[1]沈旭辉　邹建军　李付勇.溶血磷脂酸对大鼠颈动脉损伤后血管炎性反应的影响[J].中国临床神经外科杂志,2017,(01):31-33,57.[doi:10.13798/j.issn.1009-153X.2017.01.011]
　SHEN Xu-hui,ZOU Jian-jun,LI Fu-yong..Effect of lysophosphatidic acid on vascular inflammatory reaction after carotid injury in rats[J].,2017,(01):31-33,57.[doi:10.13798/j.issn.1009-153X.2017.01.011]
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溶血磷脂酸对大鼠颈动脉损伤后血管炎性反应的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2017年01期
页码:
31-33,57
栏目:
论著
出版日期:
2017-01-18

文章信息/Info

Title:
Effect of lysophosphatidic acid on vascular inflammatory reaction after carotid injury in rats
文章编号:
1009-153X（2017）01-0031-03
作者:
沈旭辉　邹建军　李付勇
110016 沈阳，辽宁省人民医院神经外科
Author(s):
SHEN Xu-hui;  ZOU Jian-jun;  LI Fu-yong.
The Third Department of Neurosurgery, The People's Hospital of Liaoning Province,Shenyang110016, China
关键词:
颈动脉; 内膜损伤; 溶血磷脂酸; Toll样受体4; 大鼠
Keywords:
Objective To research effect of lysophosphatidic acid (LPSA) on vascular inflammatory reaction in rats with carotid injury. Methods Twenty-four SD rats were randomly divided into three groups of 8 animals each;  i.e. sham operation group;  model group;  and
分类号:
R 743
DOI:
10.13798/j.issn.1009-153X.2017.01.011
文献标志码:
A
摘要:
目的 探讨溶血磷脂酸（LPA）对大鼠颈动脉内膜损伤后血管炎性反应的影响。方法 将24只SD大鼠随机分为假手术组、模型组和LPA组，每组8只。采用球囊导管法建立大鼠颈动脉内膜损伤模型。LPA组造模后经导管注射LPA（100 μl）干预，模型组注射磷酸盐缓冲液。造模后14 d处死大鼠，取出损伤部位颈总动脉，行HE染色观察内膜的增生情况，Toll样受体4（TLR4）免疫组化染色和免疫印迹法检测TLR4蛋白表达，采用PCR方法检测TLR4 mRNA表达。结果 HE染色分析，假手术组颈动脉平滑肌细胞排列整齐，中膜内膜无增厚，无明显狭窄；模型组可见颈动脉轻度损伤，部分区域细胞排列紊乱，内膜和中膜亦可见增厚，血管轻度狭窄；LPA组可见颈动脉损伤区域平滑肌细胞排列紊乱，内膜和中膜有明显的增厚，内膜中膜界限不清，血管狭窄较重。模型组TLR4蛋白和mRNA表达水平明显高于假手术组（P<0.05），而LPA组均明显高于模型组（P<0.05）。结论 LPA促进大鼠颈动脉内膜损伤后炎性反应，其机制可能与促进TLR4表达密切相关。
Abstract:
Objective To research effect of lysophosphatidic acid (LPSA) on vascular inflammatory reaction in rats with carotid injury. Methods Twenty-four SD rats were randomly divided into three groups of 8 animals each, i.e. sham operation group, model group, and LPSA treatment group. The unilateral carotid artery injury model was built in the model and LPSA treatment groups, in which 100 μl phosphate buffer and LPSA were injected into the injured carotid arteries respectively. The left external carotid artery and its branches were ligated only in the sham operation group, in which the rats did not receive drug treatment. The rats were sacrificed in order to take the injured carotid arteries in all the groups 14 days after the establishment of the model. The histological changes in the injured carotid arteries were observed by microscope after HE staining. The expression level of toll like receptor 4 (TLR4) protein and mRNA in the carotid arteries were determined by immunohistochemical test, Western blot test and RT-PCR test respectively in all the groups. Results The inflammatory reaction was more significant in LPSA group than that in the model group, in which the inflammatory reaction was more significant than that in the sham operation group. The expression levels of TLR4 protein and mRNA were significantly higher in LPSA group than those in the model group (P<0.05), in which the expression levels of TLR4 protein and mRNA were significantly higher than those in the sham operation group (P<0.05). Conclusion That LPSA can promote inflammatory reaction after the carotid injury may be produced by the upregulation of expression level of TLR4 protein and mRNA in the injured carotid arteries caused by LPSA in the rats.

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更新日期/Last Update: 2016-10-28