基因的表达水平和甲基化状态。MTT和软琼脂集落形成试验检测SOX2对胶质瘤U87和U251细胞的生物学作用。结果 SOX2在正常脑组织中的相对表达水平明显低于胶质瘤组织(PP<0.01)。过量表达sox2基因可促进胶质瘤u87和u251细胞增殖和集落形成能力。>结论 SOX2基因在胶质瘤组织高表达,其在胶质瘤中的表达水平可能受启动子区域甲基化状态影响,SOX2基因影响胶质瘤的肿瘤学特性。
Abstract:
Objective To explore the expression level, methylation status and biological role of SOX2 in human gliomas. Methods The expression levels and the methylation status of SOX2 were determined by PCR technique in 108 specimens of glioma tissues and 30 specimens of normal brain tissues. The roles of SOX2 in the tumor characteristics of glioma cell lines were evaluated by MTT and colony-formation assay. Results The expression level (0.49±0.2) of SOX2 was significantly higher in the glioma tissues than that (0.154±0.076) in the normal brain tissues (PP<0.01), which="" was="" significantly="" lower="" than="" that="" (100.0%)="" in="" the="" normal="" brain="" tissues="" (P<0.05). mtt="" and="" colony-formation="" assay="" showed="" that="" sox2="" could="" promote="" glioma="" cells="" proliferation="" anchorage="" independent="" growth="" capacities="" (P<0.05).>Conclusions Upregulation of SOX2 expression was found in the human gliomas tissues. The expression of SOX2 was influenced by its promoter methylation status. Overexpression of SOX2 in glioma cell lines can promote glioma cells proliferation.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:黑龙江省科技厅课题(D201140)
通讯作者:赵天书,E-mail:1097976852@qq.com
更新日期/Last Update: 2016-05-30