[1]李国亮　邸　方　杨亚东.葛根素对大鼠颅脑损伤的保护作用[J].中国临床神经外科杂志,2016,(08):479-482.[doi:10.13798/j.issn.1009-153X.2016.08.012]
　LI Guo-liang,DI Fang,YANG Ya-dong..Neuroprotective effects of puerarin on cerebral tissues injured by trauma and its mechanism in rats[J].,2016,(08):479-482.[doi:10.13798/j.issn.1009-153X.2016.08.012]
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葛根素对大鼠颅脑损伤的保护作用()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2016年08期
页码:
479-482
栏目:
论著
出版日期:
2016-08-18

文章信息/Info

Title:
Neuroprotective effects of puerarin on cerebral tissues injured by trauma and its mechanism in rats
文章编号:
1009-153X（2016）08-0479-04
作者:
李国亮　邸　方　杨亚东
430040，武汉市东西湖区人民医院神经外科（李国亮、邸　方）；438000 湖北，黄冈市中心医院重症医学科（杨亚东）
通讯作者：杨亚东，E-mail：lglwaike@126.com
Author(s):
LI Guo-liang1;  DI Fang1;  YANG Ya-dong2.
1. Department of Neurosurgery, People's Hospital of Dongxihu District of Wuhan City, Wuhan 430040, China;
2. Intensive Care Unit, Central Hospital of Huanggang City, Huanggang 438000, China
关键词:
颅脑损伤; 氧化应激; 炎症反应; 细胞凋亡; 大鼠; 葛根素
Keywords:
Puerarin;  Traumatic brain injury;  Oxidation-stress action;  Inflammatory action
分类号:
R 651.1+5
DOI:
10.13798/j.issn.1009-153X.2016.08.012
文献标志码:
A
摘要:
目的 探讨葛根素对大鼠颅脑损伤（TBI）的保护作用及其机制。方法 将45只SD大鼠随机分为假手术组、模型组、低剂量葛根素组、中剂量葛根素组和高剂量葛根素组，每组9只。采用Feeney氏自由落体法制备TBI大鼠模型。低、中、高剂量葛根素组腹腔注射葛根素，剂量分别为10、25、50 mg/kg。造模后1、3、7 d采用改良神经功能缺损评分（mNSS）评价神经功能。造模后7 d，干湿法测定脑组织含水量；ELISA法检测脑组织丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、过氧化氢酶（CAT）、核因子κB （NF-κB）、细胞间黏附分子-1（ICAM-1）、白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、caspase-3水平；免疫印迹法检测Bax、Bcl-2的表达。结果 葛根素能显著降低TBI大鼠mNSS（P<0.05），显著减轻脑组织水肿（P<0.05），显著降低脑组织MDA、SOD、GSH、CAT、NF-κB、ICAM-1、IL-6、TNF-α、caspase-3水平（P<0.05），显著下调Bax表达而上调Bcl-2表达（P<0.05）。结论 葛根素可通过减轻颅脑水肿、抑制氧化应激及炎性反应以及调节Bax/Bcl-2表达从而发挥神经保护作用。
Abstract:
Objective To investigate the effect of puerarin on the cerebral tissues injured by trauma and its mechanism in rats. Methods Forty-five SD rats were randomly divided into 5 groups of 9 animals each, i.e. sham operation, brain injury and 10 mg/kg, 25 mg/kg and 50 mg/kg puerarin treatment groups. The animal models of traumatic brain injury were established by Feeney method. Puerarin dose corresponding to the treatment group was intraperitoneally injected once a day for 7 days in all the treatment groups. The neurological functions were evaluated by modified neurological severity scale (mNSS) 1, 3 and 7 days after the injury in all the groups. The water contents in the injured cerebral tissues were determined 7 days after the injury. The expression of malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), nuclear factor-κB (NF-κB), tumor necrosis factor (TNF-α), intercellular cell adhesion molecule-1, interleukin-6 and caspase-3 in the cerebral tissues were detected by ELISA and the expressions of Bax and Bcl-2 protein in the cerebral tissues were detected by Western blot 7 days after the injury. Results The mNSS scores 1, 3 and 7 days after the injury and water contents in the injured cerebral tissues 7 days after the injury were significantly lower in all the treatment groups than those in the brain injury group (P<0.05). The levels of MDA、CAT、SOD and GSH, NF-κB, TNF-α, ICAM-1, IL-6 and caspase-3 expressions were significantly lower 7 days after the injury in all the treatment group than those in the brain injury group (P<0.05). The level of Bax protein expression was significantly lower and level of Bcl-2 protein expression was significantly higher in the cerebral tissues in all those in the brain injury group (P<0.05). Conclusions It is suggested that puerarin protects the cerebral tissues injured by trauma probably through the relief of cerebral edema, inhibition of oxidation-stress action and inflammatory action and regulation of Bcl-2 and Bax expression in the cerebral tissues.

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更新日期/Last Update: 2016-08-19