[1]娄志刚　袁　波　谭占国.STIP1对胶质瘤U251细胞增殖、侵袭和凋亡的影响[J].中国临床神经外科杂志,2020,(12):847-850.[doi:10.13798/j.issn.1009-153X.2020.12.010]
　LOU Zhi-gang,YUAN Bo,TAN Zhan-guo..Effects of STIP1 on proliferation, invasion and apoptosis of glioma U251 cells[J].,2020,(12):847-850.[doi:10.13798/j.issn.1009-153X.2020.12.010]
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STIP1对胶质瘤U251细胞增殖、侵袭和凋亡的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2020年12期
页码:
847-850
栏目:
实验研究
出版日期:
2020-12-25

文章信息/Info

Title:
Effects of STIP1 on proliferation, invasion and apoptosis of glioma U251 cells
文章编号:
1009-153X（2020）12-0847-04
作者:
娄志刚　袁　波　谭占国
462000 河南,漯河市中心医院神经外科( 娄志刚、袁　波、谭占国)
Author(s):
LOU Zhi-gang;  YUAN Bo;  TAN Zhan-guo.
Department of Neurosurgery, Luohe Central Hospital, Luohe 462000, China
关键词:
胶质瘤; U251细胞; 磷酸化应激诱导蛋白1; JAK2/STAT3信号通路; 细胞增殖; 细胞侵袭; 细胞凋亡; 基因沉默
Keywords:
Glioma;  U251 cells;  Stress-induced phosphoprotein 1;  Cell proliferation;  Cell invasion;  Cell apoptosis
分类号:
R 739.41; Q 786
DOI:
10.13798/j.issn.1009-153X.2020.12.010
文献标志码:
A
摘要:
目的 探讨下调磷酸化应激诱导蛋白1（STIP1）表达对胶质瘤U251细胞增殖、侵袭和凋亡的影响，及其对JAK2/STAT3信号通路的调控作用。方法 免疫印迹法检测体外培养的正常胶质细胞（SVG）和人胶质瘤细胞（U251、U87和U37）STIP1蛋白表达水平。NC-siRNA或STIP1-siRNA质粒转染U251细胞，CCK-8法检测U251细胞增殖；Transwell实验检测U251细胞侵袭能力；流式细胞术检测U251细胞凋亡率；免疫印迹法检测JAK2/STAT3信号通路蛋白表达水平。结果 与正常胶质细胞SVG比较，胶质瘤细胞U251、U87和U373的STIP1蛋白表达水平均明显增高（P<0.05）。与NC-siRNA组比较，STIP1-siRNA组STIP1蛋白表达水平、细胞增殖活力和细胞侵袭力明显明显降低（P<0.05），细胞凋亡率明显增高（P<0.05），而且，p-JAK2和p-STAT3蛋白表达水平明显降低（P<0.05）。结论 STIP1在胶质瘤细胞中呈高表达，抑制STIP1表达可以抑制胶质瘤细胞的增殖和侵袭、促进凋亡，机制可能与抑制JAK2/STAT3信号通路有关。
Abstract:
Objective To investigate the effect of stress-induced phosphoprotein 1 (STIP1) on the proliferation, invasion and apoptosis of glioma U251 cells. Methods Western-blot test was used to detect the expression level of STIP1 protein in normal glioma cells (SVG) and human glioma cell lines U251, U87 and U37. The NC-siRNA STIP1-siRNA plasmids were transfected into U251 cells, respectively. CCK-8 method was used to detect cell proliferation. Transwell test was used to detect cell invasion. Annexin-V-FITC/PI flow cytometry was used to detect cell apoptosis. Western-blot test was used to detect the protein expression levels of STIP1, JAK2, p-JAK2, STAT3 and p-STAT3. Results Compared with normal glioma SVG, the expression levels of STIP1 protein in glioma cell lines U251, U87 and U373 significantly increased (P<0.05). Compared with the NC-siRNA group, the expression levels of STIP1, p-JAK2 and p-STAT3, and the abilities of cell proliferation activity, cell invasiveness significantly decreased (P<0.05), while the cell apoptosis rate significantly increased (P<0.05). Conclusion STIP1 is highly expressed in glioma cells. Inhibiting STIP1 expression can inhibit the proliferation and invasion of glioma cells, promote cell apoptosis, which may be realted to inhibit the JAK2 / STAT3 signaling pathway.

参考文献/References:

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更新日期/Last Update: 2020-12-25