[1]潘智勇　徐成仕　王泽芬　马　超　王　舒　李志强.大鼠不同程度颅脑损伤模型的建立及评估[J].中国临床神经外科杂志,2015,(07):413-415,418.[doi:10.13798/j.issn.1009-153X.2015.07.010]
　PAN Zhi-yong,XU Cheng-shi,WANG Ze-fen,et al.Establishment and evaluation of graded traumatic brain injury models in rats[J].,2015,(07):413-415,418.[doi:10.13798/j.issn.1009-153X.2015.07.010]
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大鼠不同程度颅脑损伤模型的建立及评估()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2015年07期
页码:
413-415,418
栏目:
论著
出版日期:
2015-07-30

文章信息/Info

Title:
Establishment and evaluation of graded traumatic brain injury models in rats
文章编号:
1009-153X（2015）07-0413-03
作者:
潘智勇　徐成仕　王泽芬　马　超　王　舒　李志强
430071 武汉，武汉大学中南医院神经外科（潘智勇、徐成仕、马　超、李志强）；430071 武汉，武汉大学基础医学院生理系（王泽芬、王　舒）
通讯作者：李志强，E-mail：lifenzhi@163.com
Author(s):
PAN Zhi-yong1;  XU Cheng-shi1;  WANG Ze-fen2;  MA Chao1;  WANG Shu2;  LI Zhi-qiang1.
1. Department of Neurosurgery, Zhongnan Hospital, Wuhan University, Wuhan 430071, China; 2. Department of Physiology, Basic Medical School, Wuhan University, Wuhan 430071, China
关键词:
颅脑损伤; 动物模型; 大鼠
Keywords:
Traumatic brain injury;  Animal model;  Rats;  Evaluation
分类号:
R 651.1+5; R -331
DOI:
10.13798/j.issn.1009-153X.2015.07.010
文献标志码:
A
摘要:
目的 探讨大鼠不同程度颅脑损伤（TBI）模型的制作方法。方法 将40只SD大鼠随机分为4组：对照组、轻型TBI组、中型TBI组及重型TBI组，每组10只。采用Feeney法自由落体颅脑损伤装置并进行适当改进，以不同致伤冲击力（200、600和1 000 g/cm）建立轻、中、重型TBI模型，对照组仅去骨瓣，不致脑损伤。伤后24 h观察各组大鼠行为学及病理组织学改变。结果 与对照组相比，行为学结果显示，随着致伤冲击力增加，平衡木试验得分越高（P<0.05）、神经损害程度评分越高（P<0.05）、水迷宫试验中逃避潜伏期越长（P<0.05）且经过平台次数越少（P<0.05）。组织病理学发现损伤程度及范围随致伤冲击力的增大而增加。结论 本研究参照Feeney法自由落体TBI装置并适当给予改进，成功建立不同程度大鼠TBI模型，其制作方法简单、可控性好、可重复性好，可用于TBI的动物实验研究。
Abstract:
Objective To set up and evaluate the graded traumatic brain injury (TBI) in rats. Methods Forty SD rats were divided into 4 groups of 10 animals each, i.e. control group (no injury), mild injury group, moderate injury group and severe injury group. The graded TBI models were established by the modified Feeney's method. The changes in the behavior and cerebral tissues pathology were observed 24 hours after the injury in all the groups. Results The more severe TBI was, the worse the coordinated ability of action (balance wood test) was, the higher the neurological severity scores were, the worse the learning and memory (water maze test) were and the more serious the pathological changes in the cerebral tissues were. Conclusion The model of graded TBI is successful established, and may be applied to the experimental study of TBI.

参考文献/References:

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更新日期/Last Update: 2015-07-30