[1]罗志华　张严国　黄从刚　王春燕　李乾锋.lncRNA MEG3表达水平及甲基化状态与垂体腺瘤术后复发的关系[J].中国临床神经外科杂志,2020,(12):825-827.[doi:10.13798/j.issn.1009-153X.2020.12.003]
　LUO Zhi-hua,ZHANG Yan-guo,HUANG Cong-gang,et al.Relationship between expression level and methylation status of lncRNA MEG3 and postoperative recurrence of pituitary adenoma[J].,2020,(12):825-827.[doi:10.13798/j.issn.1009-153X.2020.12.003]
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lncRNA MEG3表达水平及甲基化状态与垂体腺瘤术后复发的关系()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2020年12期
页码:
825-827
栏目:
论著
出版日期:
2020-12-25

文章信息/Info

Title:
Relationship between expression level and methylation status of lncRNA MEG3 and postoperative recurrence of pituitary adenoma
文章编号:
1009-153X（2020）12-0825-03
作者:
罗志华　张严国　黄从刚　王春燕　李乾锋
430010，武汉市第一医院神经外科（罗志华、张严国、黄从刚、王春燕、李乾锋）
Author(s):
LUO Zhi-hua;  ZHANG Yan-guo;  HUANG Cong-gang;  WANG Chun-yan;  LI Qian-feng.
Department of Neurosurgery, Wuhan No.1 Hospital, Wuhan 430010, China
关键词:
垂体腺瘤; 长链非编码核糖核酸; lncRNA MEG3; 启动子甲基化; 术后复发; 危险因素
Keywords:
Pituitary adenomas;  LncRNA MEG3;  Gene methylation;  Postoperative recurrence
分类号:
R 739.41; Q 786
DOI:
10.13798/j.issn.1009-153X.2020.12.003
文献标志码:
A
摘要:
目的 探讨长链非编码核糖核酸（lncRNA）MEG3表达水平及甲基化状态与垂体腺瘤术后复发的关系。方法 回顾性分析2015年1月~2017年12月手术治疗的75例垂体腺瘤的临床资料。另外取10例颅脑损伤内减压术中切除正常脑组织为对照组。采用qRT-PCR法和甲基化特异性聚合酶链反应检测垂体腺瘤组织lncRNA MEG3表达和甲基化状态。垂体腺瘤病人随访至2020年8月，主要研究终点为术后复发。结果 垂体腺瘤组织lncRNA MEG3相对表达量明显低于正常脑组织（P<0.05），同时其基因甲基化率明显高于正常脑组织（P<0.05）。随访期间，19例复发，复发率为25.33%。与未复发组相比，复发组lncRNA MEG3相对表达量明显降低（P<0.05），同时其基因启动子甲基化率明显升高（P<0.05）。多因素Cox比例回归风险模型分析结果显示，lncRNA MEG3相对表达水平降低、启动子甲基化率增高是垂体腺瘤术后复发的独立影响因素（P<0.05）。Kaplan-Meier生存曲线分析结果显示，lncRNA MEG3低表达组术后复发时间（中位数为17.0个月）较高表达组（中位数为24.0个月）明显缩短（P<0.001）；MEG3基因甲基化组术后复发时间（中位数为17.0个月）较非甲基化组（中位数为60.0个月）明显缩短（P<0.05）。结论 与正常脑组织相比，垂体腺瘤组织lncRNA MEG3呈低表达，其启动子甲基化率较高，与术后复发密切相关。
Abstract:
Objective To explore the relationship between the expression level and methylation status of lncRNA MEG3 and the postoperative recurrence of pituitary adenomas (PA). Methods The clinical data of 75 PA patients who underwent surgical resection in our hospital from January 2015 to December 2017 were retrospectively analyzed. The normal brain tissues obtained from 10 patients with traumatic brain injury underwent decompression were served as the control group. The expression level and methylation status of MEG3 were detected by qRT-PCR and MSP, respectively. PA patients were followed up until August 2020, and the main endpoint was postoperative recurrence. Results The relative expression level of MEG3 in PA tissues was significantly lower than that in normal pituitary tissues (P<0.05), while the methylation rate of MEG3 in PA tissues was sidnificantly higher than that in normal pituitary tissues (P<0.05). During the follow-up period, there were 19 PA patients recurred. Compared with PA tissues without recurrence, the relative expression levels of MEG3 were significantly lower and the methylation rate of MEG3 gene promoter was significantly higher in PA paients with recurrence (P<0.05). Multivariate Cox proportional regression risk model analysis showed that the high methylation rate of MEG3 gene (HR=11.835, P=0.002) and the low relative expression level of MEG3 (HR=0.160, P=0.015) were independent risk factors for postoperative recurrence of PA. The Kaplan-Meier survival curve analysis showed that the median postoperative recurrence time was significantly shorter with low MEG3 expression and high methylation than those whithout (P=0.010). Conclusion The expression level of lncRNA MEG3 is low and the methylation rate of MEG3 gene promoter is high in PA tissues, which is correlated to the postoperative recurrence of PA.

参考文献/References:

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备注/Memo

备注/Memo:
通讯作者：张严国，E-mail：linwei_buju_2003@163.com
更新日期/Last Update: 2020-12-25