[1]丁华强 廖 帅 汪棋笙 全星运 刘胜杰 廖落星 刘 亮.亚低温通过抑制AIF核转位改善大鼠颅脑损伤[J].中国临床神经外科杂志,2021,26(09):702-705.[doi:10.13798/j.issn.1009-153X.2021.09.015]
 DING Hua-qiang,LIAO Shuai,WANG Qi-sheng,et al.Effects of mild hypothermia on nucleus translocation of apoptosis inducing factor in injured cerebral tissues of adult rats after traumatic brain injury[J].,2021,26(09):702-705.[doi:10.13798/j.issn.1009-153X.2021.09.015]
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亚低温通过抑制AIF核转位改善大鼠颅脑损伤()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
26
期数:
2021年09期
页码:
702-705
栏目:
实验研究
出版日期:
2021-09-25

文章信息/Info

Title:
Effects of mild hypothermia on nucleus translocation of apoptosis inducing factor in injured cerebral tissues of adult rats after traumatic brain injury
文章编号:
1009-153X(2021)09-0702-04
作者:
丁华强 廖 帅 汪棋笙 全星运 刘胜杰 廖落星 刘 亮
401120,重庆市渝北区人民医院神经外科(丁华强);621000 四川,绵阳市第三人民医院神经外科(廖 帅);646200 四川,合江县人民医院神经外科(汪棋笙);646000 四川泸州,西南医科大学附属医院神经外科(全星运、刘胜杰、刘 亮);400000,重庆市南岸区人民医院神经外科(廖落星)
Author(s):
DING Hua-qiang1 LIAO Shuai2 WANG Qi-sheng3 QUAN Xing-yun4 LIU Sheng-jie4 LIAO Luo-xing5 LIU Liang4.
1. Department of Neurosurgery, Chongqing Yubei District People’s Hospital, Chongqing 401120, China; 2. Department of Neurosurgery, Mianyang No. 3 People’s Hospital, Mianyang 621000, China; 3. Department of Neurosurgery, People’s Hospital of Hejiang City,
关键词:
颅脑损伤亚低温细胞凋亡凋亡诱导因子神经保护作用
Keywords:
Traumatic brain injury Mild hypothermia Cell apoptosis Apoptosis inducing factor Neuroprotection
分类号:
R 651.1+5; R 454
DOI:
10.13798/j.issn.1009-153X.2021.09.015
文献标志码:
A
摘要:
目的 探讨亚低温对颅脑损伤(TBI)大鼠脑组织凋亡诱导因子(AIF)核转位的影响。方法 将36只成年雄性SD大鼠随机分为假手术组、TBI组、亚低温组,每组12只。采用控制性皮质撞击建立TBI模型,亚低温组大鼠给予亚低温处理。TBI后24 h,HE染色观察大鼠脑组织病理学变化;免疫组织化学方法检测大鼠脑组AIF的表达部位;免疫印迹法检测损伤脑组织线粒体和细胞核AIF、caspase-3的表达情况。结果 HE染色结果显示,TBI后,损伤侧脑组织可见沿灰白质界面的挫伤和出血,亚低温组挫伤和出血灶明显减轻。免疫印迹法检测结果显示,TBI后,损伤脑组织caspase-3表达量明显增加(P<0.01),细胞核AIF表达量明显增加(P<0.01),而线粒体AIF表达量明显降低(P<0.05);亚低温组损伤脑组织caspase-3表达量明显下降(P<0.01),细胞核AIF表达量明显下降(P<0.01),而线粒体AIF表达量明显升高(P<0.05)。免疫组织化学染色结果显示,假手术组AIF位于大脑皮质和海马神经元细胞核外;TBI组后,损伤侧皮质及海马区AIF从线粒体转移至细胞核内的阳性细胞数量明显增多(P<0.01);亚低温组损伤侧皮质及海马区AIF发生核内转移的阳性细胞数量减少(P<0.01)。结论 亚低温可能通过抑制AIF的核转位减轻颅脑损伤后神经细胞凋亡,从而起到神经保护作用。
Abstract:
Objective To explore the effect of mild hypothermia (MHT) on the nucleus translocation of apoptosis inducing factor (AIF) in injured cerebral tissues of adult rats after traumatic brain injury (TBI). Methods Thirty six adult male SD rats were randomly divided into sham group, TBI group and MHT group, with 12 rats in each. TBI was induced by controlled cortical impact. The rats in the MHT group were treated with MHT. Twenty-four hours after TBI, the pathological changes of injured cerebral tissues were analyzed with HE staining; AIF translocation was dtected by immunohistochemical staining; expressions of AIF and caspase-3 were analyzed by western blotting. Results HE staining showed that contusion and hemorrhage along the gray white matter interface were visible in TBI group, and they were significantly improved in MHT group. Western blotting showed that the caspase-3 expression in the injured cerebral tissues was significantly increased (P<0.01), the AIF expression in the nucleus was significantly increased (P<0.01), and the AIF expression in mitochondria was significantly reduced (P<0.05) in TBI group; the expressions of caspase-3 and AIF in MHT group were significantly reversed compared to TBI group (P<0.05). Immunohistochemical staining showed that the number of positive cells transferred from mitochondria to nucleus of AIF in the injured cortex and hippocampus increased significantly in TBI group (P<0.01), and it was significantly decreased in MHT group (P<0.01). Conclusions MHT has a neuroprotective effect on TBI, which may be by inhibiting the nuclear translocation of AIF so as to reduce the apoptosis of nerve cells.

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备注/Memo

备注/Memo:
通讯作者:刘 亮,E-mail:liulst@163.com
更新日期/Last Update: 1900-01-01