[1]付宏亮,景文记,卢俊义.血清miR-149-3p和CXCR3水平与aSAH后迟发性脑缺血的相关性分析[J].中国临床神经外科杂志,2023,28(01):11-14.[doi:10.13798/j.issn.1009-153X.2023.01.004]
　FU Hong-liang,JING Wen-ji,LU Jun-yi.Correlation of serum levels of miR-149-3p and CXCR3 with delayed cerebral ischemia after aneurysmal subarachnoid hemorrhage[J].,2023,28(01):11-14.[doi:10.13798/j.issn.1009-153X.2023.01.004]
点击复制

血清miR-149-3p和CXCR3水平与aSAH后迟发性脑缺血的相关性分析()
分享到：

《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
28
期数:
2023年01期
页码:
11-14
栏目:
论著
出版日期:
2023-01-25

文章信息/Info

Title:
Correlation of serum levels of miR-149-3p and CXCR3 with delayed cerebral ischemia after aneurysmal subarachnoid hemorrhage
文章编号:
1009-153X（2023）01-0011-04
作者:
付宏亮; 景文记; 卢俊义
041000山西，临汾市人民医院神经外科（付宏亮、景文记、卢俊义）
Author(s):
FU Hong-liang;  JING Wen-ji;  LU Jun-yi
Department of Neurosurgery, Linfen People's Hospital, Linfen 041000, China
关键词:
动脉瘤性蛛网膜下腔出血; 微小RNA-149-3p; CXC趋化因子受体3; 迟发性脑缺血
Keywords:
Aneurysmal subarachnoid hemorrhage;  MicroRNA-149-3p;  CXC chemokine receptor 3;  Delayed cerebral ischemia
分类号:
R743.9
DOI:
10.13798/j.issn.1009-153X.2023.01.004
文献标志码:
A
摘要:
目的 探讨血清微小RNA-149-3p（miR-149-3p）、CXC趋化因子受体3（CXCR3）与动脉瘤性蛛网膜下腔出血（aSAH）后迟发性脑缺血（DCI）的相关性。方法 2020年6月~2022年6月前瞻性收集aSAH共175例，采用qRT-PCR法检测血清miR-149-3p，采用酶联免疫吸附法检测血清CXCR3水平。结果 175例中，58例发生DCI，发生率为33.1%；117例未发生DCI。与未发生DCI病人比较，DCI病人血清miR-149-3p水平显著降低（P<0.05），CXCR3水平显著升高（P<0.05）。aSAH病人血清miR-149-3p水平与CXCR3水平呈明显负相关（r=-0.462，P<0.001）；多因素logsitic回归分析显示，miR-149-3p水平降低、CXCR3水平增高是aSAH后发生DCI的独立危险因素（P<0.05）。ROC曲线分析显示，血清miR-149-3p水平≤0.94预测aSAH后发生DCI的曲线下面积（AUC）为0.828，敏感度为82.76%，特异度为72.65%；血清CXCR3水平≥12.81 ng/L预测aSAH后发生DCI的AUC为0.812，敏感度为79.31%，特异度为83.76%；血清miR-149-3p≤0.94联合血清CXCR3联合≥12.81 ng/L预测aSAH后发生DCI的AUC为0.906，敏感度为91.38%，特异度为83.76%。两者联合预测效果更好（P<0.05）。结论 aSAH后发生DCI病人血清miR-149-3p水平降低、CXCR3水平升高，是aSAH后发生DCI独立危险因素，可能作为预测aSAH后发生DCI的指标。
Abstract:
Objective To investigate the correlation between serum levels of microRNA-149-3p (miR-149-3p) and CXC chemokine receptor 3 (CXCR3) and delayed cerebral ischemia (DCI) after aneurysmal subarachnoid hemorrhage (aSAH). Methods A total of 175 patients with aSAH were prospectively recruited from June 2020 to June 2022. Serum levels of miR-149-3p and CXCR3 were detected by qRT-PCR and enzyme-linked immunosorbent assay, respectively. Results Of these 175 patients, 58 patients had DCI (33.1%), and 117 did not. The incidence of DCI was 33.1%. Compared with patients without DCI, the serum level of miR-149-3p was significantly decreased (P<0.05) and the serum CXCR3 level was significantly increased (P<0.05) in patients with DCI. The serum level of miR-149-3p was significantly negatively correlated with the serum level of CXCR3 in aSAH patients (r=-0.462, P<0.001). Multivariate logsitic regression analysis showed that a decrease in serum level of miR-149-3p and an increase in serum level of CXCR3 were independent risk factors for DCI after aSAH (P<0.05). ROC curve analysis showed that the area under the curve (AUC) of serum miR-149-3p level ≤0.94 for predicting DCI after aSAH was 0.828, the sensitivity was 82.76%, and the specificity was 72.65%. The AUC of serum CXCR3 level ≥12.81 ng/L for predicting DCI after aSAH was 0.812, the sensitivity was 79.31%, and the specificity was 83.76%. The AUC of serum miR-149-3p ≤0.94 combined with serum CXCR3 ≥12.81 ng/L for predicting DCI after aSAH was 0.906, the sensitivity was 91.38%, and the specificity was 83.76%. The prediction effectiveness of two indexes was significantly better than that of a single index (P<0.05). Conclusions A decrease in serum level of miR-149-3p and an increase in serum level of CXCR3 are independent risk factors for DCI after aSAH, which may be used as indicators to predict DCI after aSAH.

参考文献/References:

[1]河南省医师协会神经介入专业委员会，河南省微循环学会神经重症分会. 《高分级动脉瘤性蛛网膜下腔出血综合救治》试行方案中原区域专家共识[J]. 中国临床神经外科杂志，2022，27（6）：513-521.
[2]陈　鹤，赵玉龙，刘佳明，等. 前交通动脉破裂动脉瘤术后认知功能障碍的影响因素[J]. 中国临床神经外科杂志，2022，27（5）：366-369.
[3]廖廷泉，苏祖禄，石　会. CT灌注成像预测及诊断蛛网膜下腔出血后迟发性脑缺血的Meta分析[J]. 中国临床神经外科杂志，2018，23（7）：458-462.
[4]Ikram A, Javaid MA, Ortega-Gutierrez S, et al. Delayed cerebral ischemia after subarachnoid hemorrhage [J]. J Stroke Cerebrovasc Dis, 2021, 30(11): 1-10.
[5]Tian Y, Jiang Y, Dong X, et al. miR-149-3p suppressed epithelial-mesenchymal transition and tumor development in acute myeloid leukemia [J]. Hematology, 2021, 26(1): 840-847.
[6]Vasudeva K, Munshi A. miRNA dysregulation in ischaemic stroke: focus on diagnosis, prognosis, therapeutic and protective biomarkers [J]. Eur J Neurosci, 2020, 52(6): 3610-3627.
[7]Hu X, Xiang Z, Zhang W, et al. Protective effect of DLX6-AS1 silencing against cerebral ischemia/reperfusion induced impairments [J]. Aging (Albany NY), 2020, 12(22): 23096-23113.
[8]Chaudhry SR, Kahlert UD, Kinfe TM, et al. Differential polarization and activation dynamics of systemic T helper cell subsets after aneurysmal subarachnoid hemorrhage (SAH) and during post-SAH complications [J]. Sci Rep, 2021, 11(1): 1-10.
[9]中华医学会神经病学分会，中华医学会神经病学分会脑血管病学组，中华医学会神经病学分会神经血管介入协作组. 中国蛛网膜下腔出血诊治指南2019[J]. 中华神经科杂志，2019，52（12）：1006-1021.
[10]Zafar SF, Westover MB, Gaspard N, et al. Interrater agreement for consensus definitions of delayed ischemic events after aneurysmal subarachnoid hemorrhage [J]. J Clin Neurophysiol, 2016, 33(3): 235-240.
[11]田　竺，胡群亮，王延民，等. 脑脊液气体分析与动脉瘤性蛛网膜下腔出血后迟发性脑缺血的相关性[J]. 中南医学科学杂志，2021，49（2）：169-172.
[12]Rumalla K, Lin M, Ding L, et al. Risk factors for cerebral vasospasm in aneurysmal subarachnoid hemorrhage: a population-based study of 8346 patients [J]. World Neurosurg, 2021, 145(1): e233-e241.
[13]Xing F, Liu Y, Dong R, et al. miR-374 improves cerebral ischemia reperfusion injury by targeting Wnt5a [J]. Exp Anim, 2021, 70(1): 126-136.
[14]王　娜，卞玉杰，田继新. 血清mir-210、HIF-1α水平与动脉瘤性蛛网膜下腔出血后迟发性脑缺血的关系[J]. 中风与神经疾病杂志，2020，37（4）：302-305.
[15]Cao Y, Wang Z, Yan Y, et al. Enterotoxigenic bacteroides-fragilis promotes intestinal inflammation and malignancy by inhibiting exosome-packaged miR-149-3p [J]. Gastroenterology, 2021, 161(5): 1552-1566.
[16]Jing R, Pan W, Long T, et al. LINC00472 regulates vascular smooth muscle cell migration and proliferation via regulating miR-149-3p [J]. Environ Sci Pollut Res Int, 2021, 28(10): 12960-12967.
[17]Xu QR, Tang J, Liao HY, et al. Long non-coding RNA MEG3 mediates the miR-149-3p/FOXP3 axis by reducing p53 ubiquitination to exert a suppressive effect on regulatory T cell differentiation and immune escape in esophageal cancer [J]. J Transl Med, 2021, 19(1): 264-278.
[18]Yan Y, Zheng L, Du Q, et al. Interferon regulatory factor 1(IRF-1) activates anti-tumor immunity via CXCL10/CXCR3 axis in hepatocellular carcinoma (HCC) [J]. Cancer Lett, 2021, 506(1): 95-106.
[19]Karin N. CXCR3 ligands in cancer and autoimmunity, chemoattraction of effector T cells, and beyond [J]. Front Immunol, 2020, 11(1): 976-985.
[20]Cai W, Shi L, Zhao J, et al. Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice [J]. J Clin Invest, 2022, 32(15): 1-17.

相似文献/References:

[1]郭　芳　张　铭　李中振　梁恩和.动脉瘤性蛛网膜下腔出血后分流依赖性脑积水的危险因素分析[J].中国临床神经外科杂志,2016,(02):73.[doi:10.13798/j.issn.1009-153X.2016.02.004]
　GUO Fang,ZHANG Ming,LI Zhong-zhen,et al.Risk factors related to shunt-dependent hydrocephalus in patients with aneurysmal subarachnoid hemorrhage[J].,2016,(01):73.[doi:10.13798/j.issn.1009-153X.2016.02.004]
[2]邹志鹏　杨　海　夏军勇　张化明　张　华　魏　恒　马江红.行血管内治疗的高龄颅内动脉瘤患者脑血管痉挛的临床分析[J].中国临床神经外科杂志,2015,(07):401.[doi:10.13798/j.issn.1009-153X.2015.07.006]
　ZOU Zhi-peng,YANG Hai,XIA Jun-yong,et al.Clinical analysis of symptomatic cerebral vasospasm after endovascular treatment of ruptured cerebral aneurysms in patients of 70 and over 70 years[J].,2015,(01):401.[doi:10.13798/j.issn.1009-153X.2015.07.006]
[3]郎胜坤　许厚银　董月青　马铁柱　孙　艳　郝芊芊　王慧敏.磁共振灌注成像评估蛛网膜下腔出血后脑血管痉挛的价值[J].中国临床神经外科杂志,2016,(09):517.[doi:10.13798/j.issn.1009-153X.2016.09.002]
　LANG Sheng-kuan,XU Hou-yin,DONG Yue-qing,et al.Evaluation of cerebral vasospasm after aneurysmal subarachnoid hemorrhage by magnetic resonance perfusion weighted imaging[J].,2016,(01):517.[doi:10.13798/j.issn.1009-153X.2016.09.002]
[4]王世龙,赵　冬,许　晖,等.血脂水平与动脉瘤性蛛网膜下腔出血后迟发性脑缺血的相关性[J].中国临床神经外科杂志,2016,(11):685.[doi:10.13798/j.issn.1009-153X.2016.11.011]
　WANG Shi-long,ZHAO Dong,XU Hui,et al.Relationship of serum lipid with delayed cerebral ischemia following the aneurysmal subarachnoid hemorrhage[J].,2016,(01):685.[doi:10.13798/j.issn.1009-153X.2016.11.011]
[5]潘逸珩　雷军荣　段　波　魏德胜　陈　亮　陈志明　秦　军.动脉瘤性蛛网膜下腔出血术后脑梗死的临床特点及其发生的危险因素分析[J].中国临床神经外科杂志,2017,(01):4.[doi:10.13798/j.issn.1009-153X.2017.01.002]
　PAN Yi-heng,LEI Jun-rong,DUAN Bo,et al.Analysis of clinical features of postoperative cerebral infarction and the factors related to it in patients with aneurysmal subarachnoid hemorrhage[J].,2017,(01):4.[doi:10.13798/j.issn.1009-153X.2017.01.002]
[6]谢　飞　陈志远　曾家良　叶勇强.DSA联合血清SICAM-1评估颅内破裂动脉瘤术后脑血管痉挛的效果[J].中国临床神经外科杂志,2017,(09):617.[doi:10.13798/j.issn.1009-153X.2017.09.003]
　XIE Fei CHEN Zhi-yuan ZENG Jia-liang,Ye Yong-qiang.Application of DSA combined with serum SICAM-1 to the evaluation of cerebral vasospasm after SAH due to aneurysmal rupture[J].,2017,(01):617.[doi:10.13798/j.issn.1009-153X.2017.09.003]
[7]阮　东　王　芳　郭丽蕊　彭红梅　陈谦学　刘宝辉.C-反应蛋白与动脉瘤性蛛网膜下腔出血临床预后的关系[J].中国临床神经外科杂志,2017,(09):626.[doi:10.13798/j.issn.1009-153X.2017.09.006]
　RUAN Dong,WANG Fang,GUO Li-rui,et al.Relationship between serum level of C-reactive protein and prognoses in patients with aneurysmal subarachnoid hemorrhage[J].,2017,(01):626.[doi:10.13798/j.issn.1009-153X.2017.09.006]
[8]阿库布千、李祥龙、罗　鑫、周　江、李　昊、刘　亮、 陈礼刚、夏祥国.亚洲人群动脉瘤性蛛网膜下腔出血高压氧辅助治疗疗效的Meta分析[J].中国临床神经外科杂志,2017,(11):757.[doi:10.13798/j.issn.1009-153X.2017.11.008]
　AKU Bu-qian,LI Xiang-long,LUO Xin,et al.Meta-analysis of effects of hyperbaric oxygen therapy on clinical outcomes in Asian patients with aneurysmal subarachnoid hemorrhage[J].,2017,(01):757.[doi:10.13798/j.issn.1009-153X.2017.11.008]
[9]张　兰　库洪彬　王　敏　周　燕.动脉瘤性蛛网膜下腔出血病人血清sLOX-1、netrin-1水平变化及其与病人预后的关系[J].中国临床神经外科杂志,2020,(06):377.[doi:10.13798/j.issn.1009-153X.2020.06.013]
　ZHANG Lan,KU Hong-bin,WANG Min,et al.Relationship between serum levels of sLOX-1 and netrin-1 and progosis of patients with aneurysmal subarachnoid hemorrhage[J].,2020,(01):377.[doi:10.13798/j.issn.1009-153X.2020.06.013]
[10]李兵兵　洪景芳　李克磊　张　灏　张尚明　王守森.动脉瘤性蛛网膜下腔出血对脑静脉循环的影响[J].中国临床神经外科杂志,2021,26(01):17.[doi:10.13798/j.issn.1009-153X.2021.01.006]
　LI Bing-bing,HONG Jing-fang,LI Ke-lei,et al.Effects of aneurysmal subarachnoid hemorrhage on cerebral venous circulation[J].,2021,26(01):17.[doi:10.13798/j.issn.1009-153X.2021.01.006]

备注/Memo

备注/Memo:
（2022-11-03收稿，2022-12-20修回）
更新日期/Last Update: 2022-02-28