[1]周德才　王少雄　黄　程等.miR-497对恶性脑膜瘤细胞增殖的影响[J].中国临床神经外科杂志,2021,26(02):106-109.[doi:10.13798/j.issn.1009-153X.2021.02.013]
　ZHOU De-cai,WANG Shao-xiong,HUANG Cheng,et al.Effect of miR-497 on proliferation of malignant meningioma cells[J].,2021,26(02):106-109.[doi:10.13798/j.issn.1009-153X.2021.02.013]
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miR-497对恶性脑膜瘤细胞增殖的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
26
期数:
2021年02期
页码:
106-109
栏目:
实验研究
出版日期:
2021-02-25

文章信息/Info

Title:
Effect of miR-497 on proliferation of malignant meningioma cells
文章编号:
1009-153X（2021）02-0106-04
作者:
周德才　王少雄　黄　程等
571100，海口市第三人民医院神经外科（周德才、王少雄、黄程）；541001 广西桂林，桂林医学院附属医院神经外科（王文波）
Author(s):
ZHOU De-cai;  WANG Shao-xiong;  HUANG Cheng;  et al
Department of Neurosurgery, Haikou Third People’s Hospital, Haikou 571100, China; 2. Department of Neurosurgery, Affiliated Hospital of Guilin Medical College, Guilin 541001, China
关键词:
恶性脑膜瘤; 细胞增殖; miR-497; 脂肪酸合成酶; FASN
Keywords:
Malignant meningioma;  miR-497;  Fatty acid synthase (FASN);  Cell proliferation
分类号:
R 739.41; Q 786
DOI:
10.13798/j.issn.1009-153X.2021.02.013
文献标志码:
A
摘要:
目的 探讨miR-497对恶性脑膜瘤细胞增殖的影响及作用机制。方法 收集2019年1~9月手术切除的女性脑膜瘤组织标本10例，其中恶性脑膜瘤5例（WHO分级Ⅲ级），良性脑膜瘤5例（WHO分级Ⅰ级），采用RNA-seq法和qRT-PCR检测脂肪酸合成酶（FASN）mRNA和miR-497表达水平。体外培养恶性脑膜瘤细胞株IOMM-Lee细胞，设置空白组（不做任何处理）、miR-NC组（转染negative control质粒）、miR-497-m组（转染miR-497 mimics）和miR-497-i组（转染miR-497 inhibitor），MTT法检测细胞增殖能力，检测细胞上清液乳酸水平和细胞ATP含量，免疫印迹法检测细胞FASN蛋白表达水平。结果 恶性脑膜瘤组织FASN mRNA水平明显高于良性脑膜瘤组织（P<0.05），而miR-497水平则明显低于良性脑膜瘤组织（P<0.05）。与空白组和miR-NC组相比，miR-497-m组细胞增殖能力、细胞上清液乳酸水平、细胞ATP含量以及FASN蛋白表达水平均明显降低（P<0.05）；而miR-497-i组细胞增殖能力、细胞上清液乳酸水平、细胞ATP含量以及FASN蛋白表达水平均明显升高（P<0.05）。结论 恶性脑膜瘤组织miR-497呈低表达，通过靶向促进FASN表达，提高肿瘤细胞内脂肪酸代谢水平，促进肿瘤细胞增殖。
Abstract:
Objective To investigate the effect of miR-497 on the proliferation of malignant meningioma cells and its mechanism.Methods The levels of fatty acid synthase (FASN) mRNA and miR-497 were detected by RNA-seq and qRT-PCR in meningioma tissues obtained from 10 female patients with meningioma including 5 malignant and 5 benign meningiomas. IOMM-Lee cells were cultured in vitro and divided into blank group, miR-NC group (transfected with negative control), miR-497-m group (transfected with miR-497 mimics) and miR-497-i group (transfected with miR-497 inhibitor). The cell proliferation ability was detected by MTT method; lactic acid in cell supernatant and cell ATP contents were detected; FASN protein expression were measured using western blotting. Results The mRNA level of FASN was signifcantly higher and the miR-497 level was signifcantly lower in malignant meningioma tissues than benign meningioma tissues (P<0.05). Compared with blank and miR-NC groups, the cell proliferation activity,lactate acid level in the cell supernatant, cell ATP content and FASN protein expression level significantly decreased in miR-497-m group (P<0.05), while significantly increased in miR-497-i group (P<0.05). Conclusion The expression level of miR-497 in malignant meningioma tissue is low, which may be by promoting FASN expression and increasing the fatty acid metabolism level in tumor cells to promote tumor cell proliferation.

参考文献/References:

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更新日期/Last Update: 2021-02-25