[1]穆寅东　赵天书　谢　清.脑胶质瘤SOX7基因的甲基化状态及临床意义[J].中国临床神经外科杂志,2019,(02):90-92.[doi:10.13798/j.issn.1009-153X.2019.02.009]
　MU Yin-dong,ZHAO Tian-shu,XIE Qing..Methylation state of SOX9 in human glioma and its clinical meanings[J].,2019,(02):90-92.[doi:10.13798/j.issn.1009-153X.2019.02.009]
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脑胶质瘤SOX7基因的甲基化状态及临床意义()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2019年02期
页码:
90-92
栏目:
论著
出版日期:
2019-02-20

文章信息/Info

Title:
Methylation state of SOX9 in human glioma and its clinical meanings
文章编号:
1009-153X（2019）02-0090-03
作者:
穆寅东　赵天书　谢　清
157011 黑龙江牡丹江，牡丹江医学院组胚教研室（穆寅东）；150006 哈尔滨，哈尔滨医科大学附属第四医院神经外科（赵天书）；100038 北京，首都医科大学附属北京世纪坛医院医学检验科/尿液细胞分子诊断北京市重点实验室（谢　清）
Author(s):
MU Yin-dong1;  ZHAO Tian-shu2;  XIE Qing3.
1. Department of Histology and Embryology, Mudanjiang Medical College, Mudanjiang 157011, China; 2. Department of Neurosurgery, The Fourth Affiliated Hospital, Harbin Medical University, Harbin 150006, China; 3. Department of Clinical Laboratory, Beijing
关键词:
胶质瘤; SOX7基因; 甲基化; 预后
Keywords:
Human glioma;  SOX7 gene;  Methylation;  Survival time
分类号:
R 739.41; Q 786
DOI:
10.13798/j.issn.1009-153X.2019.02.009
文献标志码:
A
摘要:
目的 探讨脑胶质瘤SOX7基因的甲基化状态及其与病人预后的关系。方法 收集2013年6月至2015年1月手术切除的胶质瘤标本131例，另取颅脑损伤内减压术中切除的正常脑组织标本32例作为对照。应用甲基化特异PCR及RT-PCR方法检测SOX7基因的甲基化状态及mRNA表达水平。采用Kplan-Meier法分析生存曲线。结果 胶质瘤SOX7基因甲基化率（71.8%，94/131）明显高于正常脑组织（32.4%，11/34；P<0.05）。高级别胶质瘤SOX7甲基化率（81.01%，64/79）明显高于低级别胶质瘤（57.69%，30/52）。胶质瘤SOX7 mRNA水平明显低于正常脑组织（P<0.05）。SOX7基因甲基化组生存期较非甲基化组明显缩短（P<0.01）。结论 胶质瘤SOX7基因甲基化率升高，SOX7在胶质瘤中表达水平下调，SOX7基因的甲基化状态与病人生存期密切相关。
Abstract:
Objective To detect the methylation status and expression level of SOX7 in the human glioma and normal brain tissues, and evaluate their relationship with the tumorigenesis and prognosis in the patients with gliomas. Methods The methylation states and expression levels of SOX7 were determined respectively by methylation specific PCR and RT-PCR in 131 samples of gliomas tissues and 34 samples of the normal brain tissues. The analysis of survival curve was performed by Kplan-Meier method. Results The methylation rate of SOX7 (71.8%, 94/131) was significantly higher in the glioma tissues than that 32.4% (11/34) in the normal brain tissues (P<0.01). The methylation rate of SOX7 (81.01%, 64/79) was significantly higher in the WHO grade Ⅲ~Ⅳ gliomas tissues than that (57.69%, 30/52) in the WHO grade Ⅰ~Ⅱ gliomas tissues. The expression level of SOX7 mRNA was (0.43±0.15) significantly higher in the normal brain tissues than that (0.34±0.17) in the glioma tissues (P<0.01). The level of SOX7 mRNA expression (0.38±0.18) was significantly higher in the WHO grade Ⅰ~Ⅱ gliomas tissues than that (0.31±0.66) in the WHO grade Ⅲ~Ⅳ gliomas tissues (P<0.01). The survival time was significantly shorter in the patients with methylation of SOX7 gliomas than that in the patients with non-methylation of SOX7 gliomas (P<0.05). Conclusions SOX7 is hypermethylated and its expression level was downregulated in human glioma tissues. The methylation status of SOX7 in the gliomas tissues was closely correlated with glioma patient’s survival time.

参考文献/References:


[1] Liu H, Chen X, Xue W, et al. Recombinant epidermal growth factor-like domain-1 from coagulation factor Ⅶfunctiona- lized iron oxide nanoparticles for targeted glioma magnetic resonance imaging [J]. Int J Nanomed, 2016, 11: 5099- 5108.
[2] Chew LJ, Gallo V. The Yin and Yang of Sox proteins: Acti- vation and repression in development and disease [J]. J Neurosci Res, 2009, 87(15): 3277-3287.
[3] Gandillet A, Serrano AG, Pearson S, et al. Sox7-sustained expression alters the balance between proliferation and differentiation of hematopoietic progenitors at the onset of blood specification [J]. Blood, 2009, 114(23): 4813-4822.
[4] Nelson TJ, Chiriac A, Faustino RS, et al. Lineage specifica- tion of Flk-1+ progenitors is associated with divergent Sox7 expression in cardiopoiesis [J]. Differentiation, 2009, 77(3): 248-255.
[5] Cermenati S, Moleri S, Cimbro S, et al. Sox18 and Sox7 play redundant roles in vascular development [J]. Blood, 2008, 111(5): 2657-2666.
[6] Francois M, Koopman P, Beltrame M. SoxF genes: key players in the development of the cardio-vascular system [J]. Int J Biochem Cell Biol, 2010, 42(3): 445-448.
[7] Zheng Z, Liu J, Yang Z, et al. MicroRNA-452 promotes stem-like cells of hepatocellular carcinoma by inhibiting Sox7 involving Wnt/beta-catenin signaling pathway [J]. Oncotarget, 2016, 7(19): 28000-28012.
[8] Louis DN, Ohgaki H, Wiestler OD, et al. The 2007 WHO classification of tumours of the central nervous system [J]. Acta Neuropathol, 2007, 114(2): 97-109.
[9] 郭艳丽，邓良勇，郭炜，等. 转录因子SOX7基因在贲门腺 癌中的异常表达及其甲基化状态[J]. 中国肿瘤生物治疗 杂志，2014，（6）：652-657.
[10] Hayano T, Garg M, Yin D, et al. SOX7 is down-regulated in lung cancer [J]. J Exp Clin Cancer Res, 2013, 32(17): 1- 11.
[11] Takash W, Canizares J, Bonneaud N, et al. SOX7 transcrip- tion factor: sequence, chromosomal localisation, expression, transactivation and interference with Wnt signalling [J]. Nucleic Acids Res, 2001, 29(21): 4274-4283.
[12] Liu H, Mastriani E, Yan ZQ, et al. SOX7 co-regulates Wnt/ beta-catenin signaling with Axin-2: both expressed at low levels in breast cancer [J]. Sci Rep, 2016, 6: 1-14.

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备注/Memo

备注/Memo:
基金项目：北京市自然科学基金（7174315）
作者单位：157011 黑龙江牡丹江，牡丹江医学院组胚教研室（穆寅东）；150006 哈尔滨，哈尔滨医科大学附属第四医院神经外科（赵天书）；100038 北京，首都医科大学附属北京世纪坛医院医学检验科/尿液细胞分子诊断北京市重点实验室（谢　清）
通讯作者：谢　清，E-mail：xieqing@bjmu.edu.cn
更新日期/Last Update: 2019-02-20