[1]廖小俊　李　兰　陈亚星　文泽贤.高压氧促进小鼠脊髓损伤后功能恢复[J].中国临床神经外科杂志,2021,26(01):35-38.[doi:10.13798/j.issn.1009-153X.2021.01.012]
　LIAO Xiao-jun,LI Lan,CHEN Ya-xin,et al.Hyperbaric oxygen therapy promotes functional rehabilitation of mice after spinal cord injury[J].,2021,26(01):35-38.[doi:10.13798/j.issn.1009-153X.2021.01.012]
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高压氧促进小鼠脊髓损伤后功能恢复()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
26
期数:
2021年01期
页码:
35-38
栏目:
实验研究
出版日期:
2021-01-25

文章信息/Info

Title:
Hyperbaric oxygen therapy promotes functional rehabilitation of mice after spinal cord injury
文章编号:
1009-153X（2021）01-0035-04
作者:
廖小俊　李　兰　陈亚星　文泽贤
810007 西宁，解放军联勤保障部队第941医院神经外科（廖小俊）；400038 重庆，陆军军医大学第一附属医院神经外科（李　兰、陈亚星、文泽贤）
Author(s):
LIAO Xiao-jun1;  LI Lan2;  CHEN Ya-xin2;  WEN Ze-xian2.
1. Department of Neurosurgery, The 941st Hospital of Joint Logistics Force, PLA, Xining 810007, China; 2. Department of Neurosurgery, The First Affiliated Hospital of Army Military Medical University, Chongqing 400038, China
关键词:
脊髓损伤; 高压氧治疗; Nrf2; 小鼠
Keywords:
Spinal cord injury;  Hyperbaric oxygen therapy;  Mouse;  Nrf2
分类号:
R 651.2; Q 786
DOI:
10.13798/j.issn.1009-153X.2021.01.012
文献标志码:
A
摘要:
目的 探讨高压氧（HBO）治疗小鼠脊髓损伤的效果及机制。方法 将雌性ICR小鼠45只随机分为3组：假手术组（n=15）、模型组（n=15）、HBO组（n=15）。应用血管夹损伤脊髓组织制作脊髓损伤模型。模型组造模后常规护理，HBO组行HBO治疗。造模后1、7、28 d采用BMS评分评估小鼠后肢运动功能；造模后7 d，采用免疫印迹法检测损伤脊髓组织Nrf2蛋白表达情况，采用免疫荧光染色观察细胞核Nrf2移位情况。结果 假手术组BMS评分均为9分。造模后1 d，模型组和HBO组BMS评分均为0分，后肢完全瘫痪。造模7 d，模型组BMS评分仍为0分；HBO组MBS评分明显高于模型组（P<0.05），但明显低于假手术组（P<0.05）；造模后28 d，模型组和HBO组BMS评分均明显提高（P<0.05），HBO组提高更明显（P<0.05）。造模后7 d，免疫荧光染色显示，假手术组没有发现Nrf2核移位，模型组可见少量Nrf2核移位，HBO组Nrf2核移位明显增多；免疫印迹法结果显示，模型组和HBO组Nrf2表达水平明显增高（P<0.05），HBO组增高更明显（P<0.05）。结论 HBO治疗可显著促进小鼠脊髓损伤后神经功能恢复，机制可能与促进Nrf2表达及核移位有关。
Abstract:
Objective To explore the effect of hyperbaric oxygen (HBO) therapy on the spinal cord injury (SCI) in mice. Methods Forty-five female ICR mice were randomly divided into 3 groups: sham operation group (n=15), injury group (n=15), and HBO group (n=15). The spinal cord tissue was injured by the vascular clip to make the SCI model. The injury group received routine care after injury, and the HBO group received HBO therapy. BMS score was used to evaluate the motor function of mice hind limbs 1, 7, and 28 days after injury. Seven day after injury, the expression of Nrf2 protein in the injured spinal cord tissues was detected by Western blotting, and the shift of nuclear Nrf2 was observed by immunofluorescence staining. Results Seven days after injury, the MBS score of the HBO group was significantly higher than that of the injury group (P<0.05), but it was significantly lower than that of the sham operation group (P<0.05). Twenty-eight days after injury, the BMS scores in the injury and HBO groups significantly increased (P<0.05), and the BMS score in the HBO group was significantly higher than that in the injury group (P<0.05). Seven days after injury, immunofluorescence staining showed that there was no nuclear translocation of Nrf2 in the sham operation group, a small amount of nuclear translocation of Nrf2 in the injury group, and significant increase in the nuclear translocation of Nrf2 in the HBO group. The expression level of Nrf2 was significantly higher in the HBO group than the injury group (P<0.05), which was significantly higher than the sham operation group (P<0.05). Conclusion HBO therapy can significantly promote the recovery of nerve function after SCI in mice, which may be related to the promotion of Nrf2 expression and nuclear translocation.

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备注/Memo

备注/Memo:
2020-02-03收稿
更新日期/Last Update: 2021-01-25