[1]贾　杉　鲁德忠.TLR4基因突变对小鼠坐骨神经损伤修复的影响[J].中国临床神经外科杂志,2019,(12):755-757.[doi:10.13798/j.issn.1009-153X.2019.12.012]
　JIA Shan,LU De-zhong..Effect of inflammatory factors expression induced by Toll-like receptor 4 mutation on repair of injured sciatic nerve[J].,2019,(12):755-757.[doi:10.13798/j.issn.1009-153X.2019.12.012]
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TLR4基因突变对小鼠坐骨神经损伤修复的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2019年12期
页码:
755-757
栏目:
实验研究
出版日期:
2019-12-24

文章信息/Info

Title:
Effect of inflammatory factors expression induced by Toll-like receptor 4 mutation on repair of injured sciatic nerve
文章编号:
1009-153X（2019）12-0755-03
作者:
贾　杉　鲁德忠
710077 西安，陕西省中医医院神经外科（贾　杉）；832000 新疆，石河子市人民医院神经外科（鲁德忠）
Author(s):
JIA Shan1;  LU De-zhong2.
1. Department of Neurosurgery, Shanxi Provincial Hospital of Traditional Chinese Medicine, Xi'an 710077, China; 2. Department of Neurosurgery, Shihezi Municipal People's Hospital, Shihezi 832000, China
关键词:
坐骨神经损伤; Toll样受体4; C3H/HeJ小鼠; 炎症因子; 神经修复
Keywords:
Sciatic nerve injury;  Toll-like receptor 4;  C3H/HeJ;  Inflammatory factors;  Expressions;  Nerve repair;  Mice
分类号:
R 745.4+9; Q 786
DOI:
10.13798/j.issn.1009-153X.2019.12.012
文献标志码:
A
摘要:
目的 探讨Toll样受体4（TLR4）基因突变达对小鼠坐骨神经损伤修复的影响。方法 取10只C3H/HeJ小鼠（TLR4基因突变作为突变组，20只C3H/HeN小鼠（TLR4基因正常）随机分为假手术组（n=10）和模型组（n=10）。突变组和模型组在暴露的坐骨神经中部用止血钳夹持60 s以建立小鼠坐骨神经损伤模型，假手术组仅暴露坐骨神经而不进行夹伤。造模后4周，采用坐骨神经功能指数（SFI）评分评定坐骨神经功能，然后每组取3只小鼠行HE染色观察坐骨神经病理改变，每组取3只小鼠采用RT-PCR检测坐骨神经组织白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）mRNA表达水平，每组取4只小鼠采用免疫印迹法检测坐骨神经组织生长相关蛋白43（GAP43）、p75神经营养素受体（p75NTR）蛋白表达水平。结果 与假手术组相比，模型组SFI评分显著降低（P<0.05），HE染色显示细胞形态异常并出现大量嗜中性粒细胞和巨噬细胞，坐骨神经组织IL-1β、IL-6、TNF-α mRNA表达水平以及GAP43、p75NTR蛋白表达水平均显著增高（P<0.05）。与模型组相比，突变组SFI评分明显增高（P<0.05），组织结构病理改变明显改善，IL-1β、IL-6、TNF-α mRNA表达水平以及GAP43、p75NTR蛋白表达水平显著降低（P<0.05）。结论 TLR4基因突变可促进小鼠坐骨神经损伤后修复，可能与降低IL-1β、IL-6、TNF-α等炎症因子水平有关。
Abstract:
Objective To investigate the effect of low expression of inflammatory factors induced by toll-like receptor 4 (TLR4) mutation on the repair of injured sciatic nerve. Methods Ten C3H/HeJ mice were served as mutant injury group (n=10), and 20 C3H/HeN mice were randomly divided into sham operation group (n=10) and normal injury group (n=10). The models of sciatic nerve injury were made by clipping the middle part of sciatic nerve for 60 seconds in all the mice of mutant and normal injury groups. The sciatic nerve function was assessed by sciatic function index (SFI), the histopathological changes were observed, and the levels of interleukin-1β (IL-1β), interleukin-6 (IL) and tumor necrosis factor-α (TNF-α) mRNA expressions, and the levels of growth-associated protein-43 (GAP43) and p75 neurotrophin receptor (p75NTR) protein expressions in the sciatic nerve tissues were detected respectively by RT-PCR and western blot 4 weeks after the injury. Results The histopathological examination showed that the inflammatory reaction of sciatic nerve tissues was weaker in the mutant injury group than that in the normal injury group. The levels of IL-1β, IL-6 and TNF-α mRNA expressions in the sciatic nerve tissues and SFI were significantly lower in the sham group than those in the mutant injury group (P<0.05), which were significantly lower than those in the normal injury group (P<0.05).The levels of GAP43 and p75NTR protein expressions in the sciatic nerve tissues were significant higher in the sham group than those in the mutant injury group (P<0.05), which were significantly higher than those in the normal injury group (P<0.05). Conclusion It is suggested that TLR4 may accelerate the repair of the injured sciatic nerve injury by inducing the low expression of IL-1β, IL-6 and TNF-α and high expressions of GAP43 and p75NTR in the injured sciatic nerve tissues.

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备注/Memo

备注/Memo:
通讯作者：鲁德忠，E-mail：chenyanlin14@sina.com（2019-05-24收稿，2019-09-30修回）
更新日期/Last Update: 2019-12-23