[1]陈其钻 杨朋范 钟忠辉 林 巧 梅 珍 裴家生 王守森.蝎毒耐热蛋白对癫痫大鼠海马组织BDNF及NPY表达的影响[J].中国临床神经外科杂志,2020,(02):98-100.[doi:10.13798/j.issn.1009-153X.2020.02.012]
 CHEN Qi-zuan,YANG Peng-fan,ZHONG Zhong-hui,et al.Effects of scorpion venom heat-resistant protein on expressions of BDNF and NPY in hippocampal tissues in rats with epilepsy[J].,2020,(02):98-100.[doi:10.13798/j.issn.1009-153X.2020.02.012]
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蝎毒耐热蛋白对癫痫大鼠海马组织BDNF及NPY表达的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2020年02期
页码:
98-100
栏目:
实验研究
出版日期:
2020-02-25

文章信息/Info

Title:
Effects of scorpion venom heat-resistant protein on expressions of BDNF and NPY in hippocampal tissues in rats with epilepsy
文章编号:
1009-153X(2020)02-0098-03
作者:
陈其钻 杨朋范 钟忠辉 林 巧 梅 珍 裴家生 王守森
350025 福州,中国人民解放军联勤保障部队第900医院神经外科(陈其钻、杨朋范、钟忠辉、林 巧、梅、珍、裴家生、王守森)
Author(s):
CHEN Qi-zuan YANG Peng-fan ZHONG Zhong-hui LIN Qiao MEI Zhen PEI Jia-shen WANG Shou-sen.
Department of Neurosurgery, No. 900 Hospital, Logistic Support, Command, PLA, Fuzhou 350025, China
关键词:
癫痫蝎毒耐热蛋白FeCl2诱导癫痫模型大鼠
Keywords:
Scorpion venom heat-resistant protein FeCl2 Epilepsy Hippocampal neurons BDNF NPY Rats
分类号:
R 742.1; Q 786
DOI:
10.13798/j.issn.1009-153X.2020.02.012
文献标志码:
A
摘要:
目的探讨蝎毒耐热蛋白(SVHRP)对癫痫大鼠海马组织脑源性神经营养因子(BDNF)和神经肽Y(NPY)表达的影响。方法 健康成年雄性SD大鼠180只,按随机区组法随机分为3组(SVHRP组、模型组、正常组),每组60只动物。经颈部皮下注射FeCl2诱发癫痫急性发作,选取4~5级(Racine的癫痫分级标准)癫痫发作的大鼠。HE染色观察大鼠海马组织形态,免疫印迹法及荧光免疫组织化学染色检测大鼠海马组织BDNF和NPY的表达。结果 HE染色结果显示模型组大鼠海马组织形态结果破坏严重,而SVHRP组明显改善。模型组和SVHRP组大鼠海马神经BDNF及NPY表达水平均明显低于正常组(P<0.05),但SVHRP组BDNF及NPY 表达水平明显高于模型组(P<0.05)。结论 SVHRP能够减轻癫痫大鼠海马组织病理损伤程度,上调海马组织BDNF及NPY表达。
Abstract:
Objective To investigate the effect of scorpion venom heat-resistant protein (SVHRP) on the expressions of BDNF and NPY in hippocampal tissues in rats with epilepsy. Methods One hundred and eighty rats were randomly divided into 3 groups of 60 animals each, i.e. SVHRP treatment group, model group and normal group. The epileptic models were made by subcutaneous injection of FeCl2. The expressions of brain-derived neurotrophic factor (BDNF) and neuropeptide Y (NPY) in the hippocampal tissues was detected by western blot and fluorescence immunohistochemical technique in all the animals. Results The levels of expressions of BDNF and NPY in hippocampal tissues were significantly higher in the normal group than those in the SVHRP treatment group (P<0.05), which were significantly higher than those in the model group (P<0.05). Conclusions SVHRP can increase the expressions of BDNF and NPY expressions in the hippocampal tissues in the rats with epilepsy.

参考文献/References:

[1] 史 磊,张天锡,杜聪颖,等. 中药全蝎活性成分、药理作 用及临床应用研究进展[J]. 辽宁中医药大学学报,2015, 17(4):89-91.
[2] 马向梅,王均宁. 有毒中药全蝎增效减毒配伍方法探讨 [J]. 中华中医药杂志,2018,33(8):3659-3661.
[3] Lughetti L, Lucaccioni L, Fugetto F, et al. Brain-derived neurotrophic factor and epilepsy: a systematic review [J]. Neuropeptides, 2018, 72: 23-29.
[4] 卓俊哲,黄 婷,雷梓妍,等. 蝎毒对Hela细胞的体外药 理学研究[J]. 经济动物学报,2019,22(1):1-5.
[5] Wang XG, Zhu DD, li N, et al. Scorpion venom heat-resistant peptide is neuroprotective against cerebral ischemia- reperfusion injury in association with the NMDA-MAPK pathway [J]. Neurosci Bull, 2019, 9(9): 1673-7067.
[6] Wang T, Wang SW, Zhang Y, et al. Scorpion venom heat- resistant peptide (SVHRP) enhances neurogenesis and neu- rite outgrowth of immature neurons in adult mice by up- regulating brain-derived neurotrophic factor (BDNF) [J]. PLoS One, 2014, 9(10):84-94.
[7] Husum H, Bolwig T G, Sanchez C, et al. Levetiracetam prevents changes in levels of brain-derived neurotrophic factor and neuropeptide Y mRNA and of Y1- and Y5-like receptors in the hippocampus of rats undergoing amygdala kindling: implications for antiepileptogenic and mood- stabilizing properties [J]. Epilepsy Behav, 2004, 5(2): 204- 215.
[8] Poulsen FR, Jahnsen H , Blaabjerg M, et al. Pilocarpine- induced seizure- like activity with increased BNDF and neuropeptide Y expression in organotypic hippocampal slice cultures [J]. Brain Res, 2002, 950(1-2): 103-118.
[9] Powell KL, Fitzqerald X, Shallue C, et al. Gene therapy mediated seizure suppression in genetic generalised epile- psy: neuropeptide Y overexpression in a rat model [J]. Neurobiol Dis, 2018, 113: 23-32.
[10] Howell OW, Doyle K, Goodman JH, et al. Neuropeptide Y stimulates neuronal precursor proliferation in the post-natal and adult dentate gyrus [J]. J Neurochem, 2005, 93(3): 560- 570.

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备注/Memo

备注/Memo:
基金项目:全军后勤科研计划面上项目(CNJ14J008) 通讯作者:杨朋范,E-mail:neurosurg.yang@163.com(2019-07-09收稿,2019-12-09修回)
更新日期/Last Update: 2020-02-25