[1]唐菁华　孙秀勤　王建军.阿托伐他汀对颅脑损伤大鼠神经元凋亡的抑制作用[J].中国临床神经外科杂志,2020,(04):227-230.[doi:10.13798/j.issn.1009-153X.2020.04.012]
　TANG Jing-hua,SUN Xiu-qin,WANG Jian-jun..Inhibitive effect of atorvastatin on neuron apoptosis in rats with traumatic brain injury and its mechanism[J].,2020,(04):227-230.[doi:10.13798/j.issn.1009-153X.2020.04.012]
点击复制

阿托伐他汀对颅脑损伤大鼠神经元凋亡的抑制作用()
分享到：

《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2020年04期
页码:
227-230
栏目:
实验研究
出版日期:
2020-04-30

文章信息/Info

Title:
Inhibitive effect of atorvastatin on neuron apoptosis in rats with traumatic brain injury and its mechanism
文章编号:
1009-153X（2020）04-0227-04
作者:
唐菁华　孙秀勤　王建军
637000 四川南充，川北医学院附属医院儿科（唐菁华、孙秀勤、王建军）
Author(s):
TANG Jing-hua;  SUN Xiu-qin;  WANG Jian-jun.
Department of Pediatrics, Affiliated Hospital, North Sichuan Medical College, Nanchong 637000, China
关键词:
颅脑损伤; 阿托伐他汀; 大鼠; 神经元; 细胞凋亡
Keywords:
Traumatic brain injury;  Atorvastatin;  Rats;  Apoptosis;  Mechanism
分类号:
R 651.1+5; Q 786
DOI:
10.13798/j.issn.1009-153X.2020.04.012
文献标志码:
A
摘要:
目的 探讨阿托伐他汀对颅脑损伤（TBI）大鼠神经元凋亡的抑制作用及机制。方法 30只SD大鼠随机分为假手术组、模型组和阿托伐汀他组，各10只。采用液压打击法制作TBI模型，阿托伐他汀组连续灌胃阿托伐他汀2周（1 mg/kg/d），假手术组、模型组灌胃等体积生理盐水。给药结束后第2天，参照Zea Longa 5分制标准进行神经功能评分。酶联免疫吸附法检测血清肿瘤坏死因子（TNF-α）、白介素（IL）-6和IL-1β水平；免疫印迹法检测损伤脑组织Toll样受体4（TLR4）、核转录因子（NF）-κB p65、p-IκB、cleaved Caspase-3蛋白表达；末端标记法检测神经元凋亡；HE染色观察脑组织病理变化。结果 与模型组相比，阿托伐他汀组大鼠神经功能缺损评分、细胞凋亡率、血清IL-6、TNF-α和IL-1β水平明显改善（P<0.05），损伤脑组织TLR4、NF-KB p65、p-IκB、cleaved Caspase-3水平明显下调（P<0.05）；HE染色显示脑组织损伤程度明显减轻。结论 阿托伐他汀可能通过抑制TLR4/NF-кB信号通路，抑制TBI大鼠的神经元细胞凋亡，促进神经功能恢复。
Abstract:
Objective To analyze inhibitive effect of atorvastatin on neuron apoptosis in the rats with traumatic brain injury (TBI) and its mechanism. Methods Thirty SD rats were randomly divided into 3 groups of 10 animals each, i.e. sham operation group, model group and atorvastatin group. TBI models were made by fluid-percussion technique. The oral administration of atorvastatin (1 mg/kg/d) was performed for 2 weeks in the rats of atorvastatin group after the successful establishment of TBI model. The neurological function defects were determined by Zea Longa method after the treatment. The serum levels of tumor necrosis factor (TNF-α), interleukin (IL)-6 and IL-1β were detected by enzyme linked immunosorbent assay. The expressions of Toll-like receptor 4 gene (TLR4), nuclear factor (NF)-κB p65, p-IκB and cleaved Caspase-3 protein in the injured brain tissues were determined. The apoptosis of the neurons in the injured brain tissues was determined by TdT-mediated dUTP nick end labeling and the pathological change in the injured brain tissues was observed by HE staining. Results The scores of neurological function defects, apoptosis rate of the neurons, the levels of serum IL-6, TNF-α and IL-1β and the levels of TLR4, NF-κB p65, p-IκB and cleaved Caspase-3 protein expressions in the injured brain tissues were significantly lower in atorvastatin group than those in the model group (P<0.05). HE staining showed that the degree of damage to the brain tissues was obviously reduced in atorvastatin group compared to that in the model group. Conclusion Atorvastatin may inhibit apoptosis of neurons by inhibiting TLR4/NF-кB signaling pathway in the rats with TBI.

参考文献/References:

[1] 陈祥荣，李亚松，骆良钦，等. 丙戊酸对大鼠创伤性颅脑损 伤后脑水肿和神经功能的影响[J]. 中华实验外科杂志， 2016，33（11）：2538-2542.
[2] 方永军，韦鹏方，周　锋，等. 阿托伐他汀治疗慢性硬膜下 血肿的临床观察[J]. 中国临床神经外科杂志，2018，23 （4）：39-40.
[3] Xu X, Gao W, Cheng S, et al. Anti-inflammatory and im- munomodulatory mechanisms of atorvastatin in a murine model of traumatic brain injury [J]. J Neuroinflammation, 2017, 14(1): 167-181.
[4] 李迪彬，涂　悦，程世翔，等. 亚低温联合促红细胞生成素 对颅脑创伤大鼠脑保护作用的研究[J]. 中华神经外科杂 志，2015，31（4）：381-385.
[5] 刘　亭，刘香香，陈亭亭，等. 灯盏细辛和赤芍配伍组方对 大鼠脑缺血再灌注损伤的保护作用及对NF-κB通路的 影响[J]. 中国实验方剂学杂志，2018，24（9）：111-115.
[6] 金卫篷，周　源，王　东，等. 阿托伐他汀对脑外伤小鼠血 脑屏障的影响[J]. 中华实验外科杂志，2015，32（6）： 1361-1364.
[7] 杨会杰，王金兰，乐　婷，等. 阿托伐他汀对局灶性脑缺血 大鼠脑水肿的影响及机制探讨[J]. 中华神经医学杂志， 2016，15（2）：130-135.
[8] 马　涛. 颅脑损伤后凝血功能障碍的机制及干预措施[J]. 中国临床神经外科杂志，2017，22（8）：601-603.
[9] Wang YL, He H, Liu ZJ, et al. Effects of TNF-α on cemen- toblast differentiation, mineralization, and apoptosis [J]. J Dent Res, 2015, 94(9): 1225-1232.
[10] Chu X, Wang H, Jiang YM, et al. Ameliorative effects of tannic acid on carbon tetrachloride-induced liver fibrosis invivo and invitro [J]. J Pharmacol Sci, 2016, 130(1): 15-23.
[11] 史晓静，王高频，陶贵周. 阿托伐他汀通过抑制NF-κB对 抗大鼠缺血再灌注心肌炎症反应和凋亡的机制[J]. 临床 心血管病杂志，2015，31（7）：788-790.
[12] 王成雅，陈立杰，黄　山，等. Toll样受体配体与脑缺血损 伤机制的研究进展[J]. 中国临床神经科学，2016，24（4）： 446-452..
[13] 杨雪华，王　锐，陈立杰，等. Toll样受体4信号通路对脑 缺血损伤影响的研究进展[J]. 中国临床神经科学，2016， 24（4）：470-474.
[14] Li G, Wu X, Yang L, et al. TLR4-mediated NF-κB signa- ling pathway mediates HMGB1-induced pancreatic injury in mice with severe acute pancreatitis [J]. Int J Mol Med, 2016, 37(1): 99-107.
[15] 王锦鹏，田丰永，徐　涛，等. 丹参酮ⅡA磺酸钠联合阿托 伐他汀对冠心病患者外周血单个核细胞TLR4、MyD88、 NF-κB表达的影响[J]. 河北医药，2016，38（1）：16-19.

相似文献/References:

[1]张善纲 综述.现代战争中颅脑损伤的特点及功能康复[J].中国临床神经外科杂志,2015,(11):701.[doi:10.13798/j.issn.1009-153X.2015.11.021]
[2]钟秀均　黄书岚.复方醒脑液治疗颅脑损伤后综合征的临床观察[J].中国临床神经外科杂志,2016,(05):278.[doi:10.13798/j.issn.1009-153X.2016.05.007]
　ZHONG Xiu-jun,HUANG Shu-lan..Clinical observation on treatment of compound decoction for cerebral prorection for post-traumatic brain syndrome[J].,2016,(04):278.[doi:10.13798/j.issn.1009-153X.2016.05.007]
[3]李国亮　邸　方　杨亚东.香芹酚通过抑制脑水肿与氧化应激反应保护大鼠 颅脑损伤[J].中国临床神经外科杂志,2016,(05):283.[doi:10.13798/j.issn.1009-153X.2016.05.009]
　LI Guo-liang,DI Fang,YANG Ya-dong..Carvacrol protects against traumatic brain injury in rats through suppressing the brain edma and oxidative stress[J].,2016,(04):283.[doi:10.13798/j.issn.1009-153X.2016.05.009]
[4]罗心凯　陈治标　陈谦学.免缝胶原海绵人工硬脑膜在颅脑损伤大骨瓣减压术中的应用[J].中国临床神经外科杂志,2016,(06):357.[doi:10.13798/j.issn.1009-153X.2016.06.012]
　LUO Xin-kai,CHEN Zhi-biao,CHEN Qian-xue..Application of collagen sponge artificial dura without suturing to big bone flap decompression after traumatic brain injury[J].,2016,(04):357.[doi:10.13798/j.issn.1009-153X.2016.06.012]
[5]贺瑛福　肖宗宇　马进海　许常林　裴　杰　袁　岗　张广华　 巨　虎.高海拔地区重型颅脑损伤血清FSH、LH、PRL的变化[J].中国临床神经外科杂志,2016,(06):359.[doi:10.13798/j.issn.1009-153X.2016.06.013]
　HE Ying-fu,XIAO Zong-yu,MA Jin-hai,et al.Changes in serum FSH, LH and PRL levels in the patients with severe traumatic brain injury in plateau[J].,2016,(04):359.[doi:10.13798/j.issn.1009-153X.2016.06.013]
[6]满明昊　李立宏　杨彦龙　李　敏　郭少春.颅脑损伤术后细胞免疫指标变化及脾多肽对其影响[J].中国临床神经外科杂志,2016,(06):362.[doi:10.13798/j.issn.1009-153X.2016.06.014]
　MAN Ming-hao,LI Li-hong,YANG Yan-yong,et al.Changes in peripheral cells immunity after operation and effect of spleen polypeptide on them in patients with traumatic brain injury[J].,2016,(04):362.[doi:10.13798/j.issn.1009-153X.2016.06.014]
[7]程　勇　秦加新　戢翰升　李　刚　聂劲林　李　强.颅脑损伤术中急性脑膨出的相关因素分析[J].中国临床神经外科杂志,2016,(06):374.[doi:10.13798/j.issn.1009-153X.2016.06.019]
[8]张一帆 综述　张国来 审校.弥漫性轴索损伤影像学诊断的研究进展[J].中国临床神经外科杂志,2016,(07):442.[doi:10.13798/j.issn.1009-153X.2016.07.020]
[9]陈吉钢　张丹枫　魏嘉良　邹　伟　侯立军.创伤性眶上裂综合征的治疗陈吉钢[J].中国临床神经外科杂志,2016,(04):200.[doi:10.13798/j.issn.1009-153X.2016.04.003]
　CHEN Ji-gang,ZHANG Dan-feng,WEI Jiang-liang,et al.Management of traumatic superior orbital fissure syndrome (report of 22 cases)[J].,2016,(04):200.[doi:10.13798/j.issn.1009-153X.2016.04.003]
[10]肖连福　黄瑞宏　陈昌勇.外伤性颅内静脉窦血栓形成1例[J].中国临床神经外科杂志,2016,(04):256.[doi:10.13798/j.issn.1009-153X.2016.04.024]

备注/Memo

备注/Memo:
（2019-07-09收稿，2019-09-29修回）
更新日期/Last Update: 2020-04-10