[1]胡克琦 周达全 陈 锋 周 毅 敖祥生.miR-153下调FOXR2、CDK8和CDK13的表达抑制胶质母细胞瘤细胞增殖[J].中国临床神经外科杂志,2020,(11):767-770.[doi:doi:10.13798/j.issn.1009-153X.2020.11.011]
 HU Ke-qi,ZHOU Da-quan,CHEN Feng,et al.miR-153 inhibits proliferation of glioblastoma multiforme cell lines by down-regulation of FOXR2, CDK8 and CDK13[J].,2020,(11):767-770.[doi:doi:10.13798/j.issn.1009-153X.2020.11.011]
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miR-153下调FOXR2、CDK8和CDK13的表达抑制胶质母细胞瘤细胞增殖()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2020年11期
页码:
767-770
栏目:
实验研究
出版日期:
2020-11-25

文章信息/Info

Title:
miR-153 inhibits proliferation of glioblastoma multiforme cell lines by down-regulation of FOXR2, CDK8 and CDK13
文章编号:
1009-153X(2020)11-0767-04
作者:
胡克琦 周达全 陈 锋 周 毅 敖祥生
441021 湖北,襄阳市中心医院神经外科(胡克琦、周达全、陈 锋、周 毅、敖祥生)
Author(s):
HU Ke-qi ZHOU Da-quan CHEN Feng ZHOU Yi AO Xiang-sheng.
Department of Neurosurgery, Xiangyang Center Hospital, Xiangyang 441021, China
关键词:
胶质母细胞瘤微小核糖核酸miR-153细胞增殖
Keywords:
Glioblastoma multiforme miR-153 Cell proliferation
分类号:
R 739.41; Q 786
DOI:
doi:10.13798/j.issn.1009-153X.2020.11.011
文献标志码:
A
摘要:
目的 探讨miR-153对胶质母细胞瘤细胞增殖的影响。方法 体外培养胶质母细胞瘤细胞系U87、U251、SHG-44和T98G细胞,分别转染miR-153质粒(miR-153组)、空载质粒(载体组)和miR-153突变质粒(miR-153突变组),另设置空白对照组(不转染任何质粒)。RT-PCR检测miR-153、FOXR2、CDK8和CDK13的表达,MTT法检测细胞增殖能力。结果 miR-153组U87、U251、SHG-44和T98G细胞miR-153表达水平较载体组和空白对照组显著上升(P<0.05),细胞增殖水平较载体组和空白对照组均显著降低(P<0.05)。miR-153组U87、U251、SHG-44和T98G细胞FXOR2、CDK8和CDK13的mRNA水平较miR-153突变组、载体组和空白对照组均显著下降(P<0.05),而后三组之间均无统计学差异(P>0.05)。结论 miR-153抑制胶质母细胞瘤细胞增殖,其机制可能与抑制FXOR2、CDK8和CDK13表达有关。
Abstract:
Objective To study the effect of miR-153 on the cell proliferation of glioblastoma multiforme (GBM) cell lines and its mechanism. Methods GBM cell lines U87, U251, SHG-44 and T98G were cultured in vitro and transfected with miR-153 over-expression plasmid, vector and miR-153 mutant plasmid, respectively. RT-PCR was used to determine the mRNA level of FOXR2, CDK8 and CDK13. MTT assay was used to determine the proliferation of U87, U251, SHG-44 and T98G cells. Results The level of miR-153 and the mRNA level of FOXR2, CDK8 and CDK13 were significantly up-regulated in U87, U251, SHG-44 and T98G cells (P<0.05), and the proliferation of U87, U251, SHG-44 and T98G cells were inhibited after transfected with miR-153 over-expression (P<0.05). Conclusion miR-153 inhibits proliferation of glioblastoma multiforme cell lines, which may be related to the down-regulation of FOXR2, CDK8 and CDK13.

参考文献/References:

[1] Omuro A, DeAngelis LM. Glioblastoma and other malignant gliomas: a clinical review [J]. JAMA, 2013, 310(17): 1842-1850.
[2] Wick W, Osswald M, Wick A, et al. Treatment of glioblasto-ma in adults [J]. Ther Adv Neurol Disord, 2018, 11: 1277012020.
[3] Stupp R, Mason WP, van den Bent MJ, et al. Radiotherapy plus concomitant and adjuvant temozolomide for glioblas-toma [J]. N Engl J Med, 2005, 352(10): 987-996.
[4] Zwirner K, Paulsen F, Schittenhelm J, et al. Prognostic pa-rameters and outcome after re-irradiation for progressive glioblastoma [J]. Acta Neurol Scand, 2017, 136(3): 239-245.
[5] Hammond SM. An overview of microRNAs [J]. Adv Drug Deliv Rev, 2015, 87: 3-14.
[6] Vishnoi A, Rani S. MiRNA biogenesis and regulation of diseases: an overview [J]. Methods Mol Biol, 2017, 1509: 1-10.
[7] Saliminejad K, Khorram KH, Soleymani FS, et al. An over-view of microRNAs: biology, functions, therapeutics, and analysis methods [J]. J Cell Physiol, 2019, 234(5): 5451-5465.
[8] Zhu X, Zhao Y, Hou W, et al. MiR-153 regulates cardio-myocyte apoptosis by targeting Nrf2/HO-1 signaling [J]. Chromosome Res, 2019, 27(3): 167-178.
[9] Huang Q, Xia J, Wang L, et al. miR-153 suppresses IDO1 expression and enhances CAR T cell immunotherapy [J]. J Hematol Oncol, 2018, 11(1): 58.
[10] Bi CW, Zhang GY, Bai Y, et al. Increased expression of miR-153 predicts poor prognosis for patients with prostate cancer [J]. Medicine (Baltimore), 2019, 98(36): e16705.
[11] Olmez I, Love S, Xiao A, et al. Targeting the mesenchymal subtype in glioblastoma and other cancers via inhibition of diacylglycerol kinase alpha [J]. Neuro Oncol, 2018, 20(2): 192-202.
[12] Bhat K, Balasubramaniyan V, Vaillant B, et al. Mesenchy-mal differentiation mediated by NF-kappaB promotes radiation resistance in glioblastoma [J]. Cancer Cell, 2013, 24(3): 331-346.
[13] Chan JA, Krichevsky AM, Kosik KS. MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells [J]. Cancer Res, 2005, 65(14): 6029-6033.
[14] Kefas B, Godlewski J, Comeau L, et al. microRNA-7 inhi-bits the epidermal growth factor receptor and the Akt pathway and is down-regulated in glioblastoma [J]. Cancer Res, 2008, 68(10): 3566-3572.
[15] Lu SQ, Qiu Y, Dai WJ, et al. FOXR2 promotes the prolife-ration, invasion, and epithelial-mesenchymal transition in human colorectal cancer cells [J]. Oncol Res, 2017, 25(5): 681-689.
[16] Liu X, Liu N, Yue C, et al. FoxR2 promotes glioma prolife-ration by suppression of the p27 pathway [J]. Oncotarget, 2017, 8(34): 56255-56266.
[17] Deng X, Hou C, Liang Z, et al. MiR-202 suppresses cell proliferation by targeting foxr2 in endometrial adenocarci-noma [J]. Dis Markers, 2017, 2017: 2827435.
[18] Menzl I, Witalisz-Siepracka A, Sexl V. CDK8-novel thera-peutic opportunities [J]. Pharmaceuticals (Basel), 2019, 12\(2): .
[19] Greifenberg AK, Honig D, Pilarova K, et al. Structural and functional analysis of the Cdk13/Cyclin K complex [J]. Cell Rep, 2016, 14(2): 320-331.
[20] Liang J, Chen M, Hughes D, et al. CDK8 Selectively pro-motes the growth of colon cancer metastases in the liver by regulating gene expression of TIMP3 and matrix metallo-proteinases [J]. Cancer Res, 2018, 78(23): 6594-6606.
[21] Quereda V, Bayle S, Vena F, et al. Therapeutic targeting of CDK12/CDK13 in triple-negative breast cancer [J]. Cancer Cell, 2019, 36(5): 545-558.

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备注/Memo

备注/Memo:
通讯作者:敖祥生,E-mail:aoxiangsheng@sohu.com
更新日期/Last Update: 2020-11-25