[1]原高明　孟晓峰　郭孝龙　崔　涛　程小兵　郝晓伟　李泓江　史保中.兔细菌性脑室炎模型的建立[J].中国临床神经外科杂志,2018,(03):173-175181.[doi:10.13798/j.issn.1009-153X.2018.03.011]
　YUAN Gao-ming,MENG Xiao-feng,GUO Xiao-long,et al.Establishment of bacterial ventriculitis model in rabbits[J].,2018,(03):173-175181.[doi:10.13798/j.issn.1009-153X.2018.03.011]
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兔细菌性脑室炎模型的建立()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2018年03期
页码:
173-175181
栏目:
论著
出版日期:
2018-04-01

文章信息/Info

Title:
Establishment of bacterial ventriculitis model in rabbits
文章编号:
1009-153X（2018）03-0173-03
作者:
原高明　孟晓峰　郭孝龙　崔　涛　程小兵　郝晓伟　李泓江　史保中
作者单位：471003 洛阳，河南科技大学第一附属医院神经外科（原高明、孟晓峰、郭孝龙、崔　涛、程小兵、郝晓伟、史保中）；200011 上海，上海交通大学医学院附属第九人民医院神经外科（李泓江）
Author(s):
YUAN Gao-ming1;  MENG Xiao-feng2;  GUO Xiao-long1;  CUI Tao1;  CHENG Xiao-bing1;  HAO Xiao-wei1;  LI Hong Jiang2;  SHI Bao-zhong1.
1. Department of Neurosurgery, The First Affiliated Hospital, He'nan University of Sciences and Technology, Luoyang 471003, China; 2. Department of Neurosurgery, Shanghai Ninth People's Hospital, Medical School, Shanghai Jiaotong University, Shanghai 2000
关键词:
细菌性脑室炎; 模型; 表皮葡萄球菌; 兔
Keywords:
Bacterial ventriculitis;  Staphylococcus epidermidis;  Model;  Rabbit
分类号:
R 742.6; R 332
DOI:
10.13798/j.issn.1009-153X.2018.03.011
文献标志码:
A
摘要:
目的 探讨兔细菌性脑室炎模型的制作方法。方法 将成年新西兰大白兔36只随机分为模型组、对照组和正常组，每组12只。模型组侧脑室注入浓度为1×106 CFU/ml的表皮葡萄球菌混悬液0.5 ml；对照组侧脑室内注入0.5 ml生理盐水。结果 与造模前相比，模型组造模后肛温、血白细胞总数和中性粒细胞百分比以及脑脊液蛋白含量、细胞总数、白细胞数均明显升高（P<0.05），而脑脊液糖含量、氯化物含量均明显降低（P<0.05）。对照组及正常组肛温，血、脑脊液指标无明显改变。模型组血细菌培养阴性，脑脊液细菌培养阳性；对照组及正常组血、脑脊液细菌培养阴性。病理观察示模型组室管膜下大量中性粒细胞浸润，对照组所见正常。结论 接种0.5 ml浓度为1×106 CFU/ml的表皮葡萄球菌混悬液可成功建立兔细菌性脑室炎。
Abstract:
Objective To establish a model of bacterial ventriculitis in rabbit. Methods Thirty-six adult healthy New Zealand white rabbits were randomly divided into three groups of 12 animals each, i.e. model group, in which 0.5ml staphylococcus epidermidis suspension with 1×106CFU/ml was injected into the lateral ventricle of each rabbit, operation control group, in which 0.5ml 0.9% sodium chloride was injected into the lateral ventricle of each animal and normal control group, in which nothing was injected. The rectal temperature was monitored and total leukocyte count and neutrophils percentages of blood and the contents of glucose, protein and chloride of CSF were detected 6 and 12 hours after the injection. The blood and CSF were taken in order to culture bacteria before the animal sacrifice. All the animals were killed 12 hours after the injection and brain tissue was histopathologically observed. Results The rectal temperature, total leukocyte count and neutrophils percentage of blood and protein content, total number of cells and leukocyte count of CSF were significantly higher and glucose level and chloride level of CSF were significantly lower in the model group than those in the operation control and normal control groups (P<0.05). There were insignificant difference in the above-mentioned indexes between the operation control and normal control groups (P>0.05). The blood bacterial culture and CSF bacterial culture were negative and positive respectively in the model group and all negative in the operation control and normal control groups. The pathological observation showed that neutrophile granulocytes infiltrated into the ependymal tissues in the model group and the cerebral tissues were normal in the operation control and normal control groups. Conclusion The bacterial ventriculitis model can successfully be established by the injection of 0.5ml staphylococcus epidermidis suspension with 1×106CFU/ml into ventricles in the rabbits.

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备注/Memo

备注/Memo:
通讯作者：史保中，E-mail：sbzmd20569@163.com
更新日期/Last Update: 2018-05-05