[1]续继军　孟　铸　李志鹏　陈　欣　任庆先　秦　汉.胶质瘤细胞GDNF基因启动子Ⅰ区甲基化水平对其基因转录的影响[J].中国临床神经外科杂志,2015,(07):410-412.[doi:10.13798/j.issn.1009-153X.2015.07.009]
　XU Ji-jun,MENG Zhu,LI Zhi-peng,et al.Effect of methylation levels of GDNF gene promoter Ⅰ region on its transcription in glioma U251 cells[J].,2015,(07):410-412.[doi:10.13798/j.issn.1009-153X.2015.07.009]
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胶质瘤细胞GDNF基因启动子Ⅰ区甲基化水平对其基因转录的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2015年07期
页码:
410-412
栏目:
论著
出版日期:
2015-07-30

文章信息/Info

Title:
Effect of methylation levels of GDNF gene promoter Ⅰ region on its transcription in glioma U251 cells
文章编号:
1009-153X（2015）07-0410-03
作者:
续继军　孟　铸　李志鹏　陈　欣　任庆先　秦　汉
277500 山东，滕州市中医医院神经外科（续继军、孟　铸、李志鹏、陈　欣、任庆先）；430070 武汉，广州军区武汉总医院神经外科（秦　汉）
Author(s):
XU Ji-jun1;  MENG Zhu1;  LI Zhi-peng1;  CHEN Xin1;  REN Qing-xian1;  QIN Han2.
1. Department of Neurosurgery, Traditional Chinese Medical Hospital of Tengzhou City, Tengzhou 277500, China; 2. Department of Neurosurgery, Wuhan General Hospital, Guangzhou Command, PLA, Wuhan 430070, China
关键词:
胶质瘤; U251细胞; 胶质细胞原性神经营养因子; 启动子; 甲基化; 基因转录
Keywords:
Glioma;  GDNF;  U251 cells;  Promoter;  Methylation;  Expression
分类号:
R 739.41; Q 786
DOI:
10.13798/j.issn.1009-153X.2015.07.009
文献标志码:
A
摘要:
目的 探讨胶质瘤细胞胶质细胞原性神经营养因子（GDNF）基因启动子Ⅰ区甲基化水平对其基因转录的影响。方法 体外培养胶质瘤U251细胞，加入不同浓度5-氮杂胞苷（浓度分别为1、5、10和20 μmol/L）干预，以加入PBS为对照。采用重亚硫酸盐测序法测定GDNF基因启动子Ⅰ区甲基化水平，RT-PCR检测GDNF mRNA的表达。结果 与PBS组相比，1 μmol/L 5-氮杂胞苷对GDNF基因启动子Ⅰ区甲基化水平无显著影响（P>0.05），5、10和20 μmol/L 5-氮杂胞苷均显著降低其甲基化水平（P<0.05）。与PBS组相比，1 μmol/L 5-氮杂胞苷对GDNF mRNA表达水平无显著影响（P>0.05），5 μmol/L 5-氮杂胞苷显著增加其表达水平（P<0.05），但随着浓度进一步增加（10、20 μmol/L），其表达水平逐渐降低。结论 5-氮杂胞苷对GDNF基因具有去甲基化作用；GDNF启动子Ⅰ区去甲基化能够增加GDNF基因的转录水平。
Abstract:
Objective To explore the effect of methylation levels of glia cell line-derived neurotroplic factor (GDNF) promoter Ⅰregion on its transcription in glioma U251 cells. Methods The methylation levels of GDNF promoter Ⅰ region were detected before and after intervention with different concentrations of 5-aza-CR in glioma U251 cells. The GDNF mRNA expression was detected by RT-PCR in each group. Results The methylation levels of GDNF gene promoter Ⅰ region declined after the intervention with 5-aza-cR compared to the control group. The methylation levels of GDNF gene promoter Ⅰ region were significantly lower in 5 μmol, 10 μmol and 20 μmol 5-aza-CR intervention groups than that in the control group (P<0.05). The level of GDNF mRNA expression in U251 cells was significantly higher in the 5 μmol 5-aza-CR intervention group than those in the control group and 1 μmol, 10 μmol and 20 μmol intervention groups (P<0.05). There were insignificant differences in the levels of GDNF mRNA expression in U251 cells among the control group and 1 μmol, 10 μmol and 20 μmol 5-aza-CR intervention groups (P>0.05). Conclusion The demethylation of GDNF gene promoter Ⅰ region, which is produced by 5-aza-CR, may influence the expression of GDNF in U251 cell.

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更新日期/Last Update: 2015-07-30