[1]陈　波　云亚滨　杜俊峰　王伟志.lncRNA SNHG16对胶质瘤U251细胞增殖、侵袭和迁移的影响[J].中国临床神经外科杂志,2020,(11):771-773.[doi:doi:10.13798/j.issn.1009-153X.2020.11.012]
　CHEN Bo,YUN Ya-bin,DU Jun-feng,et al.Effect of lncRNA SNHG16 on proliferation, invasion and migration of glioma U251 cells[J].,2020,(11):771-773.[doi:doi:10.13798/j.issn.1009-153X.2020.11.012]
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lncRNA SNHG16对胶质瘤U251细胞增殖、侵袭和迁移的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2020年11期
页码:
771-773
栏目:
实验研究
出版日期:
2020-11-25

文章信息/Info

Title:
Effect of lncRNA SNHG16 on proliferation, invasion and migration of glioma U251 cells
文章编号:
1009-153X（2020）11-0771-03
作者:
陈　波　云亚滨　杜俊峰　王伟志
010000 内蒙古，呼和浩特市第一医院神经外科（陈　波、云亚滨、杜俊峰、王伟志）
Author(s):
CHEN Bo;  YUN Ya-bin;  DU Jun-feng;  WANG Wei-zhi.
Department of Neurosurgery, Hohhot First Hospital, Hohhot 010000, China
关键词:
胶质瘤; U251细胞; 长链非编码RNA; 核内小RNA宿主基因16; 细胞增殖; 细胞侵袭; 细胞迁移
Keywords:
Glioma;  U251 cell;  Long chain noncoding RNA;  Small nucleolar RNA host gene 16
分类号:
R 739.41; Q 786
DOI:
doi:10.13798/j.issn.1009-153X.2020.11.012
文献标志码:
A
摘要:
目的 探讨沉默长链非编码RNA核内小RNA宿主基因16（SNHG16）对胶质瘤U251细胞增殖、侵袭和迁移的影响。方法 实时荧光定量PCR检测正常胶质细胞HEB、NHA和胶质瘤细胞A172、U251、U87、SHG-4中SNHG16的表达。用siRNA沉默U251细胞SNHG16的表达，分为NC-siRNA组和SNHG16-siRNA组，CCK-8法检测细胞增殖，Transwell实验检测细胞侵袭，划痕实验检测细胞迁移。结果 与正常胶质细胞HEB和NHA比较，胶质瘤细胞A172、U251、U87和SHG-4的SNHG16表达水平明显升高（P<0.05）。与NC-siRNA组比较，SNHG16-siRNA组细胞增殖能力、细胞侵袭能力和细胞迁移能力均明显降低（P<0.05）。结论 SNHG16在胶质瘤细胞中高表达，特异性沉默SNHG16基因可以抑制胶质瘤细胞的增殖、侵袭和迁移能力。
Abstract:
Objective To explore the effect of long-chain noncoding RNA (lncRNA) small nucleolar RNA host gene 16 (SNHG16) on the proliferation, invasion and migration of glioma U251 cells. Methods Real-time fluorescence quantitative PCR was used to detect the SNHG16 expression in normal gliocytes HEB and NHA and in glioma cell lines A172, U251, U87 and SHG-4. The NC-siRNA (NC-siRNA group) and SNHG16-siRNA (SNHG16-siRNA group) were transfected into U251 cells. CCK-8 assay was used to detect the cell proliferation. Transwell test was used to detect the cell invasion. Cell scratch test was used to detect the cell migration. Results SNHG16 expression levels of glioma cell lines A172, U251, U87 and SHG-4 were significantly increased compared with normal gliocytes HEB and NHA (P<0.05). Compared with NC-siRNA group, the cell abilities of proliferation, invasion and migration were significantly decreased in SNHG16-siRNA group (P<0.05). Conclusion SNHG16 is highly expressed in glioma cells. SNHG16 gene silencing can inhibit the proliferation, invasion and migration of glioma cells.

参考文献/References:

[1] Gusyatiner O, Hegi ME. Glioma epigenetics: from subclassi-fication to novel treatment options [J]. Semin Cancer Biol, 2018, 51: 50-58.
[2] Bhan A, Soleimani M, Mandal SS. Long noncoding RNA and cancer: a new paradigm [J]. Cancer Res, 2017, 77(15): 3965-3981.
[3] Wang X, Kan J, Han J, et al. LncRNA SNHG16 functions as an oncogene by sponging miR-135a and promotes JAK2/STAT3 signal pathway in gastric cancer [J]. J Cancer, 2019, 10(4): 1013-1022.
[4] Xu F, Zha G, Wu Y, et al. Overexpressing lncRNA SNHG16 inhibited HCC proliferation and chemoresistance by func-tionally sponging hsa-miR-93 [J]. Onco Targets Ther, 2018, 11: 8855-8863.
[5] Liu S, Zhang W, Liu K, et al. LncRNA SNHG16 promotes tumor growth of pancreatic cancer by targeting miR-218-5p[J]. Biomed Pharmacother, 2019, 114: 1-8.
[6] 滕　浩，薛一雪，王　萍，等. miR-194对人脑胶质瘤U87细胞恶性生物学行为的影响[J]. 中国医科大学学报，2018，47（8）：673-677.
[7] 付胜楠，张国光，庹　珏，等. 小檗碱及其亲脂性衍生物抑制恶性胶质瘤C6细胞的增殖及迁移与侵袭[J]. 中山大学学报（医学科学版），2013，34（4）：531-536.
[8] 王嘉新，姜兴明. 肿瘤发生发展中SNHG16的调控作用及机制研究[J]. 现代医学，2019，47（3）：350-353.
[9] Gong CY, Tang R, Nan W, et al. Role of SNHG16 in human cancer [J]. Clin Chim Acta, 2020, 503: 175-180.
[10] Zhou XY, Liu H, Ding ZB, et al. lncRNA SNHG16 promotes glioma tumorigenicity through miR-373/EGFR axis by activating PI3K/AKT pathway [J]. Genomics, 2020, 112(1): 1021-1029.
[11] Christensen LL, True K, Hamilton MP, et al. SNHG16 isregulated by the Wnt pathway in colorectal cancer and affects genes involved in lipid metabolism [J]. Mol Oncol, 2016, 10(8): 1266-1282.
[12] Zhou C, Zhao J, Liu J, et al. LncRNA SNHG16 promotesepithelial- mesenchymal transition via down-regulation of DKK3 in gastric cancer [J]. Cancer Biomark, 2019, 26(4):393-401.
[13] 朱晓鸿，赵娟娟，刘娟娟，等. 下调SNHG16对胃癌细胞HGC-27细胞周期的影响[J]. 贵州医药，2017，41（12）：1242-1244.
[14] An JH, Chen ZY, Ma QL, et al. LncRNA SNHG16 promoted proliferation and inflammatory response of macrophages through miR-17-5p/NF-κB signaling pathway in patients with atherosclerosis [J]. Eur Rev Med Pharmacol Sci, 2019, 23(19): 8665-8677.

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备注/Memo

备注/Memo:
通讯作者：王伟志，E-mail：164890480@qq.com
更新日期/Last Update: 2020-11-25