[1]唐　铸　李延良　桂　铮　张鹏飞　赵立文　姬文婕　于耀宇.甘草酸二铵对大鼠脑缺血-再灌注损伤的保护作用[J].中国临床神经外科杂志,2016,(05):287-289.[doi:10.13798/j.issn.1009-153X.2016.05.010]
　TANG Zhu,LI Yan-liang,GUI Zheng,et al.Diammonium glycyrrhizinate attenuates cerebral ischemia-reperfusion injury by Akt/caspase-3 pathway in rats[J].,2016,(05):287-289.[doi:10.13798/j.issn.1009-153X.2016.05.010]
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甘草酸二铵对大鼠脑缺血-再灌注损伤的保护作用()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2016年05期
页码:
287-289
栏目:
论著
出版日期:
2016-05-25

文章信息/Info

Title:
Diammonium glycyrrhizinate attenuates cerebral ischemia-reperfusion injury by Akt/caspase-3 pathway in rats
文章编号:
1009-153X（2016）05-0287-03
作者:
唐　铸　李延良　桂　铮　张鹏飞　赵立文　姬文婕　于耀宇
作者单位：063009 河北唐山，华北理工大学研究生院（唐　铸）；300162 天津，武警后勤学院附属医院神经外科（李延良、桂　铮、张鹏飞、赵立文、姬文婕、于耀宇）
通讯作者：于耀宇，E-mail：yuyaoyu666@aliyun.com
Author(s):
TANG Zhu1;  LI Yan-liang2;  GUI Zheng2;  ZHANG Peng-fei2;  ZHAO Li-wen2;  JI Wen-jie2;  YU Yao-yu2.
1. Gratuate School, North China University of Science and Technology, Tangshan 063000, China; 2. Department of Neurosurgery, Affiliated Hospital, Logistics University of People's Armed Police Forces, Tianjin 300162, China
关键词:
脑缺血-再灌注损伤; 细胞凋亡; 甘草酸二铵; 脑保护作用; 大鼠
Keywords:
Cerebral ischemical-reperfusion injury;  Cell apoptosis;  Diammonium glycyrrhizinate;  Cerebral protection;  Rats
分类号:
R 739.41; R 651.1+1
DOI:
10.13798/j.issn.1009-153X.2016.05.010
文献标志码:
A
摘要:
目的 探讨甘草酸二铵对大鼠脑缺血-再灌注损伤的作用。方法 将30只雄性SD大鼠（体重为250~300 g）随机分为假手术组、甘草酸二铵组、模型组，每组10只。采用可逆性大脑中动脉线栓法制作大鼠局灶性脑缺血-再灌注损伤模型。采用Zea-Longa评分评估大鼠神经功能。运用尼氏体染色检测大鼠海马组织尼氏小体数量，免疫组化法检测大鼠海马组织Akt、caspase-3阳性细胞数量。结果 术后24 h甘草酸二铵组和模型组大鼠Zea-Longa评分明显高于假手术组（P<0.05）；造模后3 d，甘草酸二铵组神经功能较模型组明显改善（P<0.05）。甘草酸二铵组海马组织尼氏小体数量和Akt阳性细胞数量较模型组明显增加，而caspase-3阳性细胞数量较模型组明显减少（P<0.05）。结论 甘草酸二铵可通过Akt/caspase-3途径减轻脑缺血-再灌注损伤大鼠细胞凋亡，发挥对大鼠缺血-再灌注损伤的保护作用。
Abstract:
Objective To study the effect of diammonium glycyrrhizinate (DG) on cerebral ischemia-reperfusion injury in rats. Methods Thirty SD rats were randomly divided into 3 groups, i.e. sham operation group (n=10), model group (n=10) and DG treatment group (n=10). The rat model of focal cerebral ischemia-reperfusion injury was made by reversible middle cerebral artery occlusion in rats. The rats in DG traetment group received intraperitoneal injection of DG [20 mg/(kg.d), qd] for 3 days, and the rats in sham operation and model groups received intraperitoneal injection of indentical volume of physiological saline. Zea-longa score was used to assess the neurological function 2 hours and 3 days after the injury. The number of Nissl bodies of neurons was assess by Nissl's staining and the numbers of caspase-3 positive cells and Akt positive cells were detected by immunohistochemistry staining in the hippocampus tissues 3 days after the injury. Results Two hours after injury, the Zea-longa scores in DG treatment group [(2.60±0.51) points] and model group [(2.59±0.54) points] were significantly higher than that (zero point) in sham operation group (P<0.05), and there was no significant difference between DG treatment and model groups (P>0.05). Three days after injury, the Zea-longa score in DG treatment group [(1.90±0.74) points] was still significantly higher than that (zero point) in sham operation group (P<0.05), and significantly lower than that [(2.50±0.48) points] in model group (P<0.05). The numbers of Nissl bodies of neurons and Akt positive cells in DG treatment group were significantly more than that in model group (P<0.05), and the number of caspase-3 positive cells in DG treatment group was significantly fewer than that in model group (P<0.05). Conclusion It is suggested that DG can reduce the level of neuron apoptosis by Akt/caspase-3 pathway, thus protect the cerebral tissues after the cerebral ischemia-reperfusion injury in the rats.

参考文献/References:

[1] Lapi D, Colantuoni A. Remodeling of cerebral microcircu- lation after ischemia-reperfusion [J]. J Vasc Re, 2015, 52 (1): 22-31.
[2] Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebralartery occlusion without craniectomyinrats [J]. Stroke, 1989, 20(1): 84-91.
[3] Zhu Y, Liu F, Zou X, et al. Comparison of unbiased estima- tion of neuronalnumberin the rat hippocampus with different staining methods [J]. J Neurosci Methods, 2015, 30, 254: 73-79.
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更新日期/Last Update: 2016-05-30