[1]汪超甲　王　辉　胡钧涛.RNAi对U251细胞c-Met基因表达水平的影响[J].中国临床神经外科杂志,2015,(02):99-100.[doi:10.13798/j.issn.1009-153X.2015.02.011]
　WANG Chao-jia,WANG Hui,HU Jun-tao..Effect of RNA interference on expression of c-Met gene in U251 cells[J].,2015,(02):99-100.[doi:10.13798/j.issn.1009-153X.2015.02.011]
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RNAi对U251细胞c-Met基因表达水平的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2015年02期
页码:
99-100
栏目:
论著
出版日期:
2015-02-28

文章信息/Info

Title:
Effect of RNA interference on expression of c-Met gene in U251 cells
文章编号:
1009-153X（2015）02-0099-02
作者:
汪超甲　王　辉　胡钧涛
442000 湖北十堰，湖北医药学院附属太和医院神外科
通讯作者：王　辉，E-mail：sjwk713@163.com
Author(s):
WANG Chao-jia;  WANG Hui;  HU Jun-tao.
Department of Neurosurgery, Affliated Taihe Hospital, Hubei Medicine College, Shiyan 442000, China
关键词:
胶质瘤; U251细胞; c-Met基因; RNA干扰
Keywords:
U251 cells;  C-Met genes;  Transfection;  RNA interference
分类号:
R 739.41; Q 786
DOI:
10.13798/j.issn.1009-153X.2015.02.011
文献标志码:
A
摘要:
目的 探讨RAN干扰作用对人脑胶质瘤U251细胞c-Met基因表达的影响。方法 设计3对以c-Met为靶基因短发夹RNA片段，pGenesil-1质粒为载体,构建pGenesil-1/c-Met-shRNA重组质粒，将其转染人脑胶质瘤U251细胞。采用PCR和免疫印迹法分别检测c-Met基因mRNA和蛋白的表达水平。结果 成功构建pGenesil-1-c-Met shRNA重组质粒，并转染至U251细胞。转染重组质粒的U251细胞c-Met基因mRNA和蛋白表达水平均显著降低（P<0.05）。结论 RNA干扰能明显降低U251细胞c-Met基因mRNA和蛋白的表达水平。
Abstract:
Objective To construct the shRNA expression vector which targeted c-Met gene and explore the effect of the vector on the expression levels of c-Met gene in the U251 cells. Methods Three pairs of pGenesil-1-c-Met-shRNA recombinant plasmids were constructed. The recombinant plasmids were transfected into the U251 cells. The c-Met mRNA and protein expression levels were determined respectively by the RT-PCR and Western blot methods. Results The pGenesil-1-c-Met shRNA recombinant plasmids were successfully constructed and transfected into U251 cells. The levels of c-Met mRNA expression and protein expression were significantly lower in the U251 cells transfected with pGengsil-1-c Met shRNA recombinant plasmids than those in the U251 cells untransfected with the recombinant plasmids (P<0.05). Conclusion RNA interference can significantly reduce c-Met mRNA and protein expression level in the U251 cells.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：湖北省教育厅青年人才项目（Q20112103）
更新日期/Last Update: 2015-02-28