[1]顾　浩、冯东福.重型颅脑损伤病人外周血miRNA生物信息学分析[J].中国临床神经外科杂志,2019,(12):749-751.[doi:10.13798/j.issn.1009-153X.2019.12.010]
　GU Hao,FENG Dong-fu..Bioinformatics study of microRNA in peripheral blood in patients with severe traumatic brain injury[J].,2019,(12):749-751.[doi:10.13798/j.issn.1009-153X.2019.12.010]
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重型颅脑损伤病人外周血miRNA生物信息学分析()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2019年12期
页码:
749-751
栏目:
论著
出版日期:
2019-12-24

文章信息/Info

Title:
Bioinformatics study of microRNA in peripheral blood in patients with severe traumatic brain injury
文章编号:
1009-153X（2019）12-0749-03
作者:
顾　浩、冯东福
201900上海，上海交通大学医学院附属第九人民医院神经外科（顾　浩、冯东福）；201900上海，上海交通大学医学院创伤医学研究所（冯东福）
Author(s):
GU Hao1;  FENG Dong-fu1; 2.
1. Department of Neurosurgery, Shanghai Ninth People's Hospital, Medical School, Shanghai Jiao Tong University, Shanghai 200025, China; 2. Institute of Traumatic Medicine, Medical School, Shanghai Jiao Tong University, Shanghai 200025, China
关键词:
重型颅脑损伤; 微小RNA; 外周血; 生物信息学
Keywords:
Severe traumatic brain injury;  MicroRNA;  Bioinformatics;  Analysis
分类号:
R 651.1+5; Q 786
DOI:
10.13798/j.issn.1009-153X.2019.12.010
文献标志码:
A
摘要:
目的 通过生物信息学方法分析重型颅脑损伤（sTBI）病人外周血微小RNA（miRNA）的靶基因及功能。方法 从GEO数据库中检索获取sTBI病人和对照组外周血的基因芯片数据，应用生物信息学方法筛选差异表达的miRNA，并进行靶基因预测和生物学功能及信号通路分析，构建miRNA及靶基因的调控网络。结果 检索得到芯片GSE21854，筛选得到145个差异表达的miRNA，预测得到靶基因共580个。这些靶基因的功能主要为细胞增殖负性调控、转换生长因子β受体信号通路负性调控等，主要分布在Ras信号通路、转换生长因子β信号通路等。miRNA及靶基因的调控网络图显示hsa-miR-125a-5p、hsa-miR-760、hsa-miR-217、hsa-miR-199a-3p、hsa-miR-543是调控核心。结论 sTBI病人外周血存在差异性表达的miRNA，hsa-miR-125a-5p、hsa-miR-760、hsa-miR-217、hsa-miR-199a-3p、hsa-miR-543与sTBI的进展密切相关。
Abstract:
Objective To study the target genes and their functions of microRNA (miRNA ) in the peripheral blood in the patients with severe traumatic brain injury (sTBI) by bioinformatics analysis. Methods The microarray data of the patients with sTBI were retrieved from GEO database, and the miRNAs with differential expression were screened by bioinformatics tools. Then the target genes of miRNAs with differential expression were analyzed. Gene ontology and signal path way analysis were performed in the target genes．miRNAs and their targeted genes networks were established. Results The data were obtained from GSE21854 and 145 differential expressed miRNAs were screened out. A total of 580 target genes of screened miRNA were obtained. The functions of these target genes mainly included negative regulation of cell proliferation, negative regulation of transforming growth factor β receptor signaling pathway and so on. The miRNA and their targeted genes networks analysis revealed that hsa-miR-125a-5p, hsa-miR-760, hsa-miR-217, hsa-miR-199a-3p and hsa-miR-543 were the key genes. Conclusions The differential expressed miRNAs exist in the peripheral blood in the patients with sTBI and hsa-miR-125a-5p, hsa-miR-760, hsa-miR-217, hsa-miR-199a-3p and hsa-miR-543 are closely associated with the progression of sTBI．

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金（81772059） 通讯作者：冯东福，E-mail：drneuro@163.com （2019-04-27收稿，2019-07-01修回 ）
更新日期/Last Update: 2019-12-23