[1]乔战东　刘雷震　贾利云　李经纶　刘　伟　曹付强　姚安会　王本瀚.人脑挫裂伤后早期小胶质细胞PD-L1的表达[J].中国临床神经外科杂志,2016,(04):216-218.[doi:10.13798/j.issn.1009-153X.2016.04.008]
　QIAO Zhan-dong,LIU Lei-zhen,et al.PD-L1 expression in microglial cells early after contusion and laceration of human brain[J].,2016,(04):216-218.[doi:10.13798/j.issn.1009-153X.2016.04.008]
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人脑挫裂伤后早期小胶质细胞PD-L1的表达()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2016年04期
页码:
216-218
栏目:
论著
出版日期:
2016-04-25

文章信息/Info

Title:
PD-L1 expression in microglial cells early after contusion and laceration of human brain
文章编号:
1009-153X（2016）04-0216-03
作者:
乔战东　刘雷震　贾利云　李经纶　刘　伟　曹付强　姚安会　王本瀚
450042 郑州，解放军第153中心医院神经外科（乔战东、刘雷震、 贾利云、李经纶、刘　伟、曹付强、姚安会、王本瀚）；450001 郑州，郑州大学基础医学院医学遗传与细胞生物学教研室（贾利云）
通讯作者：王本瀚，E-mail：wangbenhan@sina.com
Author(s):
QIAO Zhan-dong1;  2;  LIU Lei-zhen1;  2;  JIA Li-yun3;  LI Jing-lun1;  LIU Wei1;  CAO Fu-qiang1;  YAO An-hui1;  WANG Ben-han1.
1, Department of Neurosurgery, The 153rd Central Hospital, PLA, Zhengzhou 450042, China; 2, Xinxiang Medical School, Xinxiang 453003, China; 3. Department of Medical Genetics and Cell Biology, Zhengzhou University, Zhengzhou 450001, China
关键词:
脑挫裂伤; 小胶质细胞; 细胞程序性细胞死亡因子1配体; 炎症反应
Keywords:
Brain contusion and laceration;  Microglial cell;  Programmed death-1 ligand;  Expression
分类号:
R 651.1+5; Q 786
DOI:
10.13798/j.issn.1009-153X.2016.04.008
文献标志码:
A
摘要:
目的 探讨人脑挫裂伤后早期小胶质细胞程序性细胞死亡因子1配体（PD-L1）的表达变化。方法 收集2013~2015年手术治疗的脑挫裂伤组织标本15例，其中伤后6 h 5例，伤后12 h 4例，伤后1 d 3例，伤后4 d 3例；另选择3例肿瘤全切术中切除颞极组织作为对照组。采用HE染色及免疫荧光染色分析炎症细胞浸润情况，以及小胶质细胞PD-L1的表达情况。结果 HE染色结果显示，与对照组脑组织相比，脑挫裂伤后6、12 h未见到明显的炎症细胞浸润；随着损伤时间的延长，浸润的来自血液中的炎症细胞明显增加（P<0.05）。免疫荧光染色>结果显示，与对照组脑组织相比，脑挫裂伤后6 h表达PD-L1的小胶质细胞阳性细胞数明显升高（P<0.01）；随着损伤时间的延长，表达pd-l1的小胶质细胞阳性细胞数进一步升高（>P<0.05）。>结论 PD-L1可能参与了人脑挫裂伤后的炎症反应。
Abstract:
Objective To explore the changes in programmed death-1 ligand (PD-L1) expression in microglia cells early after contusion and laceration of human brain. Methods Eighteen cerebral tissues specimens, of which 3 were normal cerebral tissues, 5, 4, 3 and 3 were contused and lacerated cerebral tissues 6 hours, 12 hours, 1 day and 4 days after the brain injury respectively, were fixed by perfusion of 4% paraformaldehyde. Frozen sections were made for H-E and immunofluoresent staining and then the number of infiltrating inflammatory cells and the expression of PD-L1 in the microglial cells were determined. Results The Results of H-E staining showed that the number of infiltrating inflammatory cells in the contused and lacerated cerebral tissues 1 day after injury was significantly more than those in the normal cerebral tissues (P<0.05), and="" the="" number="" of="" infiltrating="" inflammatory="" cells="" significantly="" increased="" as="" time="" after="" injury="" (P<0.05). the="">Results of immunofluoresent staining that the number of PD-L1 positive microglial cells in the contused and lacerated cerebral tissues 6 hours after injury was significantly more than those in the normal cerebral tissues (P<0.05), and="" the="" number="" of="" pd-l1="" positive="" microglial="" cells="" significantly="" increased="" as="" time="" after="" injury="" (P<0.05).>Conclusions It is suggested that PD-L1 may be involved in the inflammation after human cerebral contusion and laceration and the intervention of the PD-1/PD-L1 signaling pathway may be a new Method to treat the human cerebral contusion and laceration.

参考文献/References:

[1] Faul M, Coronado V. Epidemiology of traumatic brain injury [J]. Handb Clin Neurol, 2015, 127: 3-13.
[2] Williams OH, Tallantyre EC, Robertson NP. Traumatic brain injury: pathophysiology, clinical outcome and treatment [J]. J Neurol, 2015, 262(5): 1394-1396.
[3] Chan CC. Inflammation: beneficial or detrimental after spinal cord injury [J]? Recent Pat CNS Drug Discov, 2008, 3 (3): 189-199.
[4] Keir ME, Butte MJ, Freeman GJ, et al. PD-1 and its ligands in tolerance and immunity [J]. Annu Rev Immunol, 2008, 26: 677-704.
[5] Yao A, Liu F, Chen K, et al. Programmed death 1 deficiency induces the polarization of macrophages/microglia to the M1 phenotype after spinal cord injury in mice [J]. Neurothera- peutics, 2014, 11(3): 636-650.
[6] Li P, Mao L, Liu X, et al. Essential role of program death 1-ligand 1 in regulatory T-cell-afforded protection against blood-brain barrier damage after stroke [J]. Stroke, 2014, 45 (3): 857-864.
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更新日期/Last Update: 2016-04-30