[1]兰展仇洪冯磊黄章峰禤义张传东盘丽华.人脑胶质母细胞瘤miR-4516表达与病人预后的关系[J].中国临床神经外科杂志,2022,27(02):107-109.[doi:10.13798/j.issn.1009-153X.2022.02.012]
　LAN Zhan,QIU Hong,FENG Lei,et al.Relationship between expression of miR-4516 in human glioblastoma tissues and patients’ prognoses[J].,2022,27(02):107-109.[doi:10.13798/j.issn.1009-153X.2022.02.012]
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人脑胶质母细胞瘤miR-4516表达与病人预后的关系()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
27
期数:
2022年02期
页码:
107-109
栏目:
论著
出版日期:
2022-02-25

文章信息/Info

Title:
Relationship between expression of miR-4516 in human glioblastoma tissues and patients’ prognoses
文章编号:
1009-153X（2022）02-0107-03
作者:
兰展仇洪冯磊黄章峰禤义张传东盘丽华
547000广西河池，右江民族医学院附属河池医院（河池市人民医院）神经外科（兰展、仇洪、冯磊、黄章峰、禤义、张传东、盘丽华）
Author(s):
LAN Zhan;  QIU Hong;  FENG Lei;  HUANG Zhang-feng;  FU Yi;  ZHANG Chuan-dong;  PAN Li-hua.
Department of Neurosurgery, Hechi Hospital Affiliated to Youjiang Medical College for Nationalities (Hechi People’s Hospital), Hechi 547000, China
关键词:
胶质母细胞瘤; 微小核糖核酸; miR-4516; 预后
Keywords:
Glioblastoma;  MicroRNA;  MiR-4516;  Prognosis
分类号:
R 739.41; Q 786
DOI:
10.13798/j.issn.1009-153X.2022.02.012
文献标志码:
A
摘要:
目的 探讨人胶质母细胞瘤（GBM）组织微小核糖核酸-4516（miR-4516）的表达情况及其与病人预后的关系。方法选取 2015年 1月~2019年 5月手术切除的 GBM组织 89例和颅脑损伤内减压术切除的非肿瘤脑组织 35例为对照组，采用 qRT-PCR检测 miR-4516的表达水平，根据 miR-4516表达水平的中位数分为高表达和低表达。GBM病人术后随访 2年，记录生存情况。结果 GBM组织 miR-4516表达水平（[ 5.32±1.75）]明显高于对照组（[ 1.13±0.45）；P<0.01]。术后 2年随访，42例（47.19%）生存，47例 47（52.81%）死亡。多因素 Cox回归分析显示，miR-4516高表达是 GBM生存预后病例的独立危险因素（P<0.05）。生存曲线分析显示 miR-4516高表达组 2年累积生存率（27.45%）明显低于低表达组（73.68%；P<0.001）。结论 GBM组织 miR-4516表达增高，其高表达是病人预后不良的危险因素。
Abstract:
Objective To investigate the expression level of microRNA-4516 (miR-4516) in human glioblastoma (GBM) tissuesand its relationship with the patients prognoses. Methods The expression levels of miR-4516 were detected in GBM tissues obtainedfrom 89 GBM patients who underwent microsurgery and in non-tumor cerebral tissues obtained from 35 patients with traumatic braininjury (control group) who underwent decompression from January 2015 to may 2019, using qRT-PCR. The GBM patients were divideinto high-expression and low-expression groups according the medium expression level of miR-4516. The GBM patients were followedup for 2 years and the survval status was recorded. Results The expression level of miR-4516 in GBM tissues was significantly higherthan that in the control group (P<0.05). Of 89 GBM patients, 42 patients (47.19%) survived and 47 (52.81%) died at the end of thefollow-up. Multivariate Cox regression analysis showed that high expression of miR-4516 was an independent risk factor for the poorsurvival of GBM patients (P<0.05). Survival curve analysis showed that the 2-year cumulative survival rate (27.45%) of the miR-4516high expression group was significantly lower than that (73.68%) of the low expression group (P<0.001). Conclusions The expression of miR-4516 in GBM tissues is increased, and its high expression is a risk factor for poor prognosis of GBM patients.

参考文献/References:

[1] 代从新，王裕，马文斌 .胶质母细胞瘤综合治疗后假性进展[J].中华医学杂志， 2019，99（13）：1038-1040.
[2] 曹冰清，王涛，康涛，等.胶质母细胞瘤相关基因的筛选和生物信息学分析 [J].医学分子生物学杂志， 2018，15（4）：192-198.
[3] 徐阳，袁凡恩，孙前，等. miRNA在胶质母细胞瘤血管生成和侵袭的作用 [J].中国微侵袭神经外科杂志， 2019，24（7）：334-336.
[4] 王明明，唐瑞天，刘建雄 .胞外囊泡源性 miRNA在胶质母细胞瘤中作用的研究进展 [J].中国临床神经外科杂志， 2021，26（1）：50-52.
[5] 胡克琦，周达全，陈锋，等. miR-153下调 FOXR2、CDK8和 CDK13的表达抑制胶质母细胞瘤细胞增殖 [J].中国临床神经外科杂志， 2020，25（11）：767-770.
[6] 王艳新，叶富跃，王艳. miR-126表达水平及其基因启动子甲基化状态与胶质母细胞瘤术后复发的关系 [J].中国临床神经外科杂志， 2021，26（9）：682-686.
[7] 王福花，李峰，王春艳，等.血清 miRNA表达谱在肝细胞癌中的差异性研究 [J].中国中西医结合消化杂志， 2020，28（6）：25-28.
[8] Liu JB, Chen D, Liu HX, et al. A microRNA-4516 inhibitor sensitizes chemo-resistant gastric cancer cells to chemo-therapy by upregulating ING4 [J]. RSC Adv, 2018, 8(66):37795-37803.
[9] 任铭新，王可心，方一帆，等.胶质母细胞瘤的分子生物学研究进展[J].肿瘤学杂志， 2017，23（10）：863-867.
[10]刘森，沈野，于洪飞，等.基于 RNA-seq研究胶质母细胞瘤中差异表达基因及蛋白相互作用网络 [J].基因组学与应用生物学， 2017，36（4）：1280-1287.
[11]王睿健，张文超，尹涛. CREPT在人脑胶质母细胞瘤组织中的表达及其与病人预后的关系 [J].中国临床神经外科杂志， 2021，26（1）：20-22.
[12]杨雅婷，李晖. AEBP1在胶质母细胞瘤中的表达及临床意义 [J].中国临床神经外科杂志， 2020，25（8）： 521-524.
[13]刘志峰，陈永汉，姜浩. AMOTL2与 FKBP51表达水平与胶质母细胞瘤病人预后的关系 [J].中国临床神经外科杂志， 2021，26（10）：780-783.
[14]丁元杰，马箐，郭旭东，等.食管鳞癌患者血清差异表达 miRNAs的筛选[J].山东医药， 2018，58（15）：1-4.
[15] Ji EK, Kim BG, Jang Y, et al. The stromal loss of miR-4516 promotes the FOSL1-dependent proliferation and malig-nancy of triple negative breast cancer [J]. Cancer Lett, 2019,469(Pt 5): 256-265.
[16] Cui T, Gray A, Liu Z, et al. Abstract 5726:A novel tumor-promoting role for miR-4516 in glioblastoma [J]. CancerRes, 2017, 77(13 Suppl): 5726-5726.
[17] Jin XH, Lu S, Wang AF. Expression and clinical signifi-cance of miR-4516 and miR-21-5p in serum of patientswith colorectal cancer [J]. BMC Cancer, 2020, 20(1): 241.
[18] Chen S, Xu M, Zhao J, et al. MicroRNA-4516 suppressespancreatic cancer development via negatively regulatingorthodenticle homeobox 1 [J]. Int J Biol Sci, 2020, 16(12):2159-2169.
[19] Cui T, Bell EH, McElroy J, et al. miR-4516 is a novel prog-nostic biomarker and promotes tumorigenesis via targetingPTPN14-mediated regulation of the Hippo pathway inglioblastoma [J]. Int J Radiat Oncol, 2018, 102(3): e176-e177.
[20] Sebastian E, Cui T, Bell EH, et al. Characterization of a novel mir-4516-PTPN14 therapeutic resistance pathwayinduced by radiation treatment in glioblastoma [J]. Int JRadiat Oncol, 2020, 108(3): e572.

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更新日期/Last Update: 1900-01-01