[1]李　鑫　刘振杰　梁　良　胡耀文　武力伟　解　靖　董海青.lncRNA LINC00689靶向调控miRNA-634促进胶质瘤U251细胞增殖、侵袭和迁移[J].中国临床神经外科杂志,2020,(12):851-854.[doi:10.13798/j.issn.1009-153X.2020.12.011]
　LI Xin,LIU Zhen-jie,LIANG Liang,et al.LncRNA LINC00689 targeting miRNA-634 promotes proliferation, invasion and migration of glioma U251 cells[J].,2020,(12):851-854.[doi:10.13798/j.issn.1009-153X.2020.12.011]
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lncRNA LINC00689靶向调控miRNA-634促进胶质瘤U251细胞增殖、侵袭和迁移()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2020年12期
页码:
851-854
栏目:
实验研究
出版日期:
2020-12-25

文章信息/Info

Title:
LncRNA LINC00689 targeting miRNA-634 promotes proliferation, invasion and migration of glioma U251 cells
文章编号:
1009-153X（2020）12-0851-03
作者:
李　鑫　刘振杰　梁　良　胡耀文　武力伟　解　靖　董海青
071000 河北，保定市第一中心医院神经外一科（李　鑫、刘振杰、梁　良、胡耀文 武力伟、解靖、董海青）
Author(s):
LI Xin;  LIU Zhen-jie;  LIANG Liang;  HU Yao-wen;  WU Li-wei;  JIE Jing;  DONG Hai-qing.
Department of Neurosurgery, Baoding First Central Hospital, Baoding 071000, China
关键词:
长链基因间非蛋白编码RNA689; 微小RNA-634; 胶质瘤
Keywords:
Glioma;  Long intergenic non-protein coding RNA 689;  MicroRNA-634;  LINC00689;  miRNA-634
分类号:
R 739.41; Q 786
DOI:
10.13798/j.issn.1009-153X.2020.12.011
文献标志码:
A
摘要:
目的 探讨长链基因间非蛋白编码RNA689（LINC00689）对胶质瘤细胞增殖、侵袭和迁移的影响。方法 RT-PCR实验检测正常胶质细胞HEB、胶质瘤细胞系A172、U251、U87、SHG-4中LINC00689和miRNA-634的表达。将si-NC、si- LINC00689、miRNA-634 mimics、miRNA-NC、si- LINC00689+anti-miRNA-NC、si- LINC00689+anti-miRNA-634质粒转染至U251细胞中；CCK-8实验检测细胞增殖、Transwell实验检测细胞侵袭和迁移。结果 与正常胶质细胞HEB比较，胶质瘤细胞系A172、U251、U87和SHG-4的LINC00689表达水平较高，而miRNA-634表达水平较低（P<0.05）。与si-NC组比较，si-LINC00689组LINC00689表达水平、细胞增殖活力、侵袭和迁移细胞数明显较低（P<0.05）。与miRNA-NC组比较，miRNA-634 mimics组miRNA-634表达水平明显增高（P<0.05），细胞增殖活力、侵袭和迁移细胞数明显降低（P<0.05）。与si- LINC00689+anti-miRNA-NC组比较，si- LINC00689+anti-miRNA-634组细胞增殖活力、侵袭和迁移细胞数明显增高（P<0.05）。结论 LINC00689可以促进胶质瘤细胞的增殖、侵袭和迁移，其机制可能与靶向调控miRNA-634有关。
Abstract:
Objective To investigate the effect of long intergenic non-protein coding RNA 689 (LINC00689) on the proliferation, invasion and migration of glioma U251 cells. Methods RT-PCR was used to detect the expression of LINC00689 and miRNA-634 in normal glioma HEB cells, and glioma cell lines A172, U251, U87 and SHG-4. The si-NC, si-LINC00689, miRNA-634 mimics, miRNA-NC, si-LINC00689+anti-miRNA-NC, si-LINC00689+anti-miRNA-634 plasmids were transfected into U251 cells, respectievly. CCK-8 test was used to detect the cell proliferation. Transwell test was used to detect the cell invasion and migration. Results Compared with HEB normal glial cells, the expression levels of LINC00689 in glioma cell lines A172, U251, U87 and SHG-4 were significantly increased (P<0.05), while the expression levels of miRNA-634 were significantly decreased (P<0.05). Compared with the si-NC group, the expression level of LINC00689, and the abilities of cell proliferation activity, invasion and migration were significantly decreased in si-LINC00689 group (P<0.05). Compared with the miRNA-NC group, the expression level of miRNA-634 signifcantly increased and the abilities of cell proliferation activity, invasion and migration were significantly decreased in the miRNA-634 mimics group (P<0.05). Compared with the si-LINC00689+anti-miRNA-NC group, the abilities of cell proliferation activity, invasion and migration were significantly increased in si-LINC00689+anti-miRNA-634 group (P<0.05). Conclusion LINC00689 can promote the proliferation, invasion and migration of glioma U251 cells, which may be related to the targeted regulation of miRNA-634.

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更新日期/Last Update: 2020-12-25