[1]韩芸峰　王振宇　于　涛.大鼠脊髓损伤模型建立及神经源性肠功能障碍的初步评价[J].中国临床神经外科杂志,2015,(10):617-620.[doi:10.13798/j.issn.1009-153X.2015.10.013]
　HAN Yun-feng,WANG Zhen-yu,YU Tao..Establishment of model of spinal cord injury and evaluation of neurogenic bowel disfunction in rats[J].,2015,(10):617-620.[doi:10.13798/j.issn.1009-153X.2015.10.013]
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大鼠脊髓损伤模型建立及神经源性肠功能障碍的初步评价()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2015年10期
页码:
617-620
栏目:
论著
出版日期:
2015-10-30

文章信息/Info

Title:
Establishment of model of spinal cord injury and evaluation of neurogenic bowel disfunction in rats
文章编号:
1009-153X（2015）10-0617-04
作者:
韩芸峰　王振宇　于　涛
100191 北京，北京大学第三医院神经外科
通讯作者：王振宇，E-mail：wzyu502@hotmail.com
Author(s):
HAN Yun-feng;  WANG Zhen-yu;  YU Tao.
Department of Neurosurgery, The Third Hospital, Peking University, Beijing 100191, China
关键词:
脊髓损伤; 神经源性肠功能障碍; 大鼠
Keywords:
Spinal cord injury;  Neurogenic bowel dysfunction;  Rats
分类号:
R 641; R 744
DOI:
10.13798/j.issn.1009-153X.2015.10.013
文献标志码:
A
摘要:
目的 探讨建立大鼠脊髓损伤模型的方法，并初步评价神经源性肠功能障碍的发生、发展及变化规律。方法 56只大鼠随机分为脊髓损伤组（n=40）、假手术组（n=8）和正常组（n=8），其中脊髓损伤组再根据损伤后时间分为伤后1 d组、3 d组、7 d组、14 d组、28 d组等5个亚组，每亚组8只。假手术组和正常组合称为非脊髓损伤组。采用自制改良Allen脊髓撞击器造成脊髓不完全性损伤，假手术组只暴露硬膜囊。采用肠道推进试验、粪便粒数及粪便含水率测定来评价脊髓损伤后肠功能情况。结果 与非脊髓损伤组相比，脊髓损伤组结肠推进率明显降低（P<0.05）；而且，脊髓损伤后1 d组结肠推进率降低最明显（P<0.05）。与非脊髓损伤组相比，脊髓损伤组粪便粒数明显增多（P<0.05）；而且，脊髓损伤后1 d组粪便粒数最多（P<0.05）。与非脊髓损伤组相比，脊髓损伤组粪便含水率明显降低（P<0.05）；而且，脊髓损伤后1 d组粪便含水率降低最明显（P<0.05）。结论 采用自制改良Allen脊髓撞击器能成功复制大鼠不完全性脊髓损伤模型；大鼠脊髓损伤后出现神经源性肠功能障碍，损伤后急性期（伤后1 d）尤为显著，随后逐渐改善。
Abstract:
Objective To establish the model of spinal cord injury (SCI) and study the occurrence, development and variation of neurogenic bowel dysfunction (NBD) in rats. Methods Fifty-six rats were randomly divided into spinal cord injury group (SCI group), sham operation group and blank control group. The sham operation and blank control groups were called non-SCI group together. SCI was established by modified Allen method. The spinal dural sac was exposed to air in the sham operation group. Intestinal transit test, fecal pellet number, and fecal water content rate were used to assess the dynamic of intestines. Results The colonic transit rate was significantly lower in SCI group than those in non-SCI group (P<0.05). The colonic transit rate was significantly lower in SCI group 1 day after SCI than those 28 days after SCI in SCI group and in the sham operation and blank control groups (P<0.05). The fecal pellet number was more in SCI group than those in non-SCI group (P<0.05). The fecal pellet number was more 1 day after SCI than that 28 days after SCI (P<0.05). The water content rate was significantly lower in SCI group than that in the sham operation group (P<0.05). Conclusions It is suggested that NBD which is caused by SCI induced by the modified Allen method is more severe early after SCI than that late after SCI. The occurrence, development and variation of NBD after SCI may be evaluated by the intestinal transit test, fecal pellet number and fecal water content rate.

参考文献/References:

[1] Awad RA. Neurogenic bowel dysfunction in patients with spinal cord injury, myelomeningocele, multiple sclerosis and Parkinson's disease [J]. World J Gastroenterol, 2011, 17(46): 5035-5048.
[2] Ruiz-López MC, Coss-Adame E. Quality of life in patients with different constipation subtypes based on the Rome Ⅲ criteria [J]. Rev Gastroenterol Mex, 2015, 80(1): 13-20.
[3] 尚云龙，李一帆，宁云峰，等. 大鼠脊髓损伤模型的解剖学 参考定位[J]. 解剖学研究，2013，35（6）：412-414.
[4] Beattie MS, Bresnahan JC, Basso DM. Graded histological and locomotor outcomes after spinal cord contusion using the NYU weight-drop device versus transection [J]. Exp Neurol, 1996, 139(2): 244-256.
[5] 朱飞叶. 芍药甘草汤对慢传输型便秘大鼠的作用及其对 肠神经递质及SCF/c-kit信号途径的作用机制研究[D]. 浙江中医药大学，2014. 35-40.
[6] Liu CW, Huang CC, Yang YH, et al. Relationship between neurogenic bowel dysfunction and health-related quality of life in persons with spinal cord injury [J]. J Rehabil Med, 2009, 41(1): 35-40.
[7] Noonan VK, Kopec JA, Zhang H, et al. Impact of associated conditions resulting from spinal cord injury on health status and quality of life in people with traumatic central cord syndrome [J]. Arch Phys Med Rehabil, 2008, 89(6): 1074- 1082.
[8] 董贤慧，高维娟. 脊髓损伤动物模型建立方法研究进展 [J]. 神经解剖学杂志，2014，30（1）：117-120.
[9] 朱黎婷，朱　毅. 脊髓损伤神经源性肠道功能障碍的诊 断、评价和康复治疗现况[J]. 中国康复医学杂志，2013， 28（12）：1163-1167.
[10] Media S, Christensen P, Lauge I, et al. Reproducibility and validity of radiographically determined gastrointestinal and segmental colonic transit times in spinal cord-injured patients [J]. Spinal Cord, 2009, 47(1): 72-75.
[11] Liu GX, Yang YX, Yan J, et al. Glial-derived neurotrophic factor reduces inflammation and improves delayed colonic transit in rat models of dextran sulfate sodium-induced colitis [J]. Int Immunopharmacol, 2014, 19(1): 145-152.

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备注/Memo

备注/Memo:
基金项目：北京市自然科学基金（7144253）
更新日期/Last Update: 2015-10-30