[1]麦麦提依明·托合提,张诚,黄啸元,等.上调miR-124-3p表达增加U-87 MG人胶质母细胞瘤细胞的放疗敏感性[J].中国临床神经外科杂志,2024,29(07):422-426.[doi:10.13798/j.issn.1009-153X.2024.07.008]
　MAIMAITIYIMING Tuoheti,ZHANG Cheng,HUANG Xiao-yuan,et al.Upregulation of miR-124-3p expression increases the radiosensitivity of U-87 MG human glioblastoma cells[J].,2024,29(07):422-426.[doi:10.13798/j.issn.1009-153X.2024.07.008]
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上调miR-124-3p表达增加U-87 MG人胶质母细胞瘤细胞的放疗敏感性()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
29
期数:
2024年07期
页码:
422-426
栏目:
实验研究
出版日期:
2024-07-30

文章信息/Info

Title:
Upregulation of miR-124-3p expression increases the radiosensitivity of U-87 MG human glioblastoma cells
文章编号:
1009-153X（2024）07-0422-05
作者:
麦麦提依明·托合提; 张诚; 黄啸元; 高峰; 帕热哈提江·依孜木; 阿卜杜喀迪尔·牙森; 董军; 杨小朋; 吴永刚
830001乌鲁木齐，新疆维吾尔自治区人民医院神经外科（麦麦提依明·托合提、张诚、黄啸元、高峰、帕热哈提江·依孜木、阿卜杜喀迪尔·牙森、董军、杨小朋、吴永刚）
Author(s):
MAIMAITIYIMING Tuoheti;  ZHANG Cheng;  HUANG Xiao-yuan;  GAO Feng;  PAERHATIJIANG Yizimu;  ABUDUKADIER Yasen;  DONG Jun;  YANG Xiao-peng;  WU Yong-gang
Department of Neurosurgery, The People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
关键词:
胶质母细胞瘤; U87 MG细胞; miR-124-3p; 细胞增殖; 放疗敏感性
Keywords:
Glioblastoma;  U87 MG cells;  miR-124-3p;  Cell proliferation;  Radiosensitivity
分类号:
R 739.41
DOI:
10.13798/j.issn.1009-153X.2024.07.008
文献标志码:
A
摘要:
目的 探讨miR-124-3p表达水平对U-87 MG人胶质母细胞瘤细胞（U87细胞）放疗敏感性的影响。方法 体外培养U87细胞，利用脂质体载体转染技术转染hsa-miR-124-3p mimics或hsa-miR-124-3p inhibitor质粒调控miR-124-3p表达；转染24 h后，取生长状态良好的U87细胞，在室温下用西门子Primus-M型直线加速器进行照射（16 Gy）模拟放疗；实时荧光定量PCR检测细胞miR-124-3p表达水平，CCK-8法检测细胞增殖能力。结果 转染hsa-miR-124-3p mimics质粒后，细胞miR-124-3p表达水平明显上调（P<0.05）；转染hsa-miR-124-3p inhibitor质粒后，细胞miR-124-3p表达水平明显下调（P<0.05）。上调miR-124-3p表达，U87细胞增殖活性明显降低（P<0.05）；下调miR-124-3p表达，U-87细胞增殖活性明显增高（P<0.05）。上调miR-124-3p表达联合放疗，U87细胞增殖活性进一步明显降低（P<0.05）；下调miR-124-3p表达联合放疗，U87细胞增殖活性明显降低，但仍明显高于未给予任何处理的U87细胞的增殖活性（P<0.05）。结论 上调miR-124-3p表达明显抑制U87细胞的增殖活性，并且显著增加U87细胞的放疗敏感性。
Abstract:
Objective To explore the effect of miR-124-3p expression on the radiosensitivity of U-87 MG human glioblastoma cells (U87 cells). Methods U87 cells were cultured in vitro. The expression of miR-124-3p was regulated by transfecting hsa-miR-124-3p mimics or hsa-miR-124-3p inhibitor plasmids using liposome vector transfection technology. After 24 hours of transfection, U87 cells in good growth condition were irradiated at room temperature using a Siemens Primus-M linear accelerator (16 Gy) to simulate radiotherapy. The expression level of miR-124-3p in U87 cells was detected by real-time fluorescence quantitative PCR, and the proliferation ability of U87 cells was detected by the CCK-8 method. Results After transfection with hsa-miR-124-3p mimics plasmids, the expression level of miR-124-3p in U87 cells was significantly upregulated (P<0.05); after transfection with hsa-miR-124-3p inhibitor plasmids, the expression level of miR-124-3p in U87 cells was significantly downregulated (P<0.05). Upregulating the expression of miR-124-3p significantly reduced the proliferation activity of U87 cells (P<0.05); downregulating the expression of miR-124-3p significantly increased the proliferation activity of U87 cells (P<0.05). The combination of upregulating miR-124-3p expression and radiotherapy further significantly reduced the proliferation activity of U87 cells (P<0.05); the combination of downregulating miR-124-3p expression and radiotherapy significantly reduced the proliferation activity of U87 cells, but was still significantly higher than the proliferation activity of U87 cells without any treatment (P<0.05). Conclusions Upregulating the expression of miR-124-3p significantly inhibits the proliferation activity of U87 cells and significantly increases their radiosensitivity.

参考文献/References:

[1]YIN Y, QIU S, PENG Y. Functional roles of enhancer of zeste homolog 2 in gliomas[J]. Gene, 2016, 576(1 Pt 2): 189-194.
[2]MERCATELLI N, GALARDI S, CIAFRE SA. MicroRNAs as multi-faceted players in glioblastoma multiforme[J]. Int Rev Cell Mol Biol, 2017, 333: 269-323.
[3]WU HM, WANG HD, TANG Y, et al. Differential expression of microRNAs in postoperative radiotherapy sensitive and resistant patients with glioblastoma multiforme[J]. Tumour Biol, 2015, 36(6): 4723-4730.
[4]NIEDER C, ANDRATSCHKE NH, GROSU AL. Re-irradiation for recurrent primary brain tumors[J]. Anticancer Res, 2016, 36(10): 4985-4895.
[5]PAW I, CARPENTER RC, WATABE K, et al. Mechanisms regulating glioma invasion[J]. Cancer Lett, 2015, 362(1): 1-7.
[6]NIYAZI M, ZEHENTMAYR F, NIEMOLLER OM, et al. MiRNA expression patterns predict survival in glioblastoma[J]. Radiat Oncol, 2011, 6: 153.
[7]BESSW A, SANTA J, FADRUS P, et al. MicroRNAs involved in chemo- and radioresistance of high-grade gliomas[J]. Tumour Biol, 2013, 34(4): 1969-1978.
[8]GU H, FENG DF. Bioinformatics analysis of peripheral blood miRNA in patients with severe traumatic brain injury[J]. Chin J Clin Neurosurg, 2019, 24(12): 749-751. 顾　浩，冯东福. 重型颅脑损伤病人外周血miRNA生物信息学分析[J]. 中国临床神经外科杂志，2019，24（12）：749-751.
[9]PENG ZS, TIAN DF, ZHANG SQ, et al. The effect of miR-370-3p on the proliferation ability of glioblastoma U87-MG cell line[J]. Chin J Clin Neurosurg, 2016, 21(4): 223-226. 彭泽生，田道锋，张申起，等. miR-370-3p对胶质母细胞瘤U87-MG细胞株增殖能力的影响[J]. 中国临床神经外科杂志，2016，21（4）：223-226.
[10]XU S, ZHAO N, HHUI L, et al. MicroRNA-124-3p inhibits the growth and metastasis of nasopharyngeal carcinoma cells by targeting STAT3[J]. Oncol Rep, 2016, 35(3): 1385-1394.
[11]DENG D, WANG L, CHEN Y, et al. MicroRNA-124-3p regulates cell proliferation, invasion, apoptosis, and bioenergetics by targeting PIM1 in astrocytoma[J]. Cancer Sci, 2016, 107(7): 899-907.
[12]EGUIA-AGUILAR P, PEREZPENA-DIAZCONTI M, BENADON-DARSZON E, et al. Reductions in the expression of miR-124-3p, miR-128-1, and miR-221-3p in pediatric astrocytomas are related to high-grade supratentorial, and recurrent tumors in Mexican children[J]. Childs Nerv Syst, 2014, 30(7): 1173-1181.
[13]YUAN Q, SUN T, YE F, et al. MicroRNA-124-3p affects proliferation, migration and apoptosis of bladder cancer cells through targeting AURKA[J]. Cancer Biomark, 2017, 19(1): 93-101.
[14]LEUNG LY, CHAN CP, LEUNG YK, et al. Comparison of miR-124-3p and miR-16 for early diagnosis of hemorrhagic and ischemic stroke[J]. Clin Chim Acta, 2014, 433: 139-144.
[15]ZHAO WH, WU SQ, ZHANG YD. Downregulation of miR-124 promotes the growth and invasiveness of glioblastoma cells involving upregulation of PPP1R13L[J]. Int J Mol Med, 2013, 32(1): 101-107.
[16]SILBER J, HASHIZUME R, FELIX T, et al. Expression of miR-124 inhibits growth of medulloblastoma cells[J]. Neuro-Oncol, 2013, 15(1): 83-90.
[17]SHEN K, ZHANG CY, YIN XQ, et al. Study on the mechanism of miR-124-3p and autophagy in LPS induced microglial cell death [J]J Nantong Univ, 2019, 39(2): 89-92. 沈　凯，张春燕，殷晓芹，等. miR-124-3p和自噬在LPS致小胶质细胞死亡中的作用机制研究[J]. 南通大学学报（医学版），2019，39（2）：89-92.
[18]DENG X, MA L, WU M, et al. miR-124 radiosensitizes human glioma cells by targeting CDK4[J]. J Neuro-Oncol, 2013, 114(3): 263-274.
[19]BAHREYNI-TOOSI MT, DOLAT E, KHANBABAEI H, et al. microRNAs: potential glioblastoma radiosensitizer by targeting radiation-related molecular pathways[J]. Mutat Res, 2019, 816-818: 111679.

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备注/Memo

备注/Memo:
（2022-04-04收稿，2024-05-09修回）
基金项目：新疆维吾尔自治区自然科学基金（2022D01C625，2019D01C107，2017D01C146）
通信作者：吴永刚，Email: wyg931543@163.com
更新日期/Last Update: 2024-07-30